Multi Parametric Live Cell Microscopy Of 3d Tissue Models


Multi Parametric Live Cell Microscopy Of 3d Tissue Models
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Multi Parametric Live Cell Microscopy Of 3d Tissue Models


Multi Parametric Live Cell Microscopy Of 3d Tissue Models
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Author : Ruslan I. Dmitriev
language : en
Publisher: Springer
Release Date : 2017-10-26

Multi Parametric Live Cell Microscopy Of 3d Tissue Models written by Ruslan I. Dmitriev and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-26 with Science categories.


This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.



Advances In Brain Imaging Techniques


Advances In Brain Imaging Techniques
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Author : Nirmal Mazumder
language : en
Publisher: Springer Nature
Release Date : 2022-05-19

Advances In Brain Imaging Techniques written by Nirmal Mazumder and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-05-19 with Medical categories.


The book reviews the recent developments in brain imaging and their technological advancements to understand molecular mechanisms associated with neurological disorders and basic behaviors in humans and rodents at the structural, molecular, and functional levels. It discusses the usefulness of advanced optical microscopy techniques, including optical coherence tomography (OCT), miniscope, multiphoton fluorescence (2PF & 3PF), adaptive optics, harmonic generation, and Raman microscopy for understanding pathomechanism of brain disorders and pathological and physiological changes associated with neurodegenerative diseases. Also, the book presents conventional imaging modalities, including Magnetic Resonance Imaging (MRI), for delineating underlying mechanisms and precise early diagnosis of neurological disorders. This book is a useful resource for neuroscientists and researchers working in biomedical engineering and optics.



Intestinal Differentiated Cells


Intestinal Differentiated Cells
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Author : Paloma Ordóñez-Morán
language : en
Publisher: Springer Nature
Release Date : 2023-06-13

Intestinal Differentiated Cells written by Paloma Ordóñez-Morán and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-06-13 with Science categories.


This volume details the most up-to-date methods of intestinal differentiation program. Chapters guide readers through techniques to characterize intestinal differentiated cell, RNAseq, single-cell RNAseq transcriptional profiling methods, isolation of intestinal crypts, and methods for studying intestinal differentiated cancer cells. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Intestinal Differentiated Cells: Methods and Protocols aims to be comprehensive guide for researchers.



Quenched Phosphorescence Detection Of Molecular Oxygen


Quenched Phosphorescence Detection Of Molecular Oxygen
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Author : Dmitri B Papkovsky
language : en
Publisher: Royal Society of Chemistry
Release Date : 2018-05-25

Quenched Phosphorescence Detection Of Molecular Oxygen written by Dmitri B Papkovsky and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-25 with Science categories.


Significant progress has been made in recent years in quenched-phosphorescence oxygen sensing, particularly in the materials and applications of this detection technology that are open to commercialization, like uses in brain imaging and food packaging. Prompted by this, the editors have delivered a dedicated book that brings together these developments, provides a comprehensive overview of the different detection methodologies, and representative examples and applications. This book is intended to attract new researchers from various disciplines such as chemistry, physics, biology and medicine, stimulate further progress in the field and assist in developing new applications. Providing a concise summary at the cutting edge, this practical guide for current experts and new potential users will increase awareness of this versatile sensing technology.



Engineering 3d Tissue Test Systems


Engineering 3d Tissue Test Systems
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Author : Karen J.L. Burg
language : en
Publisher: CRC Press
Release Date : 2017-07-28

Engineering 3d Tissue Test Systems written by Karen J.L. Burg and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-28 with Medical categories.


Engineering 3D Tissue Test Systems provides an introduction to, and unique coverage of, a rapidly evolving area in biomaterials engineering. It reveals the current and future research responses, the current and future diagnostic applications, and provides a comprehensive overview to foster innovation. It offers insight into the importance of 3D systems and their use as benchtop models, spanning applications from basic scientific research to clinical diagnostics. Methods and limitations of building 3D tissue structures are evaluated, with attention given to the cellular, polymeric, and fabrication instrumentation components. The book covers the important aspects of polymeric tissue test systems, highlighting the needs and constraints of the industry, and includes a chapter on regulatory and pricing issues.



Modern Tools For Time Resolved Luminescence Biosensing And Imaging


Modern Tools For Time Resolved Luminescence Biosensing And Imaging
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Author :
language : en
Publisher: Frontiers Media SA
Release Date : 2021-09-01

Modern Tools For Time Resolved Luminescence Biosensing And Imaging written by and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-09-01 with Science categories.




Advanced Time Correlated Single Photon Counting Applications


Advanced Time Correlated Single Photon Counting Applications
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Author : Wolfgang Becker
language : en
Publisher: Springer
Release Date : 2015-04-13

Advanced Time Correlated Single Photon Counting Applications written by Wolfgang Becker and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-04-13 with Science categories.


This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.



Progress In Photon Science


Progress In Photon Science
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Author : Kaoru Yamanouchi
language : en
Publisher: Springer
Release Date : 2019-01-23

Progress In Photon Science written by Kaoru Yamanouchi and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-01-23 with Science categories.


This second volume of “Progress in Photon Science – Recent Advances” presents the latest achievements made by world-leading researchers in Russia and Japan. Thanks to recent advances in light source technologies; detection techniques for photons, electrons, and charged particles; and imaging technologies, the frontiers of photon science are now being expanding rapidly. Readers will be introduced to the latest research efforts in this rapidly growing research field through topics covering bioimaging and biological photochemistry, atomic and molecular phenomena in laser fields, laser–plasma interaction, advanced spectroscopy, electron scattering in laser fields, photochemistry on novel materials, solid-state spectroscopy, photoexcitation dynamics of nanostructures and clusters, and light propagation.



3d Bioprinting


3d Bioprinting
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Author : Dong-Woo Cho
language : en
Publisher: Springer Nature
Release Date : 2019-12-04

3d Bioprinting written by Dong-Woo Cho and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-12-04 with Medical categories.


This text advances fundamental knowledge in modeling in vitro tissues/organs as an alternative to 2D cell culture and animal testing. Prior to engineering in vitro tissues/organs,the descriptions of prerequisites (from pre-processing to post-processing) in modeling in vitro tissues/organs are discussed. The most prevalent technologies that have been widely used for establishing the in vitro tissue/organ models are also described, including transwell, cell spheroids/sheets, organoids, and microfluidic-based chips. In particular, the authors focus on 3D bioprinting in vitro tissue/organ models using tissue-specific bioinks. Several representative bioprinting methods and conventional bioinks are introduced. As a bioink source, decellularized extracellular matrix (dECM) are importantly covered, including decellularization methods, evaluation methods for demonstrating successful decellularization, and material safety. Taken together, the authors delineate various application examples of 3D bioprinted in vitro tissue/organ models especially using dECM bioinks.



Biofabrication And 3d Tissue Modeling


Biofabrication And 3d Tissue Modeling
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Author : Dong-Woo Cho
language : en
Publisher: Royal Society of Chemistry
Release Date : 2019-01-02

Biofabrication And 3d Tissue Modeling written by Dong-Woo Cho and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-01-02 with Technology & Engineering categories.


3D tissue modelling is an emerging field used for the investigation of disease mechanisms and drug development. The two key drivers of this upsurge in research lie in its potential to offer a way to reduce animal testing with respect to biotoxicity analysis, preferably on physiology recapitulated human tissues and, additionally, it provides an alternative approach to regenerative medicine. Integrating physics, chemistry, materials science, and stem cell and biomedical engineering, this book provides a complete foundation to this exciting, and interdisciplinary field. Beginning with the basic principles of 3D tissue modelling, the reader will find expert reviews on key fabrication technologies and processes, including microfluidics, microfabrication technology such as 3D bioprinting, and programming approaches to emulating human tissue complexity. The next stage introduces the reader to a range of materials used for 3D tissue modelling, from synthetic to natural materials, as well as the emerging field of tissue derived decellularized extracellular matrix (dECM). A whole host of critical applications are covered, with several chapters dedicated to hard and soft tissues, as well as focused reviews on the respiratory and central nervous system. Finally, the development of in vitro tissue models to screen drugs and study progression and etiologies of diseases, with particular attention paid to cancer, can be found.