Isolation Of Plant Organelles And Structures Methods And Protocols
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Isolation Of Plant Organelles And Structures Methods And Protocols
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Author : Nicolas L. Taylor
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-10-26
Isolation Of Plant Organelles And Structures Methods And Protocols written by Nicolas L. Taylor and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-26 with Science categories.
The Plant Cell Wall
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Author : Zoë A. Popper
language : en
Publisher: Humana
Release Date : 2020-07-03
The Plant Cell Wall written by Zoë A. Popper and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-03 with Science categories.
This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and structure, plant tissue culture protoplast isolation, genetic manipulation, investigation of enzyme activities, and in situ localization of wall components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Plant Cell Wall: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists interested in learning more about this field.
Subcellular Fractionation
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Author : Paul R. Pryor
language : en
Publisher:
Release Date : 2015
Subcellular Fractionation written by Paul R. Pryor and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with Cell fractionation categories.
Eukaryotic cells are remarkably complex structures, containing a vast repertoire of macromolecules, organelles, and other compartments that orchestrate the tasks required for life. For in-depth studies of their function and composition, reliable methods for the isolation of specific subcellular structures are often required. This laboratory manual provides step-by-step protocols for the extraction of subcellular components from animal tissues, yeasts, plants, and cultured cells. Each chapter focuses on a particular eukaryotic organelle, vesicle, membrane, or macromolecular complex. Strategies for breaking cells while maintaining the structural and functional integrity of the component of interest, enriching for that component based on its physical and biochemical characteristics, and monitoring and ensuring the success of the purification procedure are provided. The contributors describe both traditional approaches (e.g., density gradient centrifugation) and innovative techniques (e.g., the use of SPIONs) for isolating subcellular constituents. This manual is therefore an essential laboratory resource for all cell biologists seeking a comprehensive collection of dependable subcellular fractionation methods.
The Plant Cell Wall Methods And Protocols
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Author : Zoë A. Popper
language : en
Publisher: Lulu.com
Release Date :
The Plant Cell Wall Methods And Protocols written by Zoë A. Popper and has been published by Lulu.com this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Rna Isolation And Characterization Protocols
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Author : Ralph Rapley
language : en
Publisher: Humana Press
Release Date : 2013-08-18
Rna Isolation And Characterization Protocols written by Ralph Rapley and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-08-18 with Science categories.
Ribonucleic acids are central to cellular and molecular processes and perform vital functions in both structural and functional roles. RNA molecules form the bridge between the stable genetic information contained within DNA and enzymes and proteins that carry out much of the metabolism within the cell. Many of the sites of protein synthesis, the ribosomes within the cell, are composed of these ribonucleic acids as are the tRNA molecules that deliver the amino acid building blocks to the ribosomes. Of all the RNA species, the nucleic acid intermediate, messenger RNA, is a desirable source of material to biologists, since this reflects much of, what ultimately, is translated into enzymes and proteins. In order to determine the qualitative and quantitative changes in mRNA expression, a vast number of molecular biological techniques have been developed. Key molecular methods that provide the means to initially isolate and analyze RNA molecules are the focus of this volume. In putting together this collection of protocols, we have tried to provide techniques that are most applicable and widely used. In particular, there are a number of iso- tion techniques included that have been developed, modified, or adapted to enable extraction from a variety of cell types, organisms, or subcellular organelles. Successful isolation of intact RNA is an essential starting point for any sub- quent analysis. This is why we have aimed to make this section comprehensive. The analysis of RNA is the focus of the following chapters.
Sub Cellular Proteomics
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Author : Harvey Millar
language : en
Publisher: Frontiers E-books
Release Date : 2015-01-05
Sub Cellular Proteomics written by Harvey Millar and has been published by Frontiers E-books this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-01-05 with Botany categories.
Whilst significant advances have been made in whole organismal proteomics approaches, many researchers still rely on combinations of tissue selection and subcellular prefractionation methods to reduce the complexity of protein extracts from plants prior to proteomic analysis. Often this will allow identification of many lower abundance proteins of the target proteome and it may involve the selection of specific organs, cell types or the isolation of specific subcellular components. These subcellular proteomes provide insight into functions following various treatments and also contribute to the wider understanding of the entire organismal proteome by cataloguing a series of sub-proteome contents. The aim of this Research Topic is to bring together knowledge of sub cellular components in different plant species to provide a basis for accelerated research. It aims to provide a mini-review for each proposed section that summarizes the current understanding of a particular proteome, with the anticipation that every 5 - 10 years we can update these definitive publications.
Plant Analysis Comprehensive Methods And Protocols
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Author : B.K. Garg
language : en
Publisher: Scientific Publishers
Release Date : 2012-06-01
Plant Analysis Comprehensive Methods And Protocols written by B.K. Garg and has been published by Scientific Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-01 with Technology & Engineering categories.
The book `Plant Analysis: Comprehensive Methods and Protocols' is a complete laboratory manual for analytical methods and techniques in the field of Agriculture, Plant Physiology, Biochemistry and related Plant Sciences. Right from nutrient analysis in plants, it covers estimations of macromolecules, such as amino acids, proteins, nucleic acids and metabolites of fatty acid metabolism. Protocols for the assay of various enzymes of nitrogen metabolism, ammonia assimilation, photosynthetic CO2-fixation, reactive oxygen species, carbohydrate, phosphorus and energy metabolism have been elucidated in the book. Special emphasis has also been given to techniques on specific topics such as Electrophoresis, Molecular Biology, Histo-enzymology, Symbiotic Nitrogen Fixation and assay of plant growth hormones. Thus the present book is one stop solution for all important techniques and analytical methods for students and research workers engaged in plant sciences and agricultural research.
Plant Cells And Their Organelles
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Author : William V. Dashek
language : en
Publisher: John Wiley & Sons
Release Date : 2017-01-17
Plant Cells And Their Organelles written by William V. Dashek and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-17 with Science categories.
Plant Cells and Their Organelles provides a comprehensive overview of the structure and function of plant organelles. The text focuses on subcellular organelles while also providing relevant background on plant cells, tissues and organs. Coverage of the latest methods of light and electron microscopy and modern biochemical procedures for the isolation and identification of organelles help to provide a thorough and up-to-date companion text to the field of plant cell and subcellular biology. The book is designed as an advanced text for upper-level undergraduate and graduate students with student-friendly diagrams and clear explanations.
Cell Components
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Author : Hans-Ferdinand Linskens
language : en
Publisher: Springer
Release Date : 2012-07-01
Cell Components written by Hans-Ferdinand Linskens and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-07-01 with Science categories.
Modern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the inavailability of many new analytical methods concealed in specialized journals not normally accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete, that experiments are difficult to reproduce. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since today there is still a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and the descriptions as applied to plant material so complete in themselves that there was little need to consult other publications.
Advances In Plant Omics And Systems Biology Approaches
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Author : Flavia Vischi Winck
language : en
Publisher: Springer Nature
Release Date : 2022-02-03
Advances In Plant Omics And Systems Biology Approaches written by Flavia Vischi Winck and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-02-03 with Science categories.
In the post-genomic era, several plant species have been sequenced and massive genomic information is now available which contributed to expand the development of novel technical strategies for the study of additional levels of biological information of plant species. This book focuses on the “omics” approaches together with systems analysis of several different plant species, which have revealed very interesting variations on the cellular responses at the protein, transcript and metabolite levels in response to changes environmental conditions. The volume covers recent technological advances in the area of “omics” and synthesizes recent findings of the field of plant “omics” and systems biology together along with techniques that can be applied for such studies.