Gene Targeting In Mammalian Embryonic Stem Cells
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Gene Targeting And Embryonic Stem Cells
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Author : Jim Mcwhir
language : en
Publisher: Taylor & Francis
Release Date : 2004-03-01
Gene Targeting And Embryonic Stem Cells written by Jim Mcwhir and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-03-01 with Science categories.
Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.
Gene Targeting In Mammalian Embryonic Stem Cells
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Author : Kevin J. Spring
language : en
Publisher:
Release Date : 1996
Gene Targeting In Mammalian Embryonic Stem Cells written by Kevin J. Spring and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Embryonic stem cells categories.
Gene Targeting In Mouse Embryonic Stem Cells
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Author : Laura Hink Reid
language : en
Publisher:
Release Date : 1990
Gene Targeting In Mouse Embryonic Stem Cells written by Laura Hink Reid and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with categories.
Gene Targeting
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Author : Alexandra Joyner
language : en
Publisher: OUP Oxford
Release Date : 1999-12-09
Gene Targeting written by Alexandra Joyner and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-12-09 with Science categories.
Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.
Embryonic Stem Cells
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Author : Kursad Turksen
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Embryonic Stem Cells written by Kursad Turksen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
It is fair to say that embryonic stem (ES) cells have taken their place beside the human genome project as one of the most discussed biomedical issues of the day. It also seems certain that as this millennium unfolds we will see an increase in scientific and ethical debate about their potential utility in society. On the scientific front, it is clear that work on ES cells has already generated new possibilities and stimulated development of new strategies for increasing our und- standing of cell lineages and differentiation. It is not naïve to think that, within a decade or so, our overall understanding of stem cell biology will be as revolutionized as it was when the pioneering hemopoietic stem cell studies of Till and McCulloch in Toronto captured our imaginations in 1961. With it will come better methods for ES and lineage-specific stem cell identification, maintenance, and controlled fate selection. Clearly, ES cell models are already providing opportunities for the est- lishment of limitless sources of specific cell populations. In recognition of the gr- ing excitement and potential of ES cells as models for both the advancement of basic science and future clinical applications, I felt it timely to edit this collection of pro- cols (Embryonic Stem Cells) in which forefront investigators would provide detailed methods for use of ES cells to study various lineages and tissue types.
Creating Targeted Mutations In Mouse Embryonic Stem Cells Using Yeast Artificial Chromosomes
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Author : Rebecca Michelle Tucker
language : en
Publisher:
Release Date : 1997
Creating Targeted Mutations In Mouse Embryonic Stem Cells Using Yeast Artificial Chromosomes written by Rebecca Michelle Tucker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
Gene Targeting In Embryonic Stem Cells
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Author : Nicola Zink
language : en
Publisher:
Release Date : 2001
Gene Targeting In Embryonic Stem Cells written by Nicola Zink and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with categories.
Transcriptional And Translational Regulation Of Stem Cells
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Author : Gary Hime
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-05-22
Transcriptional And Translational Regulation Of Stem Cells written by Gary Hime and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-05-22 with Medical categories.
This volume describes the latest findings on transcriptional and translational regulation of stem cells. Both transcriptional activators and repressors have been shown to be crucial for the maintenance of the stem cell state. A key element of stem cell maintenance is repression of differentiation factors or developmental genes – achieved transcriptionally, epigenetically by the Polycomb complex, and post-transcriptionally by RNA-binding proteins and microRNAs. This volume takes two approaches to this topic – (1) illustrating the general principles outlined above through a series of different stem cell examples – embryonic, iPS and adult stem cells, and (2) describing several molecular families that have been shown to have roles in regulation of multiple stem cell populations.
Transgenesis Techniques
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Author : Alan R. Clarke
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Transgenesis Techniques written by Alan R. Clarke and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
The past decade has witnessed a spectacular explosion in both the devel- ment and use of transgenic technologies. Not only have these been used to aid our fundamental understanding of biologic mechanisms, but they have also faci- tated the development of a range of disease models that are now truly beginning to impact upon our approach to human disease. Some of the most exciting model systems relate to neurodegenerative disease and cancer, where the availability of appropriate models is at last allowing radically new therapies to be developed and tested. This latter point is of particular significance given the current concerns of the wider public over both the use of animal models and the merits of using genetically modified organisms. Arguably, advances of the greatest significance have been made using mammalian systems—driven by the advent of embryonic stem-cell–based strategies and, more recently, by cloning through nuclear transfer. For this reason, this new edition of Transgenesis Techniques focuses much more heavily on manipulation of the mammalian genome, both in the general discussions and in the provision of specific protocols.
Gene Targeting In Embryonic Stem Cells And The Manipulation Of Milk Composition
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Author : Satish Kumar
language : en
Publisher:
Release Date : 1994
Gene Targeting In Embryonic Stem Cells And The Manipulation Of Milk Composition written by Satish Kumar and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Genetic engineering categories.