Laser Scanning Microscopy And Quantitative Image Analysis Of Neuronal Tissue
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Laser Scanning Microscopy And Quantitative Image Analysis Of Neuronal Tissue
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Author : Lidia Bakota
language : en
Publisher: Humana
Release Date : 2016-08-27
Laser Scanning Microscopy And Quantitative Image Analysis Of Neuronal Tissue written by Lidia Bakota and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-27 with Medical categories.
Laser Scanning Microscopy and Quantitative Image Analysis of Neuronal Tissue brings together contributions from research institutions around the world covering pioneering applications in laser scanning microscopy and quantitative image analysis and providing information about the power and limitations of this quickly developing field. This detailed volume seeks to introduce key questions, to provide detailed information on how to acquire data by laser-scanning microscopy, and to examine how to use the often huge digital data set in an efficient manner to extract maximum information. Thus the book not only provides a compilation of diverse protocols but aims to bring together biological bench work, laser scanning microscopy, and mathematical, computer-assisted data analysis to grasp novel insights of form, dynamics, and interactions of microscopy-sized biological objects. Written in the popular Neuromethods series format, chapters include the kind of practical implementation advice that promises successful results. Wide-ranging and innovative, Laser Scanning Microscopy and Quantitative Image Analysis of Neuronal Tissue will stimulate the reader to make efficient use of the application of laser scanning microscopy for his or her own research question.
Improved Quantitative Analysis Of 3 D Images Of Thick Tissue Collected Using The Confocal Laser Scanning Microscope
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Author : Soraya Kayali
language : en
Publisher:
Release Date : 1996
Improved Quantitative Analysis Of 3 D Images Of Thick Tissue Collected Using The Confocal Laser Scanning Microscope written by Soraya Kayali and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with categories.
Multi Parametric Live Cell Microscopy Of 3d Tissue Models
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Author : Ruslan I. Dmitriev
language : en
Publisher: Springer
Release Date : 2017-10-26
Multi Parametric Live Cell Microscopy Of 3d Tissue Models written by Ruslan I. Dmitriev and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-26 with Science categories.
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
High Resolution Imaging In Microscopy And Ophthalmology
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Author : Josef F. Bille
language : en
Publisher: Springer
Release Date : 2019-08-13
High Resolution Imaging In Microscopy And Ophthalmology written by Josef F. Bille and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-13 with Medical categories.
This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
Visualization In Biomedical Microscopies
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Author : Andres Kriete
language : en
Publisher:
Release Date : 1992
Visualization In Biomedical Microscopies written by Andres Kriete and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Amsterdam, Netherlands categories.
Methods In Neurosciences
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Author : P. Michael Conn
language : en
Publisher: Elsevier
Release Date : 2016-07-02
Methods In Neurosciences written by P. Michael Conn and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-07-02 with Science categories.
Because of the significance of morphology and cellular communication unique to the functioning and interdependence of neural systems, many of the advances in the neurosciences rely on classic as well as new microscopy techniques. A convenient bench-top format**Methods presented for easy adaptation to new systems**Comprehensive protocols included for**Autoradiography: measurement of hormone receptors, high-resolution techniques, double labeling and combined techniques**Statistical and computational methods: video imaging, three-dimensional reconstructions, means of structural quantitation, unbiased sampling methods, measurement of analysis of neuronal connections, staining, and individual axon arbors**Tracing neuronal connections: tracer, toxin, and dye methods**Staining techniques: fluoro-gold, SITS, and silver impregnation**Freezing techniques: freeze fracture and cryoprotection**Combined and high-resolution techniques: double-label tracer techniques, incident light polarization, light and electron microscopic techniques
Three Dimensional Confocal Microscopy Volume Investigation Of Biological Specimens
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Author :
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Three Dimensional Confocal Microscopy Volume Investigation Of Biological Specimens written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems. Volume investigation Three-dimensional reconstruction Fluroescent probe design Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology Confocal data display and analysis Twenty-eight pages of color
Image Analysis
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Author : Donat P. Hader
language : en
Publisher: CRC Press
Release Date : 2000-08-23
Image Analysis written by Donat P. Hader and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-08-23 with Medical categories.
Automatic image analysis has become an important tool in many fields of biology, medicine, and other sciences. Since the first edition of Image Analysis: Methods and Applications, the development of both software and hardware technology has undergone quantum leaps. For example, specific mathematical filters have been developed for quality enhanceme
The Role Of Astroglia And Oligodendroglia In Cns Development Plasticity And Disease Novel Tools And Investigative Approaches
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Author : Enrica Boda
language : en
Publisher: Frontiers Media SA
Release Date : 2022-05-31
The Role Of Astroglia And Oligodendroglia In Cns Development Plasticity And Disease Novel Tools And Investigative Approaches written by Enrica Boda and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-05-31 with Science categories.
Confocal Laser Scanning Microscopy Of Living Cells
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Author : Alexey Moshkov
language : en
Publisher:
Release Date : 2020
Confocal Laser Scanning Microscopy Of Living Cells written by Alexey Moshkov and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with Electronic books categories.
Living cells are distinguished in their ability to keep the structural stability in the changing environment by means of energy supply. Understanding the mechanisms of energy conversion is impossible without knowledge of the physiological processes involved. This question is addressed to confocal laser scanning microscopy (CLSM) that has become a leader among the group of light microscopy method. The high resolution in CLSM images is reached without deconvolution procedures. Linear characteristics of its variable parameters are important for quantitative image analysis. These properties of CLSM are indispensable in the study of mitochondrial membrane potential (MMP) using fluorescent dyes. Lipophilic rhodamine dyes freely pass the plasma membrane and accumulate in the mitochondria according to electrical potential difference. CLSM images of TMRE (tetramethylrhodamine, ethyl ester)-stained U937 cells were analyzed by standard image processing procedures in ImageJ software. These procedures allow the creation of mask, applying it to original image. Average fluorescence intensity in selected regions was used as a measure of MMP changes during phytohemagglutinin treatment. This value was decreased by 34% after 2 h of lectin treatment. Some cells after mitogenic stimulation completely lost MMP. Deregulation of mitochondrial calcium handling and changes of cytoplasmic monovalent ion concentration are considered as mechanisms of MMP decrease during lymphocyte stimulation.