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Introduction To Optical Microscopy


Introduction To Optical Microscopy
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Introduction To Optical Microscopy


Introduction To Optical Microscopy
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Author : Jerome Mertz
language : en
Publisher: Cambridge University Press
Release Date : 2019-08

Introduction To Optical Microscopy written by Jerome Mertz and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08 with Medical categories.


Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.



Introduction To Light Microscopy


Introduction To Light Microscopy
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Author : Dee Lawlor
language : en
Publisher: Springer
Release Date : 2019-05-31

Introduction To Light Microscopy written by Dee Lawlor and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-31 with Science categories.


This book offers a beginner’s guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section also helps resolve some of the most common issues encountered in light microscopy. Most people have a general idea of how to use a microscope, but many never get the full benefit, because they receive no training. With easy-to-follow steps and detailed images, this guide will help everyone achieve the best results, and be confident using their microscope. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists, educators who teach science to young children, and anyonewith a general interest in these valuable tools.



A Practical Guide To Optical Microscopy


A Practical Guide To Optical Microscopy
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Author : John Girkin
language : en
Publisher: CRC Press
Release Date : 2019-06-14

A Practical Guide To Optical Microscopy written by John Girkin and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-14 with Science categories.


Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.



Understanding Light Microscopy


Understanding Light Microscopy
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Author : Jeremy Sanderson
language : en
Publisher: John Wiley & Sons
Release Date : 2019-05-06

Understanding Light Microscopy written by Jeremy Sanderson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-06 with Science categories.


Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.



Optical Imaging And Microscopy


Optical Imaging And Microscopy
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Author : Peter Török
language : en
Publisher: Springer Science & Business Media
Release Date : 2003

Optical Imaging And Microscopy written by Peter Török and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with Medical categories.


Annotation This text on contemporary optical systems is intended for optical researchers and engineers, graduate students and optical microscopists in the biological and biomedical sciences. In three sections, the book discusses high-aperture optical systems, nonlinear optical techniques, and various techniques that are finding new applications. The new second edition has been thoroughly revised and expanded to account for new advances in fluorescence imaging and diffractive optical lenses.



Introduction To Confocal Fluorescence Microscopy


Introduction To Confocal Fluorescence Microscopy
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Author : Michiel Muller
language : en
Publisher: SPIE Press
Release Date : 2006

Introduction To Confocal Fluorescence Microscopy written by Michiel Muller and has been published by SPIE Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Science categories.


This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.



Confocal Scanning Optical Microscopy And Related Imaging Systems


Confocal Scanning Optical Microscopy And Related Imaging Systems
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Author : Gordon S. Kino
language : en
Publisher: Academic Press
Release Date : 1996-09-18

Confocal Scanning Optical Microscopy And Related Imaging Systems written by Gordon S. Kino and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-09-18 with Science categories.


This book provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers. The book concentrates mainly on two instruments: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A comprehensive discussion of the theory and design of the Near-Field Scanning Optical Microscope (NSOM) is also given. The text discusses the practical aspects of building a confocal scanning optical microscope or optical interference microscope, and the applications of these microscopes to phase imaging, biological imaging, and semiconductor inspection and metrology.A comprehensive theoretical discussion of the depth and transverse resolution is given with emphasis placed on the practical results of the theoretical calculations and how these can be used to help understand the operation of these microscopes. - Provides a comprehensive introduction to the field of scanning optical microscopy for scientists and engineers - Explains many practical applications of scanning optical and interference microscopy in such diverse fields as biology and semiconductor metrology - Discusses in theoretical terms the origin of the improved depth and transverse resolution of scanning optical and interference microscopes with emphasis on the practical results of the theoretical calculations - Considers the practical aspects of building a confocal scanning or interference microscope and explores some of the design tradeoffs made for microscopes used in various applications - Discusses the theory and design of near-field optical microscopes - Explains phase imaging in the scanning optical and interference microscopes



Optical Design Of Microscopes


Optical Design Of Microscopes
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Author : George Seward
language : en
Publisher: Society of Photo Optical
Release Date : 2009-07-15

Optical Design Of Microscopes written by George Seward and has been published by Society of Photo Optical this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-07-15 with Science categories.


Knowledge of microscope design is rapidly becoming more important. Microscopes are used in critical applications such as drug development, clinical tests, and genomics. Considerable expertise is required for the evaluation, design, and manufacture of these instruments. Several subsystems must be integrated: the source, the illumination optics, the specimen, the objective lens, the tube optics, and the sensor. The large numerical aperture of a microscope is essential for small spot size and high brightness; however, the large numerical aperture also presents difficult issues in optical design and fabrication. This book provides a foundation for developing design expertise through education, practice, and exploration. It is suitable for lens designers, optical engineers, and students with a basic knowledge of microscope structure.



Optical Fluorescence Microscopy


Optical Fluorescence Microscopy
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Author : Alberto Diaspro
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-11-25

Optical Fluorescence Microscopy written by Alberto Diaspro and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-25 with Science categories.


In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.