Imaging Neurons
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Imaging Neurons
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Author : Rafael Yuste
language : en
Publisher:
Release Date : 2000
Imaging Neurons written by Rafael Yuste and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medical categories.
In the past decade, advances in microscopy have been coupled with new methods of culturing and labeling cells to generate the new science of imaging. Imaging technologies allow investigators to look directly inside living cells and probe their form and function in unprecedented detail. This approach is revolutionizing many aspects of biomedical research, particularly neuroscience, in which visual techniques have traditionally been so important. This manual is the first comprehensive description of the range of imaging technologies being applied to living cells. With its origins in a laboratory course taught at Cold Spring Harbor Laboratory by the editors and contributors, it is packed with the kind of technical detail and practical advice that are essential for success, yet seldom found in the research literature. It covers both established methods and cutting-edge techniques such as multiphoton excitation microscopy and imaging of genetically engineered probes. Although it is neurons to which these technologies are most commonly applied, the methods described are readily adaptable to many other cell types. This book will therefore be an invaluable aid to investigators in cell and developmental biology and immunology as well as neuroscience who wish to take advantage of the extraordinary insights into cellular function offered by imaging technologies.
Membrane Potential Imaging In The Nervous System And Heart
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Author : Marco Canepari
language : en
Publisher: Springer
Release Date : 2015-08-03
Membrane Potential Imaging In The Nervous System And Heart written by Marco Canepari and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-08-03 with Medical categories.
This volume discusses membrane potential imaging in the nervous system and in the heart and modern optical recording technology. Additionally, it covers organic and genetically-encoded voltage-sensitive dyes; membrane potential imaging from individual neurons, brain slices, and brains in vivo; optical imaging of cardiac tissue and arrhythmias; bio-photonics modelling. This is an expanded and fully-updated second edition, reflecting all the recent advances in this field. Twenty chapters, all authored by leading names in the field, are cohesively structured into four sections. The opening section focuses on the history and principles of membrane potential imaging and lends context to the following sections, which examine applications in single neurons, networks, large neuronal populations and the heart. Topics discussed include population membrane potential signals in development of the vertebrate nervous system, use of membrane potential imaging from dendrites and axons, and depth-resolved optical imaging of cardiac activation and repolarization. The final section discusses the potential – and limitations – for new developments in the field, including new technology such as non-linear optics, advanced microscope designs and genetically encoded voltage sensors. Membrane Potential Imaging in the Nervous System and Heart is ideal for neurologists, electro physiologists, cardiologists and those who are interested in the applications and the future of membrane potential imaging.
Imaging In Neuroscience And Development
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Author : Rafael Yuste
language : en
Publisher: CSHL Press
Release Date : 2005
Imaging In Neuroscience And Development written by Rafael Yuste and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Medical categories.
As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well–received Imaging Neurons: A Laboratory Manualhas expanded to include development, with over twenty new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.
Exploration Of Cortical Function
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Author : M. Stetter
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Exploration Of Cortical Function written by M. Stetter and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Exploration of Cortical Function summarizes recent research efforts aiming at the revelation of cortical population coding and signal processing strategies. Topics include optical detection techniques of population activity in the sub-millimeter range, advanced methods for the statistical analysis of these data, and biologically inspired neuronal modeling techniques for population activities in the frameworks of optimal coding, statistical learning theory, and mean-field recurrent networks. Exploration of Cortical Function is unique in that it covers one complete branch of population-based brain research ranging from techniques for data acquisition over data analysis up to modeling techniques for the quantification of functional principles. The volume covers an area which is of great current interest to researchers working on cerebral cortex. The combination of models and image analysis techniques to examine the activity of large cohorts of neurons is especially intriguing and prone to considerable error and debate.
Somatosensory Processing
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Author : Mark Rowe
language : en
Publisher: CRC Press
Release Date : 2003-09-02
Somatosensory Processing written by Mark Rowe and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-09-02 with Science categories.
The diversity of contemporary investigative approaches included in this volume provides an exciting account of our current understanding of brain mechanisms responsible for sensory and perceptual experience in the areas of touch, kinesthesia, and pain. Postgraduate research students in sensory physiology, neurology, psychology and anatomy, and researchers themselves will find that this volume addresses many of the key issues in our attempts to understand the neural mechanisms that mediate sensory experience arising from the body as a whole, the so-called somatic senses, in particular for touch and pain. The volume provides a record of the occasion of the St Petersburg IUPS symposium, chaired by the editors of this volume, and includes some added recent contributions from other leading international figures in the field. Brought together under the sponsoring banner of the IUPS Commission for Somatosensory Physiology and Pain, these scientists with their different experimental approaches seek collectively to understand the brain mechanisms that underlie our own nature and experience.
Advanced Optical Methods For Brain Imaging
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Author : Fu-Jen Kao
language : en
Publisher: Springer
Release Date : 2018-05-14
Advanced Optical Methods For Brain Imaging written by Fu-Jen Kao and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-14 with Technology & Engineering categories.
This book highlights the rapidly developing field of advanced optical methods for structural and functional brain imaging. As is known, the brain is the most poorly understood organ of a living body. It is indeed the most complex structure in the known universe and, thus, mapping of the brain has become one of the most exciting frontlines of contemporary research. Starting from the fundamentals of the brain, neurons and synapses, this book presents a streamlined and focused coverage of the core principles, theoretical and experimental approaches, and state-of-the-art applications of most of the currently used imaging methods in brain research. It presents contributions from international leaders on different photonics-based brain imaging modalities and techniques. Included are comprehensive descriptions of many of the technology driven spectacular advances made over the past few years that have allowed novel insights of the structural and functional details of neurons. The book is targeted at researchers, engineers and scientists who are working in the field of brain imaging, neuroscience and connectomics. Although this book is not intended to serve as a textbook, it will appeal to undergraduate students engaged in the specialization of brain imaging.
Membrane Potential Imaging In The Nervous System
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Author : Marco Canepari
language : en
Publisher: Springer
Release Date : 2017-04-30
Membrane Potential Imaging In The Nervous System written by Marco Canepari and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-04-30 with Medical categories.
The book is structured in five sections, each containing several chapters written by experts and major contributors to particular topics. The volume starts with a historical perspective and fundamental principles of membrane potential imaging and continues to cover the measurement of membrane potential signals from dendrites and axons of individual neurons, measurements of the activity of many neurons with single cell resolution, monitoring of population signals from the nervous system, and concludes with the overview of new approaches to voltage-imaging. The book is targeted at all scientists interested in this mature but also rapidly expanding imaging approach.
In Vivo Optical Imaging Of Brain Function
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Author : Ron D. Frostig
language : en
Publisher: CRC Press
Release Date : 2019-08-30
In Vivo Optical Imaging Of Brain Function written by Ron D. Frostig and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-30 with categories.
These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques and their applications for the growing field of functional imaging in the live brain using optical imaging techniques. New in the Second Edition: Voltage-sensitive dyes imaging in awake behaving animals Imaging based on genetically encoded probes Imaging of mitochondrial auto-fluorescence as a tool for cortical mapping Using pH-sensitive dyes for functional mapping Modulated imaging Calcium imaging of neuronal activity using 2-photon microscopy Fourier approach to optical imaging Fully updated chapters from the first edition Leading Authorities Explore the Latest Techniques Updated to reflect continuous development in this emerging research area, this new edition, as with the original, reaches across disciplines to review a variety of non-invasive optical techniques used to study activity in the living brain. Leading authorities from such diverse areas as biophysics, neuroscience, and cognitive science present a host of perspectives that range from a single neuron to large assemblies of millions of neurons, captured at various temporal and spatial resolutions. Introducing techniques that were not available just a few years ago, the authors describe the theory, setup, analytical methods, and examples that highlight the advantages of each particular method.
Handbook Of Neural Activity Measurement
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Author : Romain Brette
language : en
Publisher: Cambridge University Press
Release Date : 2012-09-06
Handbook Of Neural Activity Measurement written by Romain Brette and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-09-06 with Mathematics categories.
Underlying principles of the various techniques are explained, enabling neuroscientists to extract meaningful information from their measurements.
Imaging And Quantifying Neuronal Autophagy
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Author : Ben Loos
language : en
Publisher: Humana
Release Date : 2021-09-01
Imaging And Quantifying Neuronal Autophagy written by Ben Loos and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-09-01 with Medical categories.
The volume aims to explore the dynamic nature of the autophagy pathway, and the latest techniques that allow researchers to capture and quantify this process in neurons. The chapters in this volume cover topics such as fundamental, historical, and functional approaches that began in baker’s yeast; Saccharomyces cerevisiae; the role of both electron microscopy and live-cell imaging using fluorescently tagged autophagy proteins; and the rate of puncta appearance and its correlation with the rate of autophagosome formation. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Imaging and Quantifying Neuronal is a valuable resource that provides insights into the power of microscopy tools, live-cell imaging, and photoactivation and correlative techniques.