High Speed Atomic Force Microscopy In Biology
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High Speed Atomic Force Microscopy In Biology
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Author : Toshio Ando
language : en
Publisher: Springer Nature
Release Date : 2022-03-23
High Speed Atomic Force Microscopy In Biology written by Toshio Ando and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-03-23 with Science categories.
This first book on high-speed atomic force microscopy (HS-AFM) is intended for students and biologists who want to use HS-AFM in their research. It provides straightforward explanations of the principle and techniques of AFM and HS-AFM. Numerous examples of HS-AFM studies on proteins demonstrate how to apply this new form of microscopy to specific biological problems. Several precautions for successful imaging and the preparation of cantilever tips and substrate surfaces will greatly benefit first-time users of HS-AFM. In turn, the instrumentation techniques detailed in Chapter 4 can be skipped, but will be useful for engineers and scientists who want to develop the next generation of high-speed scanning probe microscopes for biology. The book is intended to facilitate the first-time use of this new technique, and to inspire students and researchers to tackle their own specific biological problems by directly observing dynamic events occurring in the nanoscopic world. Microscopy in biology has recently entered a new era with the advent of high-speed atomic force microscopy (HS-AFM). Unlike optical microscopy, electron microscopy, and conventional slow AFM, it allows us to directly observe biological molecules in physiological environments. Molecular “movies” created using HS-AFM can directly reveal how molecules behave and operate, without the need for subsequent complex analyses and roundabout interpretations. It also allows us to directly monitor morphological change in live cells, and dynamic molecular events occurring on the surfaces of living bacteria and intracellular organelles. As HS-AFM instruments were recently commercialized, in the near future HS-AFM is expected to become a common tool in biology, and will enhance and accelerate our understanding of biological phenomena.
Atomic Force Microscopy
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Author : Nuno C. Santos
language : en
Publisher: Humana Press
Release Date : 2018-10-30
Atomic Force Microscopy written by Nuno C. Santos and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-30 with Science categories.
This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.
Atomic Force Microscopy In Molecular And Cell Biology
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Author : Jiye Cai
language : en
Publisher: Springer
Release Date : 2018-11-03
Atomic Force Microscopy In Molecular And Cell Biology written by Jiye Cai and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-11-03 with Science categories.
The book addresses new achievements in AFM instruments – e.g. higher speed and higher resolution – and how AFM is being combined with other new methods like NSOM, STED, STORM, PALM, and Raman. This book explores the latest advances in atomic force microscopy and related techniques in molecular and cell biology. Atomic force microscopy (AFM) can be used to detect the superstructures of the cell membrane, cell morphology, cell skeletons and their mechanical properties. Opening up new fields of in-situ dynamic study for living cells, enzymatic reactions, fibril growth and biomedical research, these combined techniques will yield valuable new insights into molecule and cell biology. This book offers a valuable resource for students and researchers in the fields of biochemistry, cell research and chemistry etc.
Atomic Force Microscopy In Liquid
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Author : Arturo M. Baró
language : en
Publisher: John Wiley & Sons
Release Date : 2012-05-14
Atomic Force Microscopy In Liquid written by Arturo M. Baró and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-05-14 with Science categories.
About 40 % of current atomic force microscopy (AFM) research is performed in liquids, making liquid-based AFM a rapidly growing and important tool for the study of biological materials. This book focuses on the underlying principles and experimental aspects of AFM under liquid, with an easy-to-follow organization intended for new AFM scientists. The book also serves as an up-to-date review of new AFM techniques developed especially for biological samples. Aimed at physicists, materials scientists, biologists, analytical chemists, and medicinal chemists. An ideal reference book for libraries. From the contents: Part I: General Atomic Force Microscopy * AFM: Basic Concepts * Carbon Nanotube Tips in Atomic Force Microscopy with * Applications to Imaging in Liquid * Force Spectroscopy * Atomic Force Microscopy in Liquid * Fundamentals of AFM Cantilever Dynamics in Liquid * Environments * Single-Molecule Force Spectroscopy * High-Speed AFM for Observing Dynamic Processes in Liquid * Integration of AFM with Optical Microscopy Techniques Part II: Biological Applications * DNA and Protein-DNA Complexes * Single-Molecule Force Microscopy of Cellular Sensors * AFM-Based Single-Cell Force Spectroscopy * Nano-Surgical Manipulation of Living Cells with the AFM
Atomic Force Microscopy
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Author : Ahmed Touhami
language : en
Publisher: Springer Nature
Release Date : 2022-06-01
Atomic Force Microscopy written by Ahmed Touhami and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-06-01 with Science categories.
Over the last two decades, Atomic Force Microscopy (AFM) has undoubtedly had a considerable impact in unraveling the structures and dynamics of microbial surfaces with nanometer resolution, and under physiological conditions. Moreover, the continuous innovations in AFM-based modalities as well as the combination of AFM with modern optical techniques, such as confocal fluorescence microscopy or Raman spectroscopy, increased the diversity and volume of data that can be acquired in an experiment. It is evident that these combinations provide new ways to investigate a broad spectrum of microbiological processes at the level of single cells. In this book, I have endeavored to highlight the wealth of AFM-based modalities that have been implemented over the recent years leading to the multiparametric and multifunctional characterization of, specifically, bacterial surfaces. Examples include the real-time imaging of the nanoscale organization of cell walls, the quantification of subcellular chemical heterogeneities, the mapping and functional analysis of individual cell wall constituents, and the probing of the nanomechanical properties of living bacteria. It is expected that in the near future more AFM-based modalities and complementary techniques will be combined into single experiments to address pertinent problems and challenges in microbial research. Such improvements may make it possible to address the dynamic nature of many more microbial cell surfaces and their constituents, including the restructuring of cellular membranes, pores and transporters, signaling of cell membrane receptors, and formation of cell-adhesion complexes. Ultimately, manifold discoveries and engineering possibilities will materialize as multiparametric tools allow systems of increasing complexity to be probed and manipulated.
Scanning Probe Microscopy In Nanoscience And Nanotechnology 2
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Author : Bharat Bhushan
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-12-17
Scanning Probe Microscopy In Nanoscience And Nanotechnology 2 written by Bharat Bhushan and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-12-17 with Technology & Engineering categories.
This book presents the physical and technical foundation of the state of the art in applied scanning probe techniques. It constitutes a timely and comprehensive overview of SPM applications. The chapters in this volume relate to scanning probe microscopy techniques, characterization of various materials and structures and typical industrial applications, including topographic and dynamical surface studies of thin-film semiconductors, polymers, paper, ceramics, and magnetic and biological materials. The chapters are written by leading researchers and application scientists from all over the world and from various industries to provide a broader perspective.
Nanocellbiology
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Author : Bhanu P. Jena
language : en
Publisher: CRC Press
Release Date : 2014-04-23
Nanocellbiology written by Bhanu P. Jena and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-04-23 with Medical categories.
This book provides a comprehensive understanding of the discovery of a new cellular structure the "porosome," which is the universal secretory machinery in cells; the protein assembly, biomineralization, and biomolecular interactions; the molecular evolution of protein structure; the use of magnetic nanoparticles for transformative application in medicine and therapy, and the new and novel imaging approach of electrical impedance spectroscopy in biology. It be used for college courses in nanomedicine, nano cell biology, advanced nanotechnology, and biotechnology at the undergraduate and graduate level.
Ion Channels Channel Biochemistry Reconstitution And Function
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Author :
language : en
Publisher: Academic Press
Release Date : 2021-05-29
Ion Channels Channel Biochemistry Reconstitution And Function written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-05-29 with Science categories.
Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series
Atomic Force Microscopy Investigations Into Biology
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Author : Christopher Frewin
language : en
Publisher: BoD – Books on Demand
Release Date : 2012-03-07
Atomic Force Microscopy Investigations Into Biology written by Christopher Frewin and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-03-07 with Medical categories.
The atomic force microscope (AFM) has become one of the leading nanoscale measurement techniques for materials science since its creation in the 1980's, but has been gaining popularity in a seemingly unrelated field of science: biology. The AFM naturally lends itself to investigating the topological surfaces of biological objects, from whole cells to protein particulates, and can also be used to determine physical properties such as Young's modulus, stiffness, molecular bond strength, surface friction, and many more. One of the most important reasons for the rise of biological AFM is that you can measure materials within a physiologically relevant environment (i.e. liquids). This book is a collection of works beginning with an introduction to the AFM along with techniques and methods of sample preparation. Then the book displays current research covering subjects ranging from nano-particulates, proteins, DNA, viruses, cellular structures, and the characterization of living cells.
The Nanobiotechnology Handbook
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Author : Yubing Xie
language : en
Publisher: CRC Press
Release Date : 2012-11-16
The Nanobiotechnology Handbook written by Yubing Xie and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-11-16 with Medical categories.
A thorough overview of nanobiotechnology and its place in advances in applied science and engineering, The Nanobiotechnology Handbook combines contributions from physics, bioorganic and bioinorganic chemistry, molecular and cellular biology, materials science, and medicine as well as from mechanical, electrical, chemical, and biomedical engineering to address the full scope of current and future developments. World-class experts discuss the role of nanobiotechnology in bioanalysis, biomolecular and biomedical nanotechnology, biosensors, biocatalysis and biofuel, and education and workforce development. It includes a companion CD that contains all figures in the book. The book begins with discussions of biomimetic nanotechnology, including a comprehensive overview of DNA nanostructure and DNA-inspired nanotechnology, aptamer-functionalized nanomaterials as artificial antibodies, artificial enzymes, molecular motors, and RNA structures and RNA-inspired nanotechnology. It shows how nanotechnology can be inspired by nature as well as adverse biological events in diagnostic and therapeutic development. From there, the chapters cover major important and widely used nanofabrication techniques, applications of nanotechnology for bioprocessing followed by coverage of the applications of atomic force microscopy (AFM), optical tweezers and nanofluidics as well as other nanotechnology-enabled biomolecular and cellular manipulation and detection. Focusing on major research trends, the book highlights the importance of nanobiotechnology to a range of medical applications such as stem cell technology and tissue engineering, drug development and delivery, imaging, diagnostics, and therapeutics. And with coverage of topics such as nanotoxicity, responsible nanotechnology, and educational and workforce development, it provides a unique overview and perspective of nanobiotechnology impacts from a researcher’s, entrepreneur’s, economist’s and educator’s point of view. It provides a resource for current applications and future development of nanobiotechnology.