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Immunochemical Protocols


Immunochemical Protocols
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Immunochemical Protocols


Immunochemical Protocols
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Author : John Pound
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03

Immunochemical Protocols written by John Pound and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.


This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.



Immunochemical Protocols Methods In Molecular Biology


Immunochemical Protocols Methods In Molecular Biology
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Author : John Pound
language : en
Publisher:
Release Date : 1998

Immunochemical Protocols Methods In Molecular Biology written by John Pound and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with categories.


This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including conventional" and recombinant antibodies, fusion proteins, and their various conjugates. The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities."



Immunohistochemistry And Immunocytochemistry


Immunohistochemistry And Immunocytochemistry
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Author : Simon Renshaw
language : en
Publisher: John Wiley & Sons
Release Date : 2017-02-06

Immunohistochemistry And Immunocytochemistry written by Simon Renshaw and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-02-06 with Science categories.


Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.



Immunochemical Techniques Laboratory Manual


Immunochemical Techniques Laboratory Manual
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Author : John Goers
language : en
Publisher:
Release Date : 1993

Immunochemical Techniques Laboratory Manual written by John Goers and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Medical categories.


This is a laboratory manual for a course in immunochemical techniques designed to introduce students to the subject, enabling them to understand the wider applications of the techniques they are using. The text is written with easy-to-follow protocols for immunological techniques used in modern research labs and biochemical companies.



Immunochemical Protocols


Immunochemical Protocols
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Author : Margaret M. Manson
language : en
Publisher: Springer
Release Date : 1992

Immunochemical Protocols written by Margaret M. Manson and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Medical categories.


This volume highlights advances in immunochemical techniques and their impact on basic research and clinical medicine. It combines the forces of experts in a wide range of subspecialties to cover a wealth of information.



The Protein Protocols Handbook


The Protein Protocols Handbook
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Author : John M. Walker
language : en
Publisher: Springer
Release Date : 2007-10-02

The Protein Protocols Handbook written by John M. Walker and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-02 with Science categories.


The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.



Pesticide Protocols


Pesticide Protocols
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Author : José L. Martínez Vidal
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26

Pesticide Protocols written by José L. Martínez Vidal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Science categories.


A comprehensive collection of robust methods for the detection of pesticide compounds or their metabolites useful in food, environmental, and biological monitoring, and in studies of exposure via food, water, air, and the skin or lungs. The readily reproducible methods range from gas and liquid chromatography coupled to mass spectrometry detection and other classic detectors, to capillary electrophoresis and immunochemical or radioimmunoassay methods. The authors have focused on extraction and cleanup procedures, in order to develop and optimize more fullyautomated and miniaturized methods, including solid-phase extraction, solid-phase microextraction, microwave-assisted extraction, and on-line tandem liquid chromatography (LC/LC) trace enrichment, among others. The protocols offer step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.



Immunochemical Methods In Cell And Molecular Biology


Immunochemical Methods In Cell And Molecular Biology
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Author :
language : en
Publisher: Academic Press
Release Date : 1987

Immunochemical Methods In Cell And Molecular Biology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Science categories.


This book presents up-to-date immunochemical techniques for isolating and analyzing a wide variety of biological products. It collates existing information in one source, presenting important basic and applied advances and provides a stimulating forum for the discussion of new ideas and observations on genetically manipulated microbial and viral agents.



Immunochemistry Of Solid Phase Immunoassay


Immunochemistry Of Solid Phase Immunoassay
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Author : John E. Butler
language : en
Publisher: CRC Press
Release Date : 1991-07-23

Immunochemistry Of Solid Phase Immunoassay written by John E. Butler and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-07-23 with Science categories.


Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.



Environmental Immunochemical Analysis Detection Of Pesticides And Other Chemicals


Environmental Immunochemical Analysis Detection Of Pesticides And Other Chemicals
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Author : Shirley J. Gee
language : en
Publisher: Cambridge University Press
Release Date : 2008-10-19

Environmental Immunochemical Analysis Detection Of Pesticides And Other Chemicals written by Shirley J. Gee and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-10-19 with Science categories.


This book is a tutorial designed to instruct the reader in use and application of immunochemical methods of analysis for environmental contaminants. A brief introduction describes basic principles and the advantages and disadvantages of the technology, and gives a listing of references which supply more detail. Preparation of the laboratory for use of this technology and the general scientific considerations prior to using the technology are discussed. Detailed step-wise procedures are given for analysis of selected analytes, triazine herbicides, carbaryl, paraquat, and p-nitrophenols, etc. In addition to the specific immunoassay methods, a series of support techniques necessary to perform immunochemical methods are described. This book provides specific instruction for certain analytes, but also serves to familiarize the novice reader with many generic concepts needed to successfully utilize immunochemistry technology including: applications, sampling, sample preparation, extraction, cleanup, quality assurance, methods development and optimization, data handling and troubleshooting. It is not necessary for the reader to actually perform the immunoassays given in this user's guide to obtain familiarity with these concepts. The guide is written so that the information presented can be applied to other immunoassays not given here. Thus, the strength of the guide is its universal applicability to immunoassay methods.