Expression Systems
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Expression Systems
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Author : Michael Dyson
language : en
Publisher: Scion Publishing Ltd
Release Date : 2007-09-15
Expression Systems written by Michael Dyson and has been published by Scion Publishing Ltd this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-15 with Science categories.
1. Expression strategy (Michael Dyson) 2. Protein expression in Escherichia coli (Rosalind Kim) 3. Expression engineering of synthetic antibodies using ribosome display (Matthew DeLisa and Lydia M. Contreras Martinez) 4. Refolding proteins from inclusion bodies (Renaud Vincentelli) 5. Selection of protein variants with improved expression using GFP-derived folding and solubility reporters (Geoffrey Waldo and Stéphanie Cabantous) 6. Protein expression in the wheat germ cell-free system (Yaeta Endo and Tatsuya Sawasaki) 7. Saccharomyces cerevisiae ; A microbial eukaryotic expression system (Christine Lang) 8. Expression of proteins in Pichia pastoris (Geoff and Joan Lin-Cereghino and Wilson Leung) 9. Improved baculovirus expression vectors (Linda King, Richard Hitchman and Robert Possee) 10. Transient transfection of insect cells for rapid expression screening and protein production (Robert Novy et al.) 11. Generation of stable CHO cell lines for protein expression (Zhijian Lu et al.) 12. Transient expression in HEK293-EBNA1 cells (Yves Durocher, Roseanne Tom and Louis Bisson) 13. Nisin- and subtilin-controlled gene expression systems for Gram-positive bacteria (Oscar Kuipers and Jan Kok) 14. Protein expression using lentiviral vectors (Bernard Massie, Renald Gilbert and Sophie Broussau) 15. Expression in mammalian cells using BacMam viruses (Yu-Chen Hu and Hsiao-Ping Lee) List of suppliers;Index
Gene Expression Systems
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Author : Joseph M. Fernandez
language : en
Publisher: Elsevier
Release Date : 1998-12-21
Gene Expression Systems written by Joseph M. Fernandez and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-12-21 with Science categories.
Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Provides detailed information on expression systems Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs Includes timelines and examples Compares pros and cons of each method
Production Of Recombinant Proteins
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Author : Gerd Gellissen
language : en
Publisher: John Wiley & Sons
Release Date : 2006-03-06
Production Of Recombinant Proteins written by Gerd Gellissen and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-06 with Science categories.
While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems -- mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example. With a foreword by Herbert P. Schweizer, Colorado State University, USA: "As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."
Protein Expression Technologies
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Author : François Baneyx
language : en
Publisher: Garland Science
Release Date : 2004
Protein Expression Technologies written by François Baneyx and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Medical categories.
Advances in protein expression technologies have mushroomed in recent years. In this book current and emerging expression technologies are reviewed. Reviews of the molecular genetics of expression systems in various organisms are presented. Topics covered include: Expression of extremophilic proteins; expression in E. coli, Bacillus spp., Saccharomyces cerevisiae, and methylotrophic yeasts; insect cell expression and the baculovirus system; and Chinese Hamster Ovary (CHO) cell lines for large-scale protein production. Also covered are two emerging expression systems, Methylobacterium extorquens AM1 and Caulobacter crescentus.
Baculovirus Expression Systems And Biopesticides
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Author : Michael L. Shuler
language : en
Publisher: Wiley-Liss
Release Date : 1995
Baculovirus Expression Systems And Biopesticides written by Michael L. Shuler and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Science categories.
Developing effective baculovirus-insect culture systems. Insect cell culture methods and their use in virus research. Comparison of mammalian and insect cell cultures. protein production and processing from baculovirus. Development and testing of genetically improved baculovirus insecticides. Fundamentals of baculovirus-insect cell attachment and infection. Development and evaluation of host insect cells. Bioreactor design and scale-up issues. The effect of hydrodynamic forces on insect cells. Commercial application of insect cell culture. Baculovirus-mediated production of proteins in insect cells. Potential application of insect cell-based expression systems in the bio/pharmaceutical industry.
Gene Expression Systems In Fungi Advancements And Applications
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Author : Monika Schmoll
language : en
Publisher: Springer
Release Date : 2016-04-04
Gene Expression Systems In Fungi Advancements And Applications written by Monika Schmoll and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-04 with Science categories.
Biotechnology has emerged as one of the key environmentally safe technologies for the future which enables use of biomass to develop novel smart materials and to replace oil derived products. Fungi are the most efficient producers of the enzymes needed for this purpose and in addition they produce a plethora of secondary metabolites, among which novel antibiotics can be found. Industrial application and exploitation of the metabolic capacities of fungi requires highly productive and robust gene expression systems, which can be achieved by selection of appropriate species and strain improvement. In this book we aim to summarize homologous and heterologous gene expression systems of fungi for production of enzymes and secondary metabolites. A broad overview on requirements, challenges and successful applications shall serve as a basis for further development of fungi as biotechnological workhorses in research and industry.
Cell Free Protein Expression
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Author : W. Antoni Kudlicki
language : en
Publisher: CRC Press
Release Date : 2007-11-27
Cell Free Protein Expression written by W. Antoni Kudlicki and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-27 with Science categories.
Following its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commerica
The Baculovirus Expression System
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Author : Linda A. King
language : en
Publisher: Springer Science & Business Media
Release Date : 1992
The Baculovirus Expression System written by Linda A. King and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Science categories.
A practical guide covering the background, methodological details, applications, advantages and disadvantages of the use of baculovirus expression systems, designed to teach the first-time user the techniques and to highlight more advanced applications for the experienced user.
Membrane Protein Expression Systems
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Author : Gwyn W. Gould
language : en
Publisher: Ashgate Publishing
Release Date : 1994
Membrane Protein Expression Systems written by Gwyn W. Gould and has been published by Ashgate Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Science categories.
The use of heterologous expression as a means to study membrane proteins is becoming widespread. This book is a simple to read laboratory manual describing the principles behind the commonly used expression systems, eg baculovirus, oocytes, cell culture systems and yeast.
Expression Systems And Processes For Rdna Products
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Author : Randolph T. Hatch
language : en
Publisher:
Release Date : 1991
Expression Systems And Processes For Rdna Products written by Randolph T. Hatch and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991 with Reference categories.
Eight papers from a symposium (date and place not cited) explore attempts to expand the range of hosts used for the production of recombinant proteins. They consider bacteria, yeast, and insect cells as hosts; and examine several process techniques. Annotation copyrighted by Book News, Inc., Portland, OR