Laboratory Manual For Genetic Engineering
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Laboratory Manual For Genetic Engineering
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Author : VENNISON, S. JOHN
language : en
Publisher: PHI Learning Pvt. Ltd.
Release Date : 2009-01-01
Laboratory Manual For Genetic Engineering written by VENNISON, S. JOHN and has been published by PHI Learning Pvt. Ltd. this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-01 with Science categories.
This systematically designed laboratory manual elucidates a number of techniques which help the students carry out various experiments in the field of genetic engineering. The book explains the methods for the isolation of DNA and RNA as well as electrophoresis techniques for DNA, RNA and proteins. It discusses DNA manipulation by restriction digestion and construction of recombinant DNA by ligation. Besides, the book focuses on various methodologies for DNA transformation and molecular hybridization. While discussing all these techniques, the book puts emphasis on important techniques such as DNA isolation from Gram positive bacteria including Bacillus sp., the slot-lysis electrophoresis technique which is useful in DNA profile analysis of both Gram negative and positive bacteria, plasmid transduction in Bacillus sp., and the conjugal transfer of plasmid DNA in cyanobacteria, Bacillus and Agrobacterium tumefaciens. This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering. Besides, it will be useful for the students specializing in genetic engineering, molecular biology and molecular microbiology. KEY FEATURES : Includes about 60 different experiments. Contains several figures to reinforce the understanding of the techniques discussed. Gives useful information about preparation of stock solutions, DNA/protein conversions, restriction enzymes and their recognition sequences, and so on in Appendices.
Techniques In Genetic Engineering
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Author : Isil Aksan Kurnaz
language : en
Publisher: CRC Press
Release Date : 2015-05-08
Techniques In Genetic Engineering written by Isil Aksan Kurnaz and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-05-08 with Medical categories.
Although designed for undergraduates with an interest in molecular biology, biotechnology, and bioengineering, this book-Techniques in Genetic Engineering-IS NOT: a laboratory manual; nor is it a textbook on molecular biology or biochemistry. There is some basic information in the appendices about core concepts such as DNA, RNA, protein, genes, and
Genetic Engineering
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Author : Hala M. Abdelmigid
language : en
Publisher: CreateSpace
Release Date : 2013-11
Genetic Engineering written by Hala M. Abdelmigid and has been published by CreateSpace this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11 with Science categories.
The primary motive for compiling and publishing this manual was to provide scientists, researchers, and students from national agricultural research systems, universities, and small private companies in developing countries, as well as advanced research institutions in the developed world, with a useful guide on the protocols currently in use in genetic engineering. This manual is intended to introduce you to some of the most widely used experimental procedures in biotechnology, including DNA isolation, manipulation, and cloning. You will also gain some familiarity with some of the types of equipment frequently used in biochemistry and molecular biology.The objective of this laboratory course is to provide you with hands-on experience in some of the basic, but essential laboratory skills required in molecular biology and biotechnology. Emphasis will be placed on understanding the concepts behind designing and implementing controlled experiments. The genetic engineering laboratory, like all laboratory courses, is an exploration of procedures. This means that, in order to get full benefit from the course, you will need to read the Manual; this reading will provide background information and an outline of the procedures to be performed. If you do not do this, you will find yourself wasting large amounts of class time, and annoying both your lab partners and your instructor. To encourage your understanding of the material, you will have problem sets that cover material related to the planned experiments. The genetic engineering laboratory is conducted as a “directed” research project. This means that although the general procedures are well established, the overall goal of each experiment is the acquisition of new information. Because of the nature of scientific research, predicting the outcome of experiments that have not previously been performed is difficult. It may therefore be necessary to design new experiments based on the results of previous ones, or to repeat experiments that yielded ambiguous results. On the other hand, if you approach the course will an open and flexible mindset, you will learn how research is performed in a genetic engineering laboratory.
Plant Molecular Biology A Laboratory Manual
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Author : Melody S. Clark
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-27
Plant Molecular Biology A Laboratory Manual written by Melody S. Clark and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-27 with Science categories.
The aim of this manual is to encompass a broad range of the latest plant molecular biology techniques. However, it is acknowledged that any manual will be read (and hopefully) used by a wide range of people with different levels of experience. Hence the remit of the manual was widened to include a full range of basic molecular tech niques, so that novices do not have to consult several texts to enable the execution of each major experiment. The manual is divided into three main parts: Part I: Basic Molecular Techniques The raison d'etre behind this part is to provide a background knowledge of molecular techniques, but also to reduce duplication in later chapters (this is particularly true of the methods contained in Chap. 1). All authors provided very detailed methods and often forgot that so me of these would be covered earlier. A particular favourite was DNA extraction methods, wherc everyone managed to provide a slightly different variant! My view was that it is far less confusing for the reader to be presented with one standard protocol and accom panying troubleshooting tips, than to read a different version in each chapter. In this way the basic techniques are addressed more in depth (and my apologies to all authors for judicious use of the delete key!). RNA methodology is covered in Chapter 3. This proceeds from the fundamentals of extraction, northern blotting etc. , to cDNA libraries.
Molecular Biology Techniques
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Author : Heather B. Miller
language : en
Publisher: Academic Press
Release Date : 2011-10-18
Molecular Biology Techniques written by Heather B. Miller and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-18 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Crispr Cas
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Author : Jennifer A. Doudna
language : en
Publisher:
Release Date : 2016
Crispr Cas written by Jennifer A. Doudna and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with Genetics categories.
CRISPR/Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for de.
Laboratory Manual On Biotechnology
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Author : P. M. Swamy
language : en
Publisher: Rastogi Publications
Release Date : 2008
Laboratory Manual On Biotechnology written by P. M. Swamy and has been published by Rastogi Publications this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Biotechnology categories.
Zero To Genetic Engineering Hero
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Author : Justin Pahara
language : en
Publisher: Maker Media, Inc.
Release Date : 2021-08-19
Zero To Genetic Engineering Hero written by Justin Pahara and has been published by Maker Media, Inc. this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-08-19 with Science categories.
Zero to Genetic Engineering Hero is made to provide you with a first glimpse of the inner-workings of a cell. It further focuses on skill-building for genetic engineering and the Biology-as-a-Technology mindset (BAAT). This book is designed and written for hands-on learners who have little knowledge of biology or genetic engineering. This book focuses on the reader mastering the necessary skills of genetic engineering while learning about cells and how they function. The goal of this book is to take you from no prior biology and genetic engineering knowledge toward a basic understanding of how a cell functions, and how they are engineered, all while building the skills needed to do so.
A Practical Guide To Protein Engineering
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Author : Tuck Seng Wong
language : en
Publisher: Springer Nature
Release Date : 2020-10-29
A Practical Guide To Protein Engineering written by Tuck Seng Wong and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-29 with Science categories.
This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
Molecular Cloning
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Author : Tom Maniatis
language : en
Publisher:
Release Date : 1982
Molecular Cloning written by Tom Maniatis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982 with Science categories.