Immunochemical Protocols
DOWNLOAD
Download Immunochemical Protocols PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Immunochemical Protocols book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Immunochemical Protocols
DOWNLOAD
Author : John Pound
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Immunochemical Protocols written by John Pound and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.
Immunochemical Protocols
DOWNLOAD
Author : Margaret M. Manson
language : en
Publisher: Springer
Release Date : 1992
Immunochemical Protocols written by Margaret M. Manson and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Medical categories.
This volume highlights advances in immunochemical techniques and their impact on basic research and clinical medicine. It combines the forces of experts in a wide range of subspecialties to cover a wealth of information.
Plant Virology Protocols
DOWNLOAD
Author : Gary D. Foster
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Plant Virology Protocols written by Gary D. Foster and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.
Human Retrovirus Protocols
DOWNLOAD
Author : Tuofu Zhu
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Human Retrovirus Protocols written by Tuofu Zhu and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.
Flavoprotein Protocols
DOWNLOAD
Author : Steven K. Chapman
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Flavoprotein Protocols written by Steven K. Chapman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.
Basic Methods For The Biochemical Lab
DOWNLOAD
Author : Martin Holtzhauer
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-09-13
Basic Methods For The Biochemical Lab written by Martin Holtzhauer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-09-13 with Science categories.
This book presents proven lab procedures and practical hints for research in analytical and preparative biochemistry, and offers convenient key data in numerous tables. Coverage includes quantitative methods; electrophoresis; chromatographic protocols; immunochemical protocols; centrifugation; and radioactivity. In additional chapters, tables offer quick access to a broad array of useful information, including SI units conversion factors; detergent, protein and nucleotide data; and the basic principles of statistics and enzyme and receptor kinetics are reviewed. This first English-language edition of a successful German-language manual is a valuable resource for students and working professionals in biochemistry, biotechnology and biomedical laboratories.
Pesticide Protocols
DOWNLOAD
Author : José L. Martínez Vidal
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26
Pesticide Protocols written by José L. Martínez Vidal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Science categories.
A comprehensive collection of robust methods for the detection of pesticide compounds or their metabolites useful in food, environmental, and biological monitoring, and in studies of exposure via food, water, air, and the skin or lungs. The readily reproducible methods range from gas and liquid chromatography coupled to mass spectrometry detection and other classic detectors, to capillary electrophoresis and immunochemical or radioimmunoassay methods. The authors have focused on extraction and cleanup procedures, in order to develop and optimize more fullyautomated and miniaturized methods, including solid-phase extraction, solid-phase microextraction, microwave-assisted extraction, and on-line tandem liquid chromatography (LC/LC) trace enrichment, among others. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Immunochemistry
DOWNLOAD
Author : Vanoss
language : en
Publisher: CRC Press
Release Date : 1994-08-24
Immunochemistry written by Vanoss and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-08-24 with Medical categories.
This work offers comprehensive coverage of the chemical and physicochemical aspects of immunological interactions, as well as the molecules and moieties involved in these interactions. It covers in detail the Ag-Ab interaction, including attraction at a distance between epitope and paratope. College or university bookstores may order five or more copies at a special student price, available upon request.
Receptor Binding Techniques
DOWNLOAD
Author : Anthony P. Davenport
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Receptor Binding Techniques written by Anthony P. Davenport and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
A comprehensive collection of readily reproducible methods for studying receptors in silico, in vitro, and in vivo. These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.
Oligonucleotide Synthesis
DOWNLOAD
Author : Piet Herdewijn
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Oligonucleotide Synthesis written by Piet Herdewijn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Medical categories.
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.