High Performance Gradient Elution

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High Performance Gradient Elution

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Author : Lloyd R. Snyder
language : en
Publisher: John Wiley & Sons
Release Date : 2007-01-09

High Performance Gradient Elution written by Lloyd R. Snyder and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-01-09 with Science categories.

Gradient elution demystified Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed. Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. The book presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool. This up-to-date, practical, and comprehensive treatment of gradient elution: * Provides specific, step-by-step recommendations for developing a gradient separation for any sample * Describes the best approach for troubleshooting problems with gradient methods * Guides the reader on the equipment used for gradient elution * Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients * Explains how to avoid problems in transferring gradient methods With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool.

High Performance Liquid Chromatography Capillary Electrophoresis

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Author : Andrea Weston
language : en
Publisher: Elsevier
Release Date : 1997-07-21

High Performance Liquid Chromatography Capillary Electrophoresis written by Andrea Weston and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-07-21 with Science categories.

HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity.Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. - Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques - Applicable to a wide variety of separation problems - Covers basic concepts governing any separation as well as instrumentation and how to use it - Helps the user to obtain optimal resolution in minimal time - Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation - Includes information on upcoming trends such as miniaturization - Major concepts in each chapter are organized to allow access to information easily and quickly - Contains practical bibliography for accessing the literature

Separation Methods In Drug Synthesis And Purification

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Author : Klara Valko
language : en
Publisher: Elsevier
Release Date : 2020-06-19

Separation Methods In Drug Synthesis And Purification written by Klara Valko and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-19 with Science categories.

Separation Methods in Drug Synthesis and Purification, Second Edition, Volume Eight, provides an updated on the analytical techniques used in drug synthesis and purification. Unlike other books on either separation science or drug synthesis, this volume combines the two to explain the basic principles and comparisons of each separation technique. New sections to this volume include enantiomer separation using capillary electrophoresis (CE) and capillary electro- chromatography, the computer simulation of chromatographic separation for accelerating method development, the application of chromatography and capillary electrophoresis used as surrogates for biological processes, and new developments in the established techniques of chromatography and preparative methods. - Features descriptions and applications of all separation methods used in the pharmaceutical industry - Written by the leading scientists in their respective fields, providing solutions for a wide range of industrial separation problems encountered within the pharmaceutical industry - Thoroughly updated with brand new separation science techniques and the latest developments in the established techniques of chromatography

High Performance Liquid Chromatography In Pesticide Residue Analysis

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Author : Tomasz Tuzimski
language : en
Publisher: CRC Press
Release Date : 2015-05-20

High Performance Liquid Chromatography In Pesticide Residue Analysis written by Tomasz Tuzimski and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-05-20 with Science categories.

HPLC is the principal separation technique for identification of the pesticides in environmental samples and for quantitative analysis of analytes. At each stage of the HPLC procedure, the chromatographer should possess both the practical and theoretical skills required to perform HPLC experiments correctly and to obtain reliable, repeatable, and r

Gradient Hplc For Practitioners

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Author : Stavros Kromidas
language : en
Publisher: John Wiley & Sons
Release Date : 2019-04-04

Gradient Hplc For Practitioners written by Stavros Kromidas and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-04 with Science categories.

This practical guide for analytical scientists explains the use of gradients in liquid chromatography. The fundamentals of gradient separations, as well as the most common application scenarios are addressed, from LC-MS coupling to biochromatography to the separation of ionic substances. Throughout, this handy volume provides detailed hands-on information for practitioners, enabling them to use gradient separation methods reliably and efficiently.

Advances In Chromatography

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Author : Nelu Grinberg
language : en
Publisher: CRC Press
Release Date : 2021-12-19

Advances In Chromatography written by Nelu Grinberg and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-12-19 with Science categories.

For six decades, scientists and researchers have relied on the Advances in Chromatography series for the most up-to date information on a wide range of developments in chromatographic methods and applications. The clear presentation of topics and vivid illustrations for which this series has become known makes the material accessible and engaging to analytical, biochemical, organic, polymer, and pharmaceutical chemists at all levels of technical skill. Key Features: Discusses the basic concepts of affinity chromatography and examines recent developments in this method and related supramolecular separation methods. Outlines the different types of gradient stationary phases and how they have been used in and benefited the field of separation science. Reviews recent trends in detectors for GC, focusing on those that are readily available and seeing wide usage. Addresses peak compression in GELC and offers the reader a plate height equation to work with that incorporates its effects.

Protein Liquid Chromatography

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Author : M. Kastner
language : en
Publisher: Elsevier
Release Date : 2000

Protein Liquid Chromatography written by M. Kastner and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Science categories.

Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Handbook Of Ion Chromatography 3 Volume Set

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Author : Joachim Weiss
language : en
Publisher: John Wiley & Sons
Release Date : 2016-09-26

Handbook Of Ion Chromatography 3 Volume Set written by Joachim Weiss and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-26 with Science categories.

This three-volume handbook is the standard reference in the field, unparalleled in its comprehensiveness. It covers every conceivable topic related to the expanding and increasingly important field of ion chromatography. The fourth edition is completely updated and revised to include the latest developments in the instrumentation, now stretching to three volumes to reflect the current state of applications. Ion chromatography is one of the most widely used separation techniques of analytical chemistry with applications in fields such as medicinal chemistry, water chemistry and materials science. Consequently, the number of users of this method is continuously growing, underlining the need for an up-to-date reference. A true pioneer of this method, Joachim Weiss studied chemistry at the Technical University of Berlin (Germany), where he also received his PhD degree in Analytical Chemistry. In 2002, he did his habilitation in Analytical Chemistry at the Leopold-Franzens University in Innsbruck (Austria), where he is also teaching liquid chromatography. Since 1982, Dr. Weiss has worked at Dionex (now being part of Thermo Fisher Scientific), where he currently holds the position of Technical Director for Dionex Products within the Chromatography and Mass Spectrometry Division (CMD) of Thermo Fisher Scientific, located in Dreieich (Germany).

Multi Dimensional Liquid Chromatography

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Author : Dwight R. Stoll
language : en
Publisher: CRC Press
Release Date : 2022-12-30

Multi Dimensional Liquid Chromatography written by Dwight R. Stoll and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-12-30 with Science categories.

Two-dimensional liquid chromatography (2D-LC) is finding increasingly wide application principally due to the analysis of mixtures of moderate to high complexity. Many industries are developing increasingly complex products that are challenging the separation capabilities of state-of-the-art 1D-LC and need new analytical methodologies with substantially more resolving power, and 2D-LC meets that need. This text, organized by two leaders in the field, establishes a sound fundamental basis for the principles of the technique, followed by a discussion of important practical considerations. The book begins with an introduction to multi-dimensional separations and a discussion of the history and development of the technique over the past 40 years, followed by several chapters that provide a theoretical basis for development of 2D-LC methods, including foundational concepts regarding separation complementarity, under-sampling, and dynamics of liquid chromatography separations. Instrumentation for 2D-LC is discussed extensively, including practical aspects such as interface selection and setup. Building on this foundation, two separate chapters are focused on method development for non-comprehensive and comprehensive separations, followed by a chapter dedicated to data analysis. Finally, applications of 2D-LC in several fields ranging from pharmaceutical analysis to polymer science are summarized. The book is an important resource for both students and practitioners who are already using 2D-LC or are interested in getting started in the field. Key Features: Demonstrates the conditions under which a 2D-LC method should be considered as an alternative to a 1D-LC method Establishes a sound fundamental basis of the principles of the technique, followed by guidelines for method optimization Provides a single source for technical knowledge advances and practical guidance described in recent literature Assists with the initial decision to develop a 2D-LC method Guides the reader in developing a high-quality method that meets the needs of their application

High Performance Liquid Chromatography In Phytochemical Analysis

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Author : Monika Waksmundzka-Hajnos
language : en
Publisher: CRC Press
Release Date : 2010-11-08

High Performance Liquid Chromatography In Phytochemical Analysis written by Monika Waksmundzka-Hajnos and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-08 with Health & Fitness categories.

The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp