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Laboratory Protocols Cimmyt Applied Genetic Engineering Laboratory

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Laboratory Protocols Cimmyt Applied Genetic Engineering Laboratory

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Author :
language : en
Publisher: CIMMYT
Release Date : 1999

Laboratory Protocols Cimmyt Applied Genetic Engineering Laboratory written by and has been published by CIMMYT this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with categories.

Laboratory Manual For Genetic Engineering

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Author : VENNISON, S. JOHN
language : en
Publisher: PHI Learning Pvt. Ltd.
Release Date : 2009-01-01

Laboratory Manual For Genetic Engineering written by VENNISON, S. JOHN and has been published by PHI Learning Pvt. Ltd. this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-01 with Science categories.

This systematically designed laboratory manual elucidates a number of techniques which help the students carry out various experiments in the field of genetic engineering. The book explains the methods for the isolation of DNA and RNA as well as electrophoresis techniques for DNA, RNA and proteins. It discusses DNA manipulation by restriction digestion and construction of recombinant DNA by ligation. Besides, the book focuses on various methodologies for DNA transformation and molecular hybridization. While discussing all these techniques, the book puts emphasis on important techniques such as DNA isolation from Gram positive bacteria including Bacillus sp., the slot-lysis electrophoresis technique which is useful in DNA profile analysis of both Gram negative and positive bacteria, plasmid transduction in Bacillus sp., and the conjugal transfer of plasmid DNA in cyanobacteria, Bacillus and Agrobacterium tumefaciens. This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering. Besides, it will be useful for the students specializing in genetic engineering, molecular biology and molecular microbiology. KEY FEATURES : Includes about 60 different experiments. Contains several figures to reinforce the understanding of the techniques discussed. Gives useful information about preparation of stock solutions, DNA/protein conversions, restriction enzymes and their recognition sequences, and so on in Appendices.