Magnetic Cell Separation
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Magnetic Cell Separation
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Author :
language : en
Publisher: Elsevier
Release Date : 2011-08-31
Magnetic Cell Separation written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-31 with Science categories.
Cell separation is at the core of current methods in experimental biology and medicine. Its importance is illustrated by the large number of physical and biochemical principles that have been evaluated for application to cell separation. The development of cell separation methods is driven by the needs of biological and medical research, and the ever-increasing demands for sensitivity, selectivity, yield, timeliness and economy of the process. The interdisciplinary nature of research in this area and the volume of information available in research publications and conferences necessitates a basic description of the fundamental processes involved in magnetic cell separation that may help the user in navigating this wealth of information available online and in scientific publications. This book will appeal to researchers in many areas utilizing this technique, including those working in cell biology, clinical research, inorganic chemistry, biochemistry, chemical engineering, materials science, physics and electrical engineering. Provides examples of how to calculate the volume magnetic susceptibility, a fundamental quantity for calculating the magnetic force acting on a cell, from various types of magnetic susceptibilities available in literature Introduces the elements of magnetostatics as they apply to cell magnetization and the magnetization of magnetic micro- and nano- particles used for cell separation Describes the parameters used to determine cell magnetophoresis
Magnetic Cell Separation Method
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Author :
language : en
Publisher:
Release Date :
Magnetic Cell Separation Method written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Mems Micro Electro Mechanical Systems Based Microfluidic Platforms For Magnetic Cell Separation
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Author : Pulak Nath
language : en
Publisher:
Release Date : 2008
Mems Micro Electro Mechanical Systems Based Microfluidic Platforms For Magnetic Cell Separation written by Pulak Nath and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Magnetic separation categories.
Microfluidic platforms for magnetic cell separation were developed and investigated for isolation of magnetic particles and magnetically tagged cells from a fluidic sample. Two types of magnetic separation platforms were considered: an Isodynamic Open Gradient Magnetic Sorter (OGMS) and a multistage bio-ferrograph. Miniaturized magnets were designed using magnetostatic simulation software, microfluidic channels were fabricated using microfabrication technology and magnetic separation was investigated using video microscopy and digital image processing. The isodynamic OGMS consisted of an external magnetic circuit and a microfabricated channel (biochip) with embedded magnetic elements. The biochip is placed inside the magnetic field of the external circuit to obtain nearly constant energy density gradient in the portion of the channel used for separation. The microfabrication process involved improving adhesion of thick SU-8 to Pyrex, forming enclosed channels using a low temperature SU-8 adhesive bonding, and fabricating patterned plating molds on both sides of the bonded wafers. Adhesion of SU-8 to Pyrex was improved by using a highly crosslinked thin SU-8 adhesion layer, and enclosed microchannels were fabricated using selectively exposed SU-8 bond formation layers. Electroplating molds were fabricated using KMPR photoresists and were integrated on both sides of the bonded wafers. The multistage bio-ferrograph consisted of a microfabricated enclosed channel placed on the surface of a multi-unit magnet (4 trapezoidal magnets placed in series) assembly such that magnetic cells from a flowing stream would be deposited on designated locations. The OGMS was able to deflect magnetic particles by 500-1000 microns and the capture efficiencies of magnetic particles and cells with the multistage bio-ferrograph were 80-85 percent and 99.5 percent, respectively.
Cell Separation Methods And Applications
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Author : Diether Recktenwald
language : en
Publisher: CRC Press
Release Date : 1997-11-04
Cell Separation Methods And Applications written by Diether Recktenwald and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-11-04 with Medical categories.
"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "
Cell Separation
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Author : Thomas G. Pretlow
language : en
Publisher: Academic Press
Release Date : 2014-05-10
Cell Separation written by Thomas G. Pretlow and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-10 with Science categories.
Cell Separation: Methods and Selected Applications is a compendium of articles on the design and/or application of methods for the separation of cells. This volume presents contributions on relatively finite subjects on cell separation. It covers topics on cell separation such as methods for obtaining cells in suspension from animal tissues; some of the kinds of data that are helpful in the description of cell purifications; and separation of host cells infiltrating tumors and allografts by velocity sedimentation at unit gravity. The separation of different kinds of nucleated cells from blood by centrifugal elutriation; a new approach to the separation of cells at unit gravity; and the isolation and culture of homogeneous populations of glomerular cell types are elucidated as well. Experimental oncologists, hematologists, immunologists, cell biologists, endocrinologists, and others who are not already expert in the use of methods for cell separation will find the book highly useful.
Microtechnology For Cell Manipulation And Sorting
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Author : Wonhee Lee
language : en
Publisher: Springer
Release Date : 2016-10-05
Microtechnology For Cell Manipulation And Sorting written by Wonhee Lee and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-05 with Technology & Engineering categories.
This book delves into the recent developments in the microscale and microfluidic technologies that allow manipulation at the single and cell aggregate level. Expert authors review the dominant mechanisms that manipulate and sort biological structures, making this a state-of-the-art overview of conventional cell sorting techniques, the principles of microfluidics, and of microfluidic devices. All chapters highlight the benefits and drawbacks of each technique they discuss, which include magnetic, electrical, optical, acoustic, gravity/sedimentation, inertial, deformability, and aqueous two-phase systems as the dominant mechanisms utilized by microfluidic devices to handle biological samples. Each chapter explains the physics of the mechanism at work, and reviews common geometries and devices to help readers decide the type of style of device required for various applications. This book is appropriate for graduate-level biomedical engineering and analytical chemistry students, as well as engineers and scientists working in the biotechnology industry.
Magnetic Materials And Technologies For Medical Applications
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Author : Alexander Tishin
language : en
Publisher: Woodhead Publishing
Release Date : 2021-11-18
Magnetic Materials And Technologies For Medical Applications written by Alexander Tishin and has been published by Woodhead Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-11-18 with Technology & Engineering categories.
The study of electromagnetic fields in the treatment of various diseases is not a new one; however, we are still learning how magnetic fields impact the human body and its organs. Many novel magnetic materials and technologies could potentially transform medicine. Magnetic Materials and Technologies for Medical Applications explores these current and emerging technologies. Beginning with foundational knowledge on the basics of magnetism, this book then details the approaches and methods used in the creation of novel magnetic materials and devices. This book also discusses current technologies and applications, as well as the commercial aspects of introducing new technologies to the field. This book serves as an excellent introduction for early career researchers or a reference to more experienced researchers who wish to stay abreast of current trends and developing technologies in the field. This book could also be used by clinicians working in medicine and companies interested in establishing new medical technologies. Each chapter provides novel tasks for future scientific and technology research studies. Outlines the basics of magnetism for enhanced understanding of its applications in medicine Covers novel magnetic devices as well as technologies still under development, including magnetic brain stimulation, biosensors, and nanoparticles for drug delivery Explores commercial opportunities and obstacles to market entry for new magnetic materials and technologies for the medical field
Bioprocessing For Cell Based Therapies
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Author : Che J. Connon
language : en
Publisher: John Wiley & Sons
Release Date : 2017-02-06
Bioprocessing For Cell Based Therapies written by Che J. Connon and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-02-06 with Science categories.
With contributions from leading, international academics and industrial practitioners, Bioprocessing for Cell-Based Therapies explores the very latest techniques and guidelines in bioprocess production to meet safety, regulatory and ethical requirements, for the production of therapeutic cells, including stem cells. An authoritative, cutting-edge handbook on bioprocessing for the production of therapeutic cells with extensive illustrations in full colour throughout An authoritative, cutting-edge handbook on bioprocessing for the production of therapeutic cells with extensive illustrations in full colour throughout In depth discussion of the application of cell therapy including methods used in the delivery of cells to the patient Includes contributions from experts in both academia and industry, combining a practical approach with cutting edge research The only handbook currently available to provide a state of the art guide to Bioprocessing covering the complete range of cell-based therapies, from experts in academia and industry
Methods Of Cell Separation
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Author : P.T. Sharpe
language : en
Publisher: Elsevier
Release Date : 1988-05-01
Methods Of Cell Separation written by P.T. Sharpe and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988-05-01 with Science categories.
Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; non-specific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments. A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method. Methods of Cell Separation is distinguished by three powerful assets: descriptions of the majority of cell separation methods currently being used; details of the experimental procedures involved in each method; and comparisons of the different methods for separating cell populations with particular reference to blood lymphocytes. An excellent addition to a distinguished series, and extremely useful as a laboratory manual.
Stem Cell Bioprocessing
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Author : Tiago G Fernandes
language : en
Publisher: Elsevier
Release Date : 2013-11-15
Stem Cell Bioprocessing written by Tiago G Fernandes and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-15 with Science categories.
Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described, including their applications for stem cell expansion, and stem cell separation techniques such as isolation and purification are discussed. Basic definitions are provided concerning the different types of stem cells, from adult stem cells to the more recent induced pluripotent stem cells. The main characteristics of these different stem cell types are described, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xeno-free culture conditions, as well as culture parameters that influence stem cell culture. Approaches for both stem cell culture and separation in micro-scale conditions are presented, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules. A further section is dedicated to application of stem cells for regenerative medicine. Maintains a unique focus on both the basic stem cell biology concepts, and their translation to large-scale bioprocessing approaches Envisages the use of stem cells in regenerative medicine and drug screening applications Discusses the application of microscale techniques as a tool to perform basic stem cell biology studies