Hplc In Enzymatic Analysis
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Hplc In Enzymatic Analysis
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Author : Edward F. Rossomando
language : en
Publisher: John Wiley & Sons
Release Date : 2009-09-25
Hplc In Enzymatic Analysis written by Edward F. Rossomando and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-09-25 with Science categories.
The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.
High Performance Liquid Chromatography In Enzymatic Analysis
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Author : Edward F. Rossomando
language : en
Publisher: Wiley-Interscience
Release Date : 1987-05-04
High Performance Liquid Chromatography In Enzymatic Analysis written by Edward F. Rossomando and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987-05-04 with Science categories.
Describes the high performance liquid chromatography (hplc) method and thoroughly explains and illustrates its use in the analysis of enzymatic activities, rather than its traditional use as a tool for separation. This change in emphasis is particularly attractive to those who wish to make use of the activity of an enzyme as an indicator of cellular function, a determinant of a given stage of differentiation (or dedifferentiation), or even as a measure of gene function. Presentation focuses on the assay of the activity of an enzyme rather than its purification. Each chapter deals with a different aspect of the method, beginning with an overview and ending with a detailed summary. The final chapter treats the growing field of assay of multienzyme systems. Includes more than 170 illustrations reproduced from the original research literature.
Enzyme Assays
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Author : Robert Eisenthal
language : en
Publisher: Practical Approach
Release Date : 2002
Enzyme Assays written by Robert Eisenthal and has been published by Practical Approach this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Medical categories.
Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used to measure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters. Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gel electrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening; statistical analysis of enzyme kinetic data; and the determination of active site concentration. This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.
Handbook Of Dairy Foods Analysis
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Author : Leo M.L. Nollet
language : en
Publisher: CRC Press
Release Date : 2009-11-04
Handbook Of Dairy Foods Analysis written by Leo M.L. Nollet and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-11-04 with Technology & Engineering categories.
Dairy foods account for a large portion of the Western diet, but due to the potential diversity of their sources, this food group often poses a challenge for food scientists and their research efforts. Bringing together the foremost minds in dairy research, Handbook of Dairy Foods Analysis compiles the top dairy analysis techniques and methodologies from around the world into one, well-organized volume. Co-Edited by Fidel Toldra - Recipient of the 2010 Distinguished Research Award from the American Meat Science Association Exceptionally comprehensive both in its detailing of methods and the range of products covered, this handbook includes tools for analyzing chemical and biochemical compounds and also bioactive peptides, prebiotics, and probiotics. It describes noninvasive chemical and physical sensors and starter cultures used in quality control. Covers the Gamut of Dairy Analysis Techniques The book discusses current methods for the detection of microorganisms, allergens, and other adulterations, including those of environmental origin or introduced during processing. Other methodologies used to evaluate color, texture, and flavor are also discussed. Written by an International Panel of Distinguished Contributors Under the editorial guidance of renowned authorities, Leo M.L. Nollet and Fidel Toldrá, this handbook is one of the few references that is completely devoted to dairy food analysis – a extremely valuable reference for those in the dairy research, processing, and manufacturing industries.
Hplc In Enzymatic Analysis
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Author : Edward F. Rossomando
language : en
Publisher: Wiley-Liss
Release Date : 1998-04-21
Hplc In Enzymatic Analysis written by Edward F. Rossomando and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-04-21 with Science categories.
The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.
Advances In Chromatography
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Author : J. Calvin Giddings
language : en
Publisher: CRC Press
Release Date : 2021-07-29
Advances In Chromatography written by J. Calvin Giddings and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-07-29 with Science categories.
This book discusses the principles behind the measurements, the state of the art in technology and possible future developments in liquid chromatography (LC). It explains the limit of detection in LC for considering separately the minimum total quantity and the minimum concentration at the detector.
Handbook Of Oxidants And Antioxidants In Exercise
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Author : C. Sen
language : en
Publisher: Elsevier
Release Date : 2000-02-16
Handbook Of Oxidants And Antioxidants In Exercise written by C. Sen and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-02-16 with Science categories.
Interest in the science of exercise dates back to the time of ancient Greece. Today exercise is viewed not only as a leisurely activity but also as an effective preventive and therapeutic tool in medicine. Further biomedical studies in exercise physiology and biochemistry reports that strenuous physical exercise might cause oxidative lipid damage in various tissues. The generation of reactive oxygen species is elevated to a level that overwhelms the tissue antioxidant defense systems resulting in oxidative stress.The Handbook of Oxidants and Antioxidants in Exercise examines the different aspects of exercise-induced oxidative stress, its management, and how reactive oxygen may affect the functional capacity of various vital organs and tissues. It includes key related issues such as analytical methods, environmental factors, nutrition, aging, organ function and several pathophysiological processes.This timely publication will be of relevance to those in biomedical science and was designed to be readily understood by the general scientific audience.
Handbook Of Analysis Of Active Compounds In Functional Foods
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Author : Leo M.L. Nollet
language : en
Publisher: CRC Press
Release Date : 2012-01-18
Handbook Of Analysis Of Active Compounds In Functional Foods written by Leo M.L. Nollet and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-01-18 with Science categories.
Functional foods offer specific benefits that enhance life and promote longevity, and the active compounds responsible for these favorable effects can be analyzed through a range of techniques. Handbook of Analysis of Active Compounds in Functional Foods presents a full overview of the analytical tools available for the analysis of active ingredients in these products. Nearly 100 experts from all over the world explore an array of methodologies for investigating and evaluating various substances, including: Amino acids, peptides, and proteins, along with glutamine, taurine, glutathione, carnitine, and creatine Water- and fat-soluble vitamins and probiotics Terpenes, including hydrocarbon carotenoids and oxycarotenoids (xanthophylls) Phenolic compounds such as flavonoids, flavan-3-ols, proanthocyanidins, stilbenes, resveratrol, anthocynanins, isoflavones, tannins, ellagic acid, and chlorogenic acids Fibers and polysaccharides, including chitosan, insoluble dietary fiber, fructans, inulin, pectin, and cyclodextrins Phytoestrogens and hormones, with chapters on anise oil and melatonin Tetrapyrroles, minerals, and trace elements Lipid compounds, with discussions of omega 3 and 6 fatty acids, conjugated linoleic acids, lecithin, sterols, stanols, lipoic acid, and alliin Sweeteners, salt replacers, and taste-modifying compounds Each chapter describes the specific compound and its benefits, surveys the range of analytic techniques available, and provides ample references to facilitate further study. The book follows a convenient format with well-organized chapters, allowing readers to quickly hone in on specific topics of interest. This comprehensive reference provides a complete survey of the most cutting-edge analytical techniques available for researchers, industry professionals, and regulators.
Advances In Food And Nutrition Research
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Author : Steve Taylor
language : en
Publisher: Academic Press
Release Date : 2011-05-04
Advances In Food And Nutrition Research written by Steve Taylor and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-05-04 with Cooking categories.
Advances in Food and Nutrition Research recognizes the integral relationship between the food and nutritional sciences and brings together outstanding and comprehensive reviews that highlight this relationship. Contributions detail scientific developments in the broad areas of food science and nutrition and are intended to provide those in academia and industry with the latest information on emerging research in these constantly evolving sciences. The latest important information for food scientists and nutritionists Peer-reviewed articles by a panel of respected scientists The go-to series since 1948
The Greening Of Pharmaceutical Engineering Applications For Physical Disorder Treatments
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Author : M. R. Islam
language : en
Publisher: John Wiley & Sons
Release Date : 2023-03-14
The Greening Of Pharmaceutical Engineering Applications For Physical Disorder Treatments written by M. R. Islam and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-03-14 with Science categories.
The Greening of Pharmaceutical Engineering This fourth volume in a groundbreaking new four-volume set offers new philosophies and ideas for diagnosing and treating physical disorders, with a view toward bringing the pharmaceutical industry closer to chemical, environmental, and economic sustainability. This fourth and final volume in a four-volume series on the greening of pharmaceutical engineering presents evidence for the applications of new theories advanced in the first two volumes. Similar to the Volume three, which was for mental health, this volume presents applications for the diagnosis and treatment of physical disorders. Based on the groundwork laid in the first two volumes, the authors now embark on significant, real-life scenarios that apply their theory to physical disorder treatments. In keeping with the tradition of engineering solutions, practical yet sustainable solutions are presented. The fundamental flaws of the conventional approach to medicine is well known. However, this book does not stop at unearthing criticism of western medicine. It offers hope and recommends a series of solutions for both prevention and treatment of physical ailments. Both evidence in studies and the theory behind various treatments are analyzed. Furthermore, food and energy sources are discussed in general as they both directly impact human health. Physical and emotional activities are the other tangible and intangible factors that play a role in human health are discussed. All major diseases, scientific diagnoses, and their common treatments are presented and analyzed. Recommendations for lifestyle changes that could prevent and reverse syndromes are discussed with comprehensive analysis and evidence in medicinal studies. Seasonal flus as well as Covid-19 and other recent viruses with deadly consequences are discussed and ways to manage them through diet, exercises, and meditation are presented. Treatment plans, along with food recipes for specific ailments are presented with discussion of the logic behind these remedies. Valuable as a learning tool for beginners in this area as well as a daily reference for engineers and scientists working in these areas, this is a must-have for any library.