In Vivo Fluorescence Imaging Success In Preclinical Imaging Paves The Way For Clinical Applications
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In Vivo Fluorescence Imaging Success In Preclinical Imaging Paves The Way For Clinical Applications
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Author : Ahmed Refaat
language : en
Publisher:
Release Date : 2022
In Vivo Fluorescence Imaging Success In Preclinical Imaging Paves The Way For Clinical Applications written by Ahmed Refaat and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with categories.
Abstract: Advances in diagnostic imaging have provided unprecedented opportunities to detect diseases at early stages and with high reliability. Diagnostic imaging is also crucial to monitoring the progress or remission of disease and thus is often the central basis of therapeutic decision-making. Currently, several diagnostic imaging modalities (computed tomography, magnetic resonance imaging, and positron emission tomography, among others) are routinely used in clinics and present their own advantages and limitations. In vivo near-infrared (NIR) fluorescence imaging has recently emerged as an attractive imaging modality combining low cost, high sensitivity, and relative safety. As a preclinical tool, it can be used to investigate disease mechanisms and for testing novel diagnostics and therapeutics prior to their clinical use. However, the limited depth of tissue penetration is a major challenge to efficient clinical use. Therefore, the current clinical use of fluorescence imaging is limited to a few applications such as image-guided surgery on tumors and retinal angiography, using FDA-approved dyes. Progress in fluorophore development and NIR imaging technologies holds promise to extend their clinical application to oncology, cardiovascular diseases, plastic surgery, and brain imaging, among others. Nanotechnology is expected to revolutionize diagnostic in vivo fluorescence imaging through targeted delivery of NIR fluorescent probes using antibody conjugation. In this review, we discuss the latest advances in in vivo fluorescence imaging technologies, NIR fluorescent probes, and current and future clinical applications
In Vivo Fluorescence Imaging Methods And Protocols
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Author : Mingfeng Bai
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-06-24
In Vivo Fluorescence Imaging Methods And Protocols written by Mingfeng Bai and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-06-24 with Medical categories.
This detailed volume includes a rich variety of applications using various instrumentations, probes, disease models, and targets in order to account for the multidisciplinary nature of the use of in vivo fluorescence imagine. The book also includes chapters on the emerging fields of cell tracking, image-guided treatment, and fluorescence imaging in the second NIR window, as well as protocols for evaluation methods before and after in vivo imaging. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Vivo Fluorescence Imaging: Methods and Protocols serves as a valuable reference for researchers from numerous fields who wish to become more familiar with in vivo fluorescence imaging techniques.
Mouse Models Of Cancer
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Author : Maja Čemažar
language : en
Publisher: Springer Nature
Release Date : 2024-02-19
Mouse Models Of Cancer written by Maja Čemažar and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-02-19 with Medical categories.
This detailed volume presents a variety of mouse models for cancer studies, from leukemia and lymphoma models to different types of subcutaneous and orthotopic models. Models for the healing process and the assessment of the immune response after local ablative therapies are also included, as well as imaging techniques that allow for the visualization of cancer at the cellular and tissue level. The book closes with a detailed description of necropsy, which is essential for obtaining good biomaterial for translational research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mouse Models of Cancer: Methods and Protocols serves as an ideal guide to these controlled and reproducible model experimental systems to study different aspects of cancer biology, including prevention, development, progression, and treatment.
Development And Applications Of Novel Fluorescent Molecular Probe Strategies
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Author : Dolonchampa Maji
language : en
Publisher:
Release Date : 2017
Development And Applications Of Novel Fluorescent Molecular Probe Strategies written by Dolonchampa Maji and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with Electronic dissertations categories.
Optical imaging and spectroscopy technologies offer the ability to provide structural and functional information in a fast, low-cost, ionizing radiation free, highly sensitive and high throughput fashion. The diverse contrast mechanisms and complementary imaging platforms form the foundation for the application of optical imaging in pre-clinical studies of pathophysiological development as well as direct clinical application as a tool for diagnosis and therapy. Fluorescence imaging techniques have been one of the most rapidly adopted methods in biology and biomedicine. Visualization of biological processes and pathologic conditions at the cellular and tissue levels largely relies on the use of exogenous fluorophores or their bioconjugates. Some fluorescent molecular probes provide usable contrast for disease diagnosis due to their responsiveness to interactions with other molecular species and/or immediate microenvironment. As a result, understanding exogenous fluorescent contrast mechanisms will allow the development of efficient strategies for biomedical fluorescence imaging. The present work focuses on exploring novel fluorescent molecular probe strategies for imaging cancer and cardiovascular diseases. We have developed a platform for synthesizing activatable fluorescent molecular probes using the fluorescence quenching properties of copper (II) ions. We used these activatable probes for rapid imaging of cancerous tissue in vivo in mice. While developing these molecular probes, we discovered an unexpected molecular interaction that yields stable dimeric molecules. This finding can potentially enable the development of new molecular entities for modifying the signaling properties of fluorescent dyes to minimize background fluorescence. Although planar fluorescence imaging methods using exogenous molecular probes provide rapid information about molecular processes in vivo, extraction of depth information require complex data acquisition and image analysis methods. By designing a dual emission fluorescent probe incorporating two spectrally different fluorophore systems, we developed a method to successfully estimate the depth of fluorescent inclusions from planar imaging data and demonstrated the potential of using this approach to locate a blood vessel and tumorous tissue in mouse in vivo. An important feature of fluorescence methods is the availability of various techniques that provide complementary information. Combining the fluorescence intensity and lifetime properties of a biologically targeted near infrared fluorescent probe, we demonstrate an effective way to distinguish specific from nonspecific uptake mechanisms in cancer cells, an approach that can be translated in vivo. Alternatively, dynamic fluorescence imaging technique expands the scope of applications to include detection and estimation of the size of circulating cancer cells and clusters. The approach developed in this work could allow longitudinal monitoring of these cells, which are implicated in cancer metastases. To circumvent the shallow penetration of light using optical methods, we developed multimodal imaging approaches by incorporating a radionuclide for nuclear imaging into a broad spectrum near infrared fluorescent tumor targeting agent. This molecular construct allows for noninvasive whole body nuclear imaging of tumors, followed by fluorescence image guided resection. In each of these areas, novel fluorescent molecular probes were developed, characterized and applied to solve critical biomedical problems.
Optical Methods For Managing The Diabetic Foot
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Author : Gennadi Saiko
language : en
Publisher: CRC Press
Release Date : 2023-12-11
Optical Methods For Managing The Diabetic Foot written by Gennadi Saiko and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-12-11 with Science categories.
This book discusses optical technologies for Diabetic Foot management. It combines the current medical literature review with an overview of the technology and physics behind it. Thus, it is a single-source introduction to the topic. It can also be used as a reference source and practical guide for the use of technology. The particular focus is on low-cost technologies, including hyperspectral imaging, thermography, and endogenous bacterial fluorescence. Moving diagnostic modalities closer to the patient (e.g., primary care) allows the disease to be detected at an earlier stage, thus improving outcomes. However, while some optical technologies are available commercially, they have not received wide clinical adoption due to gaps in knowledge translation to mainstream medicine. This book aims to narrow this gap with practical illustrations. The book will be of interest to a broad range of healthcare professionals, clinical researchers, engineers, and decision-makers, who are dealing with complications of diabetes. Key Features: Reviews the current state of technologies. Provides a practical guide with practical considerations and illustrations. Supplies a 360-degree view of the combination of clinical information with a technology background and primers on physics and engineering.
A New Modality For High Resolution Diffuse Optical Imaging
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Author : Tiffany C. Kwong
language : en
Publisher:
Release Date : 2017
A New Modality For High Resolution Diffuse Optical Imaging written by Tiffany C. Kwong and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
As one of the most sensitive in vivo molecular imaging modalities, fluorescence imaging has great potential to play an important role in preclinical and clinical studies. Indeed, in vivo fluorescence optical imaging extends across a wide range of applications, from cellular to organ levels. Unfortunately, in organ level applications, fluorescence imaging suffers from low spatial resolution due to the high scattering nature of biological tissue, especially in deep tissue (>1cm). Extensive effort has been spent to improve the spatial resolution of fluorescence tomography (FT). However, approaches such as integrating FT with other anatomic imaging modalities such as Magnetic Resonance Imaging (MRI) or computed tomography (CT) do not perform well if the fluorescent agent distribution within the medium cannot be defined in the anatomical image. There are also several techniques that attempt to modulate fluorescence signals using ultrasound to achieve higher spatial resolution. However, low modulation efficiency and extremely low signal to noise ratio (SNR) are the two primary factors that make the implementation of these techniques difficult.Consequently, the poor spatial resolution and subsequent low quantitative accuracy are the main obstacles preventing the widespread use of this powerful technique in pre-clinical and clinical settings. To overcome these limitations, the goal of this thesis is to develop an entirely new approach termed, "Temperature-modulated fluorescence tomography (TM-FT)", that can provide high resolution images at depths up to 6 cm without sacrificing the exceptional sensitivity of FT. In this innovative approach, FT is combined with temperature activatable fluorescence molecular probes (ThermoDots) and high intensity focused ultrasound (HIFU) which can provide localized heating of the tissue (only 3-5 °C) with high spatial resolution. The small size of the focal spot (~1.4 mm) allows imaging the distribution of these temperature sensitive agents with not only high spatial resolution but also high quantitative accuracy.This thesis will present the development of the first TM-FT system prototype including the instrumentation and system design, image reconstruction algorithm, and ThermoDots probe development. The feasibility of this method to provide superior spatial resolution and high quantitative accuracy is validated using simulations and experimentally demonstrated in phantom and ex vivo tissue.
Nanotechnology Enabled Biomedical Fluorescence Imaging In The Second Near Infrared Window
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Author : Guosong Hong
language : en
Publisher:
Release Date : 2014
Nanotechnology Enabled Biomedical Fluorescence Imaging In The Second Near Infrared Window written by Guosong Hong and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
Fluorescence imaging in the second near-infrared window (NIR-II, 1.0-1.7 microns) has many salient advantages over the visible (400-750 nm) and the traditional near-infrared (NIR-I, 750-900 nm) windows owing to the reduced photon scattering and negligible tissue autofluorescence. However, NIR-II fluorescence imaging has been limited by the scarcity of materials with sufficient NIR-II fluorescence quantum efficiency, and single-walled carbon nanotube (SWNT) had been the only fluorophore for biological imaging in the NIR-II window. This work aims to enhance the intrinsic NIR-II fluorescence of SWNTs, apply SWNTs for in vivo imaging of real-world medical problems in animal models and develop new NIR-II fluorophores other than SWNTs. First, a plasmonic gold substrate is used to enhance the intrinsically low NIR-II fluorescence of SWNTs and to improve the sensitivity of cancer cell imaging using SWNTs as molecular targeting probes. The sensitive distance dependence of fluorescence enhancement of SWNTs is then exploited to probe the trans-membrane motion of single nanotube molecules and reveal the internalization pathway as receptor-mediated endocytosis. The biocompatible SWNTs are further applied to an in vivo animal model of lower limb ischemia, where we demonstrate microvascular imaging and hemodynamic measurement using NIR-II fluorescence, with improved spatial resolution over X-ray computer tomography (CT) and broader dynamic range of blood flowmetry than ultrasound. In a rationally chosen sub-region of NIR-II in the 1.3-1.4 micron range, chemically separated SWNTs allow for non-invasive brain vascular imaging through intact scalp and skull with sub-10 micron resolution at millimeter depth of penetration. Lastly, two new materials, Ag2S quantum dots (QDs) and conjugated copolymers are developed to expand the toolbox of NIR-II fluorophores. The Ag2S QDs afford in vitro targeted cancer cell imaging and in vivo mouse imaging with high tumor uptake. The high fluorescence quantum yield of the conjugated copolymer allows for ultrafast dynamic NIR-II imaging of the arterial blood flow with waveform cardiac cycles revealed in hemodynamic analysis. The many benefits of NIR-II fluorescence imaging demonstrated in this work based on the development of a handful of biocompatible NIR-II nanomaterials bode well for future biological research and clinical applications with this new imaging technique.
Multi Modality Diffuse Fluorescence Imaging Applied To Preclinical Imaging In Mice
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Author : Baoqiang Li
language : en
Publisher:
Release Date : 2014
Multi Modality Diffuse Fluorescence Imaging Applied To Preclinical Imaging In Mice written by Baoqiang Li and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
Overall this thesis was dedicated to multi-modality targeted fluorescence imaging in mice. Results from the three articles evaluated the feasibility and performance of the combined imaging approach in simulations, phantoms and mice in vivo. Results suggested that multi-modality fluorescence imaging might serve as a potent tool for preclinical and biological study in mice.
Fundamentals Of Fluorescence Imaging
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Author : Guy Cox
language : en
Publisher: CRC Press
Release Date : 2019-04-23
Fundamentals Of Fluorescence Imaging written by Guy Cox and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-23 with Science categories.
Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism. This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.
Nanomaterials And Their Biomedical Applications
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Author : Tuhin Subhra Santra
language : en
Publisher: Springer Nature
Release Date : 2021-04-16
Nanomaterials And Their Biomedical Applications written by Tuhin Subhra Santra and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-04-16 with Science categories.
This book highlights the evolution of, and novel challenges currently facing, nanomaterials science, nanoengineering, and nanotechnology, and their applications and development in the biological and biomedical fields. It details different nanoscale and nanostructured materials syntheses, processing, characterization, and applications, and considers improvements that can be made in nanostructured materials with their different biomedical applications. The book also briefly covers the state of the art of different nanomaterials design, synthesis, fabrication and their potential biomedical applications. It will be particularly useful for reading and research purposes, especially for science and engineering students, academics, and industrial researchers.