Lethal Genes

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Lethal Genes

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Author : Linda Grant
language : en
Publisher: Scribner Book Company
Release Date : 1996

Lethal Genes written by Linda Grant and has been published by Scribner Book Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Fiction categories.

In San Francisco, PI Catherine Sayler investigates sabotage in a university's agricultural laboratory. Someone is killing researchers working on better ways to grow corn. Suspects include a competing biotechnical firm. By the author of A Woman's Place.

Complementary Lethal Genes In Wheat

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Author : Ralph Merrill Caldwell
language : en
Publisher:
Release Date : 1943

Complementary Lethal Genes In Wheat written by Ralph Merrill Caldwell and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1943 with categories.

Mapping Hybrid Lethal Genes On The X Chromosome Of C Briggsae

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Author : Blaine E. Bittorf
language : en
Publisher:
Release Date : 2018

Mapping Hybrid Lethal Genes On The X Chromosome Of C Briggsae written by Blaine E. Bittorf and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Bacterial artificial chromosomes categories.

In the cross of C. nigoni males to C. briggsae hermaphrodites, all F1 males arrest during embryogenesis. However in the reciprocal cross there are some viable F1 male progeny. This unidirectional male-specific lethality in the F1 hybrids has been attributed to a hybrid lethal gene in a 500 Kb region of the X chromosome of C. briggsae. Cbr-him-8 is a recessive maternal suppressor of the male-specific lethal phenotype, due to the requirement of the him-8 protein for proper X chromosome pairing. Without proper pairing of any one of the chromosomes in the Caenorhabditis genome, genes present on the unpaired chromosome will be silenced due to a process known as meiotic silencing of unpaired chromosomes (MSUC). It has been proposed that MSUC-based silencing of the X-linked hybrid lethal gene is the mechanism by which the male-specific lethality is suppressed. Based on this model, a co-suppression assay was used to identify the hybrid lethal gene. Transgenic strains of C. briggsae were constructed via microinjection of bacterial artificial chromosomes (BACs) of small portions of the X chromosome in which the hybrid lethal gene resides. The BACs were mixed with pCFJ909, a plasmid containing a functional cbr-unc-119 gene, this mixture was then microinjected directly into the gonad of cbr-unc-119 mutant hermaphrodites. A proportion of the resulting progeny incorporated the injected DNA into their nucleus and formed heritable extra-chromosomal arrays. These offspring were then selected based on the rescue of the unc-119 phenotype. Transgenic hermaphrodites were then mated to C. nigoni males and scored for viable F1 male progeny. Two BAC rescued the male specific hybrid lethal phenotype. Multiple other BACs failed to rescue the lethality phenotype. Focusing on a single BAC clone, using gene groupings and pCFJ909 the number of possible genes have been narrowed to two candidate hybrid lethal genes within the BAC 08G05. As well as 5 candidate hybrid lethal genes in the non-adjacent BAC 17D03.

A Survey Of The Types Of Lethal Genes And Lethal Effects Found In Plants

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Author : Mary Elizabeth Sanders
language : en
Publisher:
Release Date : 1940

A Survey Of The Types Of Lethal Genes And Lethal Effects Found In Plants written by Mary Elizabeth Sanders and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1940 with Heredity categories.

The Action Of Lethal Genes In Mormoniella Vitripennis

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Author : Rosalie Elizabeth Dennis
language : en
Publisher:
Release Date : 1961

The Action Of Lethal Genes In Mormoniella Vitripennis written by Rosalie Elizabeth Dennis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1961 with categories.

Identification Of A Hybrid Lethal Gene On The X Chromosome Of Caenorhabditis Briggsae

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Author : John Kelly Dougherty
language : en
Publisher:
Release Date : 2019

Identification Of A Hybrid Lethal Gene On The X Chromosome Of Caenorhabditis Briggsae written by John Kelly Dougherty and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with Bacterial artificial chromosomes categories.

Two closely related Caenorhabditis species, C. briggsae and C. nigoni are cross fertile and produce viable adult progeny. From C. nigoni mothers, F1 adult females are viable and fertile, F1 males are viable but sterile. In crosses that utilize C. nigoni males and C. briggsae hermaphrodites produce viable adult F1 females but F1 males arrest during embryogenesis. A mutation in the Cbr-him-8 gene is a recessive maternal-effect suppressor of male-specific lethality. Hybrid crosses with cbr-him-8 mutant mothers produce viable adult male progeny. The HIM-8 protein in C. elegans is required for the pairing of X-chromosomes during meiosis. This function is likely conserved in C. briggsae. Unpaired chromosomes are transcriptionally silenced in a wide variety of taxa. Based on this information it's been proposed that the meiotic silencing of unpaired chromosomes (MSUC) is suppressing an X-linked hybrid lethal gene responsible for male specific lethality. Multiple co-suppression assays identified two genes as candidate hybrid lethal genes, CBG30927 and CBG00239. These genes were knocked out with RNAi and CRISPR to evaluate if either of these genes were a hybrid lethal gene. sgRNA/Cas9 complexes and dsRNA of the candidate hybrid lethal genes was injected into C. briggsae hermaphrodites. Injections using CRISPR were able to disrupt expression of control targets but not the candidate hybrid lethal genes. Both RNAi and CRISPR injected nematodes were mated with C. nigoni males and the resulting progeny were scored for viable F1 males. From injections of dsRNA containing copies of CBG30927 or Cbr-hig-1, male progeny were derived. Cbr-hig-1 has a syntenic ortholog in C. nigoni that is not present in any other Caenorhabditis species. Several regions were identified in the C. briggsae and C. nigoni transcripts including an exon 5 extension that is responsible for a change in the predicted structure of the proteins that could be responsible for the dysgenic interactions.

Characterization Of The Recessive Lethal Gene Sup61 From Saccharomyces Cerevisiae

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Author : Maria Tina Johnson Etcheverry
language : en
Publisher:
Release Date : 1980

Characterization Of The Recessive Lethal Gene Sup61 From Saccharomyces Cerevisiae written by Maria Tina Johnson Etcheverry and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1980 with Lethal mutation categories.

Lethal Alleles

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Author : Eileen M. Gross
language : en
Publisher:
Release Date : 2001

Lethal Alleles written by Eileen M. Gross and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with Genetic engineering in art categories.

Further Studies On The Action Of Lethal Genes In Mormoniella Vitripennis

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Author : Anita Y. Nance
language : en
Publisher:
Release Date : 1962

Further Studies On The Action Of Lethal Genes In Mormoniella Vitripennis written by Anita Y. Nance and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1962 with Dissertations, Academic categories.

Synthetic Temperature Inducible Lethal Genetic Circuits In Escherichia Coli

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Author : Stephanie Pearce
language : en
Publisher:
Release Date : 2016

Synthetic Temperature Inducible Lethal Genetic Circuits In Escherichia Coli written by Stephanie Pearce and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.

Temperature-sensitivity (TS) is often used as a way to attenuate microorganisms to convert them into live vaccines. Studies indicate that live vaccines are often necessary for the complete clearance of certain pathogenic organisms. In this work we explore the use of TS genetic circuits that express lethal genes for their potential utility as a widely applicable approach to TS attenuation. Here, we use restriction endonucleases as the lethal gene products. We tested different combinations of TS repressors and cognate promoters controlling the expression of genes encoding restriction endonucleases inserted at four different non-essential sites in the Escherichia coli chromosome. We found that the presence of the restriction endonuclease genes did not affect the viability of the host strains at the permissive temperature, but that expression of the genes at elevated temperatures killed the strains to varying extents. The location of the genetic circuit cassette in the chromosome was critical, and insertion at the ycgH site led to minimal cell death. Induction of the TS circuit in a growing culture led to a pre-mature leveling off of the optical density, and a shift in the number of cells that could exclude a dye that indicated cell viability. Incubation of cells initially grown at low temperature and then suspended in phosphate buffered saline at high temperature, led to about 100-fold loss of cell viability per day compared to minimal loss of viability for the parental strain. The Dual strain containing two different genetic circuits was found to have reduced escape frequency compared to single circuit strains. However, strains carrying either one or two TS lethal circuits could generate mutants that survived high temperature. These mutants included start codon deletions as well as upstream deletions of the TetRD1 encoding gene as well as complete deletions of the lethal gene circuits.