Lumi Imagertm F1
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Lumi Imagertm F1
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Author : Onno Bakker
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-17
Lumi Imagertm F1 written by Onno Bakker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-17 with Science categories.
The instrument Lumi-Imager was developed for the detection and accurate quantification of chemiluminescent signals on blots and in microtiter plates. It contains a cooled CCD camera, a specially developed lens system and software. In addition to luminescence, Lumi-Imager F1 can also detect and quantify UV-excited fluorescent signals in gels. Here we describe the components and features of this instrument as well as some of its applications.
Lumi Imager F1
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Author : Onno Bakker
language : en
Publisher:
Release Date : 2014-01-15
Lumi Imager F1 written by Onno Bakker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-15 with categories.
Development Of Methods For Directed And Structured Immobilization Of Biomolecules
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Author : Björn Waterkotte
language : en
Publisher: Cuvillier Verlag
Release Date : 2013-11-07
Development Of Methods For Directed And Structured Immobilization Of Biomolecules written by Björn Waterkotte and has been published by Cuvillier Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-07 with Science categories.
Surface immobilized molecules play a crucial role in applied as well as in basic research. They can be found in DNA or protein-array as well as in cell-migration or biochemical interaction experiments. Especially for proteins the immobilization can critically affect molecule accessibility and activity. In this work two essential aspects of protein immobilization were investigated: the identification of suitable surface functionalization by two array approaches and the generation of laterally structured gray scale protein patterns by projection lithography. In proof-of-concept experiments a new strategy to create an array of different, neighboring functionalizations on the same surface was successfully tested. In "mechanical protection" inert plungers were applied to protect distinct surface areas from the surface functionalization reagents and allowed a selective modification of individual regions in serial reactions. In a particle based alternative approach a high spot density surface array was fabricated using functionalized, polyvinyl acetate, melamine and silicon oxide-based particles. The in situ encoded bead-based array (IEBA) allowed the study of immobilization conditions and the effect of protein adhesion. For the reliable and fast analysis of array-images with several thousand individual particles a custom open-source evaluation software was developed. To create protein patterns a custom-built projection lithography device was utilized to generate high definition protein patterns. Patterning conditions were optimized with respect to deposition speed and signal to noise ratio while retaining a biocompatible process flow. The established technology provides protein patterns with great control over the deposited surface density, patterns were transferred within seconds to minutes to functionalized glass slides. To obtain three-dimensional, surface-functionalized surfaces this approach was transferred to thin polymer films. The substrates were demonstrated to be biocompatible with mouse fibroblasts. These surfaces allow a novel level of control addressing both the chemical surrounding and the shape of a cell microenvironment.
Liposomes Part B
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Author : Nejat Duzgunes
language : en
Publisher: Elsevier
Release Date : 2003-11-19
Liposomes Part B written by Nejat Duzgunes and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-11-19 with Science categories.
Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes in Biochemistry Liposomes in Molecular Cell Biology Liposomes in Molecular Virology
Research Advances In The Study Of Campylobacter Helicobacter Related Organisms
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Author : D Scott Merrell
language : en
Publisher: Frontiers E-books
Release Date :
Research Advances In The Study Of Campylobacter Helicobacter Related Organisms written by D Scott Merrell and has been published by Frontiers E-books this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Campylobacter spp and Helicobacter spp are gastrointestinal pathogens that remain a major cause of acute gastroenteritis and gastric disease, respectively. The 16th International Workshop on Campylobacter, Helicobacter and Related Organisms (CHRO) will be held in Vancouver, British Columbia Canada from August 28-September 1, 2011 and will highlight recent advances in our understanding of the epidemiology, survival mechanisms, host response and pathogenesis of these important species. This Research Topic issue will highlight each of these topics and will attempt to shed insight into our growing understanding of the process of host-pathogen interactions as it relates to Campylobacter and Helicobacter.
Perspectives For The Next Generation Of Virus Research Spearheading The Use Of Innovative Technologies And Methodologies
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Author : Takatoki Yamamoto
language : en
Publisher: Frontiers Media SA
Release Date : 2017-07-06
Perspectives For The Next Generation Of Virus Research Spearheading The Use Of Innovative Technologies And Methodologies written by Takatoki Yamamoto and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-06 with categories.
Infectious diseases are associated with approximately 20% of global mortality, with viral diseases causing about one third of these deaths. Besides newly emerging and re-emerging viral infections will continue to pose a threat to human survival globally. In this case scientific advances have greatly been increased to defend against those pathogens. For example, rapid genomic sequencing, proteomics, epigenomics, nanotechnology, and other advanced tools are being applied to detect viruses at the point of care and to track their spread within human populations as well as to understand virus-host interaction and virus induced pathogenesis. From rapid identification of new viruses to prevention with vaccination and treatment with effective therapeutics, biomedical research has continuously provided tools to meet the constant threat of emerging viral pathogens. Despite these advances, each new disease brings unique challenges to scientists every year. So we must stay at the cutting edge of scientific discovery, working energetically to develop new tools to combat the ever-changing threats they pose. Our research topic highlights such advanced and new technology based virus research which definitely bolsters the researcher's ability to tackle emerging, re-emerging and stable viral pathogens. We are credulous that the papers including in the e-books will be beneficial to the experts in the field to understand the molecular, immunological, ecological and clinical aspects of the next generation researches for the prevention and control of infectious diseases caused by viruses.
Autophagy In Mammalian Systems Part B
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Author : Daniel Klionsky
language : en
Publisher: Academic Press
Release Date : 2009-03-05
Autophagy In Mammalian Systems Part B written by Daniel Klionsky and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-03-05 with Science categories.
This is the companion volume to Daniel Klionsky’s Autophagy: Lower Eukaryotes, which features the basic methods in autophagy covering yeasts and alternative fungi (aspergillus, podospora, magnaporthe). Klionsky is one of the leading authorities in the field. He is the editor-in-chief of Autophagy. The November 2007 issue of Nature Reviews highlighted his article, “Autophagy: From phenomenology to molecular understanding in less than a decade. He is currently editing guidelines for the field, with 230 contributing authors, that will publish in Autophagy. Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal “house cleaning in the cell is called autophagy – literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 (autophagy-related gene 1) in yeast. (To date, 30 additional yeast genes have been identified.) These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth.
Surveying Antimicrobial Resistance The New Complexity Of The Problem
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Author : Gilberto Igrejas
language : en
Publisher: Frontiers Media SA
Release Date : 2020-07-10
Surveying Antimicrobial Resistance The New Complexity Of The Problem written by Gilberto Igrejas and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-10 with categories.
In January of 2015, under the 1st International Caparica Conference in Antibiotic Resistance, a Research Topic entitled: “Surveying Antimicrobial Resistance: Approaches, Issues, and Challenges to overcome”, was published (http://journal.frontiersin.org/researchtopic/3763/surveying-antimicrobial-resistanceapproaches- issues-and-challenges-to-overcome). The problem of antimicrobial resistance (AMR), caused by excessive and inappropriate use of antibiotics, is a public health issue that concerns us all. The introduction of penicillin in the 1940s, the start of the antibiotics era, has been recognized as one of the greatest advances in therapeutic medicine. However, according to the World Health Organization (WHO), AMR infections are now an increasing worldwide public health threat and a post-antibiotic era is imminent, where common infections and minor injuries could be fatal. AMR is a typical ‘One Health’ problem, in which livestock animals and the environment constitute AMR reservoirs and transmission routes to and from the human population. Without effective antimicrobials to counter and prevent infections, other major achievements in modern medicine, such as organ transplantation, cancer chemotherapy and major surgery, risk being compromised. AMR infections in animals have negative outcomes on animal health, welfare, biosecurity and production. In 2006, the ban of growth promoting antibiotics highlighted antibiotic use in animal production as a risk factor in the development of antibiotic resistant bacteria. Bacteria can be transferred to humans via several routes; consumption of animal products, exposure through contact with animals, and the contamination of ground and surface waters by animal waste products. Therefore, it is of utmost importance that antimicrobial use in animals is reduced to a minimum, without compromising animal health and welfare. Mechanisms of bacterial antibiotic resistance are classified according to the types of antibiotic molecules or their targets in the cell. Environmental antibiotic-resistance genes are spread then acquired by clinically relevant microorganisms. Many resistance genes are conveyed into pathogen genomes via mobile genetic elements such as plasmids, transposons or integrons, increasing the propagation of potential resistant pathogens. Substantial progress has already been made in elucidating the basic regulatory networks that endow bacteria with their extraordinary capacity to adapt to a diversity of lifestyles and external stress factors. So how will we face bacteria in the future?
The Protein Protocols Handbook
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Author : John M. Walker
language : en
Publisher: Springer
Release Date : 2007-10-02
The Protein Protocols Handbook written by John M. Walker and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-02 with Science categories.
The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Science
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Author : John Michels (Journalist)
language : en
Publisher:
Release Date : 2001-08
Science written by John Michels (Journalist) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-08 with Science categories.