High Performance Liquid Chromatography In Enzymatic Analysis
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Hplc In Enzymatic Analysis
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Author : Edward F. Rossomando
language : en
Publisher: John Wiley & Sons
Release Date : 2009-09-25
Hplc In Enzymatic Analysis written by Edward F. Rossomando and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-09-25 with Science categories.
The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.
High Performance Liquid Chromatography In Enzymatic Analysis
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Author : Edward F. Rossomando
language : en
Publisher: Wiley-Interscience
Release Date : 1987-05-04
High Performance Liquid Chromatography In Enzymatic Analysis written by Edward F. Rossomando and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987-05-04 with Science categories.
Describes the high performance liquid chromatography (hplc) method and thoroughly explains and illustrates its use in the analysis of enzymatic activities, rather than its traditional use as a tool for separation. This change in emphasis is particularly attractive to those who wish to make use of the activity of an enzyme as an indicator of cellular function, a determinant of a given stage of differentiation (or dedifferentiation), or even as a measure of gene function. Presentation focuses on the assay of the activity of an enzyme rather than its purification. Each chapter deals with a different aspect of the method, beginning with an overview and ending with a detailed summary. The final chapter treats the growing field of assay of multienzyme systems. Includes more than 170 illustrations reproduced from the original research literature.
Hplc Of Peptides And Proteins
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Author : Marie-Isabel Aguilar
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Hplc Of Peptides And Proteins written by Marie-Isabel Aguilar and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
High Performance Liquid Chromatography In Phytochemical Analysis
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Author : Monika Waksmundzka-Hajnos
language : en
Publisher: CRC Press
Release Date : 2010-11-08
High Performance Liquid Chromatography In Phytochemical Analysis written by Monika Waksmundzka-Hajnos and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-08 with Health & Fitness categories.
The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp
Steroid Analysis
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Author : Hugh L. J. Makin
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Steroid Analysis written by Hugh L. J. Makin and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
reviews of the first edition -- `...this is an excellent, comprehensive book and can be highly recommended to those who want an up-to-date reference on steroid analysis.' Analyst
Chromatography Of Lipids In Biomedical Research And Clinical Diagnosis
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Author : A. Kuksis
language : en
Publisher: Elsevier
Release Date : 1987-01-01
Chromatography Of Lipids In Biomedical Research And Clinical Diagnosis written by A. Kuksis and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987-01-01 with Science categories.
This multi-author volume provides an unprecedented in-depth coverage of the separation, identification and quantitation of simple and complex food and tissue fats and other lipids by chromatographic and mass spectrometric methods with emphasis on applications to biomedical research and clinical diagnosis. It contains a total of 13 chapters written by scientists who are internationally recognized in their specific fields. The volume covers analyses of molecular species of eicosanoids, sterols, and glycero and glycolipids in healthy and diseased human tissues and in appropriate animal models. The text complements the simpler group determinations of lipid classes commonly employed in clinical laboratories. The volume anticipates the discovery of the specific metabolism of molecular species of complex glycerolipids and provides the medical researcher and clinical investigator with the means for dealing with it.
Ultra Performance Liquid Chromatography Mass Spectrometry
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Author : Mu Naushad
language : en
Publisher: CRC Press
Release Date : 2014-03-18
Ultra Performance Liquid Chromatography Mass Spectrometry written by Mu Naushad and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-03-18 with Science categories.
Due to its high sensitivity and selectivity, liquid chromatography–mass spectrometry (LC–MS) is a powerful technique. It is used for various applications, often involving the detection and identification of chemicals in a complex mixture. Ultra Performance Liquid Chromatography Mass Spectrometry: Evaluation and Applications in Food Analysis presents a unique collection of up-to-date UPLC-MS/MS methods for the separation and quantitative determination of components, contaminants, vitamins, and aroma and flavor compounds in a wide variety of foods and food products. The book begins with an overview of the history, principles, and advancement of chromatography. It discusses the use of UHPLC techniques in food metablomics, approaches for analysis of foodborne carcinogens, and details of UPLC-MS techniques used for the separation and determination of capsaicinoids. Chapters describe the analysis of contaminants in food, including pesticides, aflatoxin, perfluorochemicals, and acrylamide, as well as potentially carcinogenic heterocyclic amines in cooked foods. The book covers food analysis for beneficial compounds, such as the determination of folate, vitamin content analysis, applications for avocado metabolite studies, virgin olive oil component analysis, lactose determination in milk, and analysis of minor components of cocoa and phenolic compounds in fruits and vegetables. With contributions by experts in interdisciplinary fields, this reference offers practical information for readers in research and development, production, and routing analysis of foods and food products.
Modern Chromatographic Analysis Of Vitamins
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Author : Andre P. De Leenheer
language : en
Publisher: CRC Press
Release Date : 2000-04-18
Modern Chromatographic Analysis Of Vitamins written by Andre P. De Leenheer and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-04-18 with Science categories.
Third Edition collects and examines the tremendous proliferation of information on chromatographic analysis of fat and water soluble vitamins over the last decade. Extensively describes sample preparation and final measurement.
Liquid Chromatography In Clinical Analysis
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Author : Pokar M. Kabra
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-07
Liquid Chromatography In Clinical Analysis written by Pokar M. Kabra and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-07 with Science categories.
Liquid Chromatography in Clinical Analysis
Protein And Peptide Analysis By Lc Ms
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Author : Thomas Letzel
language : en
Publisher: Royal Society of Chemistry
Release Date : 2011
Protein And Peptide Analysis By Lc Ms written by Thomas Letzel and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Science categories.
This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.