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Integrated Methods In Protein Biochemistry Part A


Integrated Methods In Protein Biochemistry Part A
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Integrated Methods In Protein Biochemistry Part A


Integrated Methods In Protein Biochemistry Part A
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Author :
language : en
Publisher: Elsevier
Release Date : 2022-10-09

Integrated Methods In Protein Biochemistry Part A written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-10-09 with Science categories.


Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more. Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Updated release includes the latest information on Integrated Methods in Protein Biochemistry



Integrated Methods In Protein Biochemistry Part A


Integrated Methods In Protein Biochemistry Part A
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Author :
language : en
Publisher: Academic Press
Release Date : 2022-10-10

Integrated Methods In Protein Biochemistry Part A written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-10-10 with Science categories.


Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more. Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.



Integrated Methods In Protein Biochemistry Part B


Integrated Methods In Protein Biochemistry Part B
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Author :
language : en
Publisher: Elsevier
Release Date : 2023-01-20

Integrated Methods In Protein Biochemistry Part B written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-20 with Science categories.


Integrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Precise modification of native proteins, purification, and analysis of bioconjugates, NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling, Structure and function of bacterial secretion system, Tools and protocols for probing protein sumoylation, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more. Additional sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Structural and Functional characterization of lytic polysaccharide monooxygenases, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology series - Includes the latest information on Integrated Methods in Protein Biochemistry



Integrated Methods In Protein Biochemistry Part C


Integrated Methods In Protein Biochemistry Part C
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Author : Arun K. Shukla
language : en
Publisher: Elsevier
Release Date : 2023-03-20

Integrated Methods In Protein Biochemistry Part C written by Arun K. Shukla and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-03-20 with Science categories.


Integrated Methods in Protein Biochemistry: Part C, Volume 679 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling, Biochemical Analysis of Protein-Protein Interfaces underlying the regulation of Bacterial Secretion Systems, Probing the structure and function of N-acetylmannosamine-6-phosphate 2-epimerase, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more. Other sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Functional characterization of lytic polysaccharide monooxygenases (LPMOs), Characterization of RRE domain in RiPP biosynthesis, The Preparation of Recombinant Arginyltransferase 1 (ATE1) for Structural and Biophyiscal Characterizations, Testing anti-cancer drugs with Holographic Incoherent-light-source Quantitative Phase Imaging, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in Methods in Enzymology serials - Updated release includes the latest information on Integrated Methods in Protein Biochemistry



Integrated Methods In Protein Biochemistry Part B


Integrated Methods In Protein Biochemistry Part B
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Author :
language : en
Publisher: Academic Press
Release Date : 2023-01-18

Integrated Methods In Protein Biochemistry Part B written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-18 with Science categories.


Integrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Precise modification of native proteins, purification, and analysis of bioconjugates, NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein ß? subunit and PLC-ß enzyme in PIP2 hydrolysis and downstream signaling, Structure and function of bacterial secretion system, Tools and protocols for probing protein sumoylation, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more. Additional sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Structural and Functional characterization of lytic polysaccharide monooxygenases, and much more.



Laboratory Methodology In Biochemistry


Laboratory Methodology In Biochemistry
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Author : C. Fini
language : en
Publisher: CRC Press
Release Date : 2019-08-08

Laboratory Methodology In Biochemistry written by C. Fini and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-08 with Medical categories.


Biochemistry Physical Biochemistry Molecular Biology Clinical Biochemistry Biochemical Technology Applied Biochemistry Cellular Biology



Plant Analysis Comprehensive Methods And Protocols


Plant Analysis Comprehensive Methods And Protocols
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Author : B.K. Garg
language : en
Publisher: Scientific Publishers
Release Date : 2012-06-01

Plant Analysis Comprehensive Methods And Protocols written by B.K. Garg and has been published by Scientific Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-01 with Technology & Engineering categories.


The book `Plant Analysis: Comprehensive Methods and Protocols' is a complete laboratory manual for analytical methods and techniques in the field of Agriculture, Plant Physiology, Biochemistry and related Plant Sciences. Right from nutrient analysis in plants, it covers estimations of macromolecules, such as amino acids, proteins, nucleic acids and metabolites of fatty acid metabolism. Protocols for the assay of various enzymes of nitrogen metabolism, ammonia assimilation, photosynthetic CO2-fixation, reactive oxygen species, carbohydrate, phosphorus and energy metabolism have been elucidated in the book. Special emphasis has also been given to techniques on specific topics such as Electrophoresis, Molecular Biology, Histo-enzymology, Symbiotic Nitrogen Fixation and assay of plant growth hormones. Thus the present book is one stop solution for all important techniques and analytical methods for students and research workers engaged in plant sciences and agricultural research.



Protein Structural Biology In Biomedical Research Part A


Protein Structural Biology In Biomedical Research Part A
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Author : C. Woodward
language : en
Publisher: Elsevier
Release Date : 1998-01-16

Protein Structural Biology In Biomedical Research Part A written by C. Woodward and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-01-16 with Science categories.


Recent advances in protein structural biology, coupled with new developments in human genetics, have opened the door to understanding the molecular basis of many metabolic, physiological, and developmental processes in human biology. Medical pathologies, and their chemical therapies, are increasingly being described at the molecular level. For single-gene diseases, and some multi-gene conditions, identification of highly correlated genes immediately leads to identification of covalent structures of the actual chemical agents of the disease, namely the protein gene products. Once the primary sequence of a protein is ascertained, structural biologists work to determine its three-dimensional, biologically active structure, or to predict its probable fold and/or function by comparison to the data base of known protein structures. Similarly, three-dimensional structures of proteins produced by microbiological pathogens are the subject of intense study, for example, the proteins necessary for maturation of the human HIV virus. Once the three-dimensional structure of a protein is known or predicted, its function, as well as potential binding sites for drugs that inhibit its function, become tractable questions. The medical ramifications of the burgeoning results of protein structural biology, from gene replacement therapy to "rational" drug design, are well recognized by researchers in biomedical areas, and by a significant proportion of the general population. The purpose of this book is to introduce biomedical scientists to important areas of protein structural biology, and to provide an insightful orientation to the primary literature that shapes the field in each subject. The chapters in this volume cover aspects of protein structural biology which have led to the recognition of fundamental relationships between protein structure and function.



Small Angle Scattering Part A Methods For Structural Investigation


Small Angle Scattering Part A Methods For Structural Investigation
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Author :
language : en
Publisher: Academic Press
Release Date : 2022-11-19

Small Angle Scattering Part A Methods For Structural Investigation written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-19 with Science categories.


Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications. Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Enzymology series - Includes the latest information on Small Angle Scattering: Methods for Structural Investigation



Comprehensive Biomaterials Ii


Comprehensive Biomaterials Ii
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Author : Kevin Healy
language : en
Publisher: Elsevier
Release Date : 2017-05-18

Comprehensive Biomaterials Ii written by Kevin Healy and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-05-18 with Technology & Engineering categories.


Comprehensive Biomaterials II, Second Edition, Seven Volume Set brings together the myriad facets of biomaterials into one expertly-written series of edited volumes. Articles address the current status of nearly all biomaterials in the field, their strengths and weaknesses, their future prospects, appropriate analytical methods and testing, device applications and performance, emerging candidate materials as competitors and disruptive technologies, research and development, regulatory management, commercial aspects, and applications, including medical applications. Detailed coverage is given to both new and emerging areas and the latest research in more traditional areas of the field. Particular attention is given to those areas in which major recent developments have taken place. This new edition, with 75% new or updated articles, will provide biomedical scientists in industry, government, academia, and research organizations with an accurate perspective on the field in a manner that is both accessible and thorough. Reviews the current status of nearly all biomaterials in the field by analyzing their strengths and weaknesses, performance, and future prospects Covers all significant emerging technologies in areas such as 3D printing of tissues, organs and scaffolds, cell encapsulation; multimodal delivery, cancer/vaccine - biomaterial applications, neural interface understanding, materials used for in situ imaging, and infection prevention and treatment Effectively describes the many modern aspects of biomaterials from basic science, to clinical applications