Maintaining And Troubleshooting Hplc Systems
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Maintaining And Troubleshooting Hplc Systems
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Author : Dennis J. Runser
language : en
Publisher:
Release Date : 1981-05-06
Maintaining And Troubleshooting Hplc Systems written by Dennis J. Runser and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981-05-06 with Reference categories.
Provides users of HPLC equipment with a comprehensive text for troubleshooting and maintaining HPLC systems. Describes how the chromatographer can maintain the HPLC system in operating condition, what to look for and do to prevent and solve HPLC problems, and what can and should be done before calling a service representative. Organized into chapters which basically represent the typical components of the HPLC system, with each chapter describing a basic element of the HPLC system in terms of maintenance and solving system problems. Arranged as a guide and working manual to help the chromatographer reduce instrument downtime, allowing for more efficiency and cost effectiveness in the HPLC laboratory.
Hplc And Uhplc For Practicing Scientists
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Author : Michael W. Dong
language : en
Publisher: John Wiley & Sons
Release Date : 2019-07-10
Hplc And Uhplc For Practicing Scientists written by Michael W. Dong and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-10 with Science categories.
A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter. Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects Includes end-of-chapter quizzes as assessment and learning aids Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher.
Modern Hplc For Practicing Scientists
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Author : Michael W. Dong
language : en
Publisher: John Wiley & Sons
Release Date : 2016-04-06
Modern Hplc For Practicing Scientists written by Michael W. Dong and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-06 with Science categories.
A comprehesive yet concise guide to Modern HPLC Written for practitioners by a practitioner, Modern HPLC for Practicing Scientists is a concise text which presents the most important High-Performance Liquid Chromatography (HPLC) fundamentals, applications, and developments. It describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. Moreover, the book serves well as an updated reference guide for busy laboratory analysts and researchers. Topics covered include: HPLC operation Method development Maintenance and troubleshooting Modern trends in HPLC such as quick-turnaround and "greener" methods Regulatory aspects While broad in scope, this book focuses particularly on reversed-phase HPLC, the most common separation mode, and on applications for the pharmaceutical industry, the largest user segment. Accessible to both novice and intermedate HPLC users, information is delivered in a straightforward manner illustrated with an abundance of diagrams, chromatograms, tables, and case studies, and supported with selected key references and Web resources. With intuitive explanations and clear figures, Modern HPLC for Practicing Scientists is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology.
Handbook Of Pharmaceutical Analysis By Hplc
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Author : Satinder Ahuja
language : en
Publisher: Elsevier
Release Date : 2005-02-09
Handbook Of Pharmaceutical Analysis By Hplc written by Satinder Ahuja and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-02-09 with Medical categories.
High pressure liquid chromatography–frequently called high performance liquid chromatography (HPLC or, LC) is the premier analytical technique in pharmaceutical analysis and is predominantly used in the pharmaceutical industry. Written by selected experts in their respective fields, the Handbook of Pharmaceutical Analysis by HPLC Volume 6, provides a complete yet concise reference guide for utilizing the versatility of HPLC in drug development and quality control. Highlighting novel approaches in HPLC and the latest developments in hyphenated techniques, the book captures the essence of major pharmaceutical applications (assays, stability testing, impurity testing, dissolution testing, cleaning validation, high-throughput screening). A complete reference guide to HPLC Describes best practices in HPLC and offers 'tricks of the trade' in HPLC operation and method development Reviews key HPLC pharmaceutical applications and highlights currents trends in HPLC ancillary techniques, sample preparations, and data handling
Pesticide Analytical Manual
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Author : United States. Food and Drug Administration
language : en
Publisher:
Release Date : 1994
Pesticide Analytical Manual written by United States. Food and Drug Administration and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Food categories.
Troubleshooting Hplc Systems
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Author : Paul C. Sadek
language : en
Publisher: Wiley-Interscience
Release Date : 2000
Troubleshooting Hplc Systems written by Paul C. Sadek and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Science categories.
A complete, up-to-date guide to the use, maintenance, and troubleshooting of HPLC systems The last twenty-five years have seen a dramatic rise in the use of High Performance Liquid Chromatography (HPLC) in laboratories worldwide. Troubleshooting HPLC Systems provides analysts as well as laboratory technicians and managers with a readily accessible and immensely useful guide to the new generation of HPLC equipment and techniques. With an emphasis on effective troubleshooting of HPLC systems, this lab companion covers system configuration and functions, problem-solving procedures, maintenance, and HPLC basics. It then walks chromatographers investigating the source of a malfunction through each system component-from solvents and reservoirs to sample preparation to columns and detectors. Special features of Troubleshooting HPLC Systems include: * A detailed review of HPLC instrumentation and accessories * The role of operating parameters as indicators of system performance * Step-by-step troubleshooting protocols for each system component * How to set up a preventive maintenance program for HPLC systems * An overview of the categories of HPLC separations * A compilation of HPLC terms and definitions * Tables and charts detailing solvents' properties
Maintaining And Troubleshooting Hplc System A User S Guide
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Author : Dennis J. Runser
language : en
Publisher:
Release Date : 1981
Maintaining And Troubleshooting Hplc System A User S Guide written by Dennis J. Runser and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981 with categories.
Troubleshooting Lc Systems
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Author : John W. Dolan
language : en
Publisher: Springer Science & Business Media
Release Date : 1989-03-01
Troubleshooting Lc Systems written by John W. Dolan and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-03-01 with Computers categories.
Over the last 15 years, high-performance liquid chromatogra phy (LC) has made the transition from an instrument used only by experts in research labs to a tool used for routine applications by relatively unskilled workers. With this transition have come in instrumentation and column technology. In major advances the past, the operator had to be a jack-of-all-trades, with a screw driver, soldering iron, and various wrenches as constant compan ions in the LC lab. Today, many instruments contain micropro cessors as powerful as those of mainframe computers of earlier days. With this technology has come a variety of self-diagnostic tools that allow the LC system to locate many of its own prob lems. Traditionally, well-honed LC troubleshooting skills have been a result of years of work at the bench. Today the LC system itself often can do a better job of troubleshooting than the operator can. Yet many of the problems of the past are still the major problems of today: air bubbles, check valves, detector lamps, and, of course, problems with the separation. An added pressure on the operator of today's LC system is that of productivity-the lab often cannot afford unnecessary downtime. This means that the operator has to be a troubleshooting expert, or has to have that expertise at his or her fingertips. The present book was written to provide this expertise in an easy-to-use format for users at all levels of experience.
Hplc In Enzymatic Analysis
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Author : Edward F. Rossomando
language : en
Publisher: John Wiley & Sons
Release Date : 2009-09-25
Hplc In Enzymatic Analysis written by Edward F. Rossomando and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-09-25 with Science categories.
The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.
Handbook Of Chromatography
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Author : Hamir S. Rathore
language : en
Publisher: CRC Press
Release Date : 1993-05-27
Handbook Of Chromatography written by Hamir S. Rathore and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993-05-27 with Science categories.
Handbook of Chromatography provides a detailed description of significant aspects of polycyclic aromatic hydrocarbons (PAHs). The sources, occurrence, nomenclature, and carcinogenicity of PAHs are covered, and a comprehensive record of data of sample preparation, detection, separation, determination, and characterization of PAHs by liquid chromatography is presented. The book also summarizes extraction and enrichment procedures (e.g., Soxhlet extraction, ultrasonic extraction, liquid-liquid partitioning); presents data from paper chromatography, thin-layer chromatography, high-performance thin-layer chromatography, and electrophoresis of PAHs; and discusses the methodology and applications of extrography in analyzing complex aromatic materials. Handbook of Chromatography also provides a PAHs book directory and a list of suppliers of PAHs and HPLC columns. The volume will be an essential reference for analytical chemists, environmental toxicologists, organic chemists, biomedical specialists, and biotechnicians.