Handbook Of Proteomic Methods
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Handbook Of Proteomic Methods
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Author : P. Michael Conn
language : en
Publisher: Springer Science & Business Media
Release Date : 2003-07-10
Handbook Of Proteomic Methods written by P. Michael Conn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-07-10 with Science categories.
A compendium of thirty-four powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. Thee readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, posttranslational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology.
The Proteomics Protocols Handbook
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Author : John M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-09
The Proteomics Protocols Handbook written by John M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-09 with Science categories.
Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Proteomics
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Author : C. David O'Connor
language : en
Publisher: Scion Publishing Ltd
Release Date : 2007-11-01
Proteomics written by C. David O'Connor and has been published by Scion Publishing Ltd this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-01 with Science categories.
Proteomics: Methods Express identifies the most powerful new technologies and presents them in a way that allows their robust implementation. The focus is on proteomic methods and strategies that are reliable and of general applicability. Each chapter presents descriptions of what can, and cannot, be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house. Every chapter discusses the merits and limitations of various approaches then provides tried-and-tested protocols with hints and tips for success and troubleshooting for when things go wrong.
Proteins And Proteomics
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Author : Richard J. Simpson
language : en
Publisher: CSHL Press
Release Date : 2003
Proteins And Proteomics written by Richard J. Simpson and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Proteins categories.
Introduction to proteomics; one-dimensional polyacrylamide gel electrophoresis; preparing cellular and subcellular extracts; preparative two-dimensional gel electrophoresis with immobilized pH gradients; reversed-phase high -performance liquid chromatography; amino- and carboxy-terminal sequence analysis; peptide mapping and sequence analysis of gel-resolved proteins; the use of mass spectrometry in proteomics; proteomic methods for phosphorylation site mapping; characterization of protein complexes; making sense of proteomics - using bioinformatics to discover a protein's structure, functions, and interactions.
Purifying Proteins For Proteomics
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Author : Richard J. Simpson
language : en
Publisher: CSHL Press
Release Date : 2004
Purifying Proteins For Proteomics written by Richard J. Simpson and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Biochemistry categories.
This manual complements Simpson's Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The chapters include detailed protocols, methods for optimizing the performance of experiments, discussion of potential pitfalls, and troubleshooting advice.
Proteomics In Practice
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Author : Reiner Westermeier
language : en
Publisher: John Wiley & Sons
Release Date : 2008-09-08
Proteomics In Practice written by Reiner Westermeier and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-09-08 with Science categories.
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
Handbook Of Molecular And Cellular Methods In Biology And Medicine
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Author : Leland J. Cseke
language : en
Publisher: CRC Press
Release Date : 2003-11-24
Handbook Of Molecular And Cellular Methods In Biology And Medicine written by Leland J. Cseke and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-11-24 with Medical categories.
Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sa
Proteomics In Practice
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Author : Reiner Westermeier
language : en
Publisher: Wiley-Blackwell
Release Date : 2008-05-05
Proteomics In Practice written by Reiner Westermeier and has been published by Wiley-Blackwell this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-05-05 with Science categories.
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR. Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
Serum Plasma Proteomics
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Author : David W. Greening
language : en
Publisher: Springer Nature
Release Date : 2023-02-13
Serum Plasma Proteomics written by David W. Greening and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-13 with Science categories.
This third volume provides comprehensive protocols on pre-analytical, analytical, plasma, and serum proteomics. New and updated chapters are divided into nine sections, detailing blood processing and handling strategies, discovery- and targeted-based mass spectrometry, including workflows to aid in discovery and targeted data analysis, in addition to software and bioinformatics for the plasma proteome. This edition further integrates emerging areas in the development of technologies for plasma proteomics and assay platforms in biomarker discovery and translational proteomics, enrichment and detection strategies to understand the plasma proteome, and peptide, lipid and metabolite targeted assays. We also detail the emerging analysis of extracellular vesicles isolated from plasma. Written in the format of the highly successful Methods in Molecular Biology series, each of the 33 chapters includes an introduction to the topic, lists necessary materials and methods, includes hints and tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Serum/Plasma Proteomics: Methods and Protocols, Third Edition aims to be comprehensive guide for researchers.
Quantitative Proteome Analysis
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Author : Kazuhiro Imai
language : en
Publisher: CRC Press
Release Date : 2013-08-05
Quantitative Proteome Analysis written by Kazuhiro Imai and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-08-05 with Science categories.
This book focuses on the advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and reproducibility. It also concentrates on the effective applications of these methods that resulted in many discoveries of the role of the proteins expressed in living cells and biological fluids. The first part of the book focuses on the description of advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and, especially, reproducibility. The second part of the book focuses on providing concise descriptions of the effective applications of these methods to demonstrate how they have resulted in many important discoveries of the roles of the proteins expressed in living cells.