Mammary Stem Cells
DOWNLOAD
Download Mammary Stem Cells PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Mammary Stem Cells book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Mammary Stem Cells
DOWNLOAD
Author : Maria dM. Vivanco
language : en
Publisher: Humana
Release Date : 2022-02-18
Mammary Stem Cells written by Maria dM. Vivanco and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-02-18 with Science categories.
This second edition provides an overview of recent developments and approaches used by researchers to investigate the properties and functions of mammary epithelial and stem cells, which will contribute to understand the heterogeneity of the mammary gland and of breast cancer. Chapters detail processes used to characterize stem cells, single cell RNA sequencing, computational methods, sophisticated imaging techniques, and a variety of model systems, among others. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols, Second Edition aims to make available protocols used to navigate the intricate behavior of mammary stem cells and to gain further knowledge to take us closer to the design of innovative strategies to prevent and treat breast cancer.
Of Microenvironments And Mammary Stem Cells
DOWNLOAD
Author :
language : en
Publisher:
Release Date : 2007
Of Microenvironments And Mammary Stem Cells written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
In most adult tissues there reside pools of stem and progenitor cells inside specialized microenvironments referred to as niches. The niche protects the stem cells from inappropriate expansion and directs their critical functions. Thus guided, stem cells are able to maintain tissue homeostasis throughout the ebb and flow of metabolic and physical demands encountered over a lifetime. Indeed, a pool of stem cells maintains mammary gland structure throughout development, and responds to the physiological demands associated with pregnancy. This review discusses how stem cells were identified in both human and mouse mammary glands; each requiring different techniques that were determined by differing biological needs and ethical constraints. These studies together create a robust portrait of mammary gland biology and identify the location of the stem cell niche, elucidate a developmental hierarchy, and suggest how the niche might be manipulated for therapeutic benefit.
Mammary Stem Cells
DOWNLOAD
Author : Maria del Mar Vivanco
language : en
Publisher: Humana
Release Date : 2015-06-04
Mammary Stem Cells written by Maria del Mar Vivanco and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-06-04 with Science categories.
The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.
Mammary Stem Cell Susceptibility To Carcinogenesis
DOWNLOAD
Author :
language : en
Publisher:
Release Date : 2005
Mammary Stem Cell Susceptibility To Carcinogenesis written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with categories.
Mammary tumor formation is thought to be the consequence of transformation of undifferentiated pluripotent stem cells. Little is known about the character of mammary gland stem cells. Examination of mammary gland development has indicated that there may be three distinct types of stem/progenitor cells (S/PC); those which only form ductal structures, those which only form alveolar structures and those which form all mammary gland structures. The goal of this work was to isolate S/PC from rat mammary glands using the recently described techniques of mammosphere formation and Hoechst dye-exclusion. Additionally, we sought to characterize the S/PC populations as to their ability to differentiate in solid matrix and their expression of certain antigens. Lastly, we aimed to determine whether the susceptibility of mammospheres (containing S/PC and transit amplifying cells TAC) to carcinogens varied compared to the intact mammary epithelial organoid (primarily TAC) . We cultured rat mammospheres and demonstrated two hallmarks of S/PC, their ability to self renew and to differentiate in solid matrix. We also isolated side-population (SP) cells which excluded Hoechst dye and reformed mammospheres. Immunostaining results were similar for organoids and mammospheres. Further work is needed to clearly assess which cell type is necessary for carcinogenesis.
Characterization Of Human Mammary Epithelial Stem Cells
DOWNLOAD
Author :
language : en
Publisher:
Release Date : 2009
Characterization Of Human Mammary Epithelial Stem Cells written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with categories.
The mammary epithelium of normal adult female mice contains stem cells with extensive in vivo regenerative and self-renewal potential. Indirect evidence has suggested that analogous cells exist in the mammary glands of adult women, and are candidate targets for the first transforming mutations that lead to the evolution of breast cancer stem cells. The objective of this grant was to determine whether these hypothesized normal human mammary stem cells might be detected and quantified by a robust and specific assay that could be used to enable the purification and phenotypic properties of these cells, and to derive information about their frequency and how they are regulated. During the 3 years of this grant, I established conditions that allow human mammary gland structures to be reproducibly generated in subrenal xenografts in highly immune deficient mice, starting with small innocula of dissociated human mammary cells. The regenerated glands are similar in morphology and cellular organization to normal human mammary glands, bounded by a basement membrane with an outer layer of myo-epithelial cells and an inner layer of polarized luminal cells that can be induced to produce milk. I also established that the presence of regenerated structures can be determined by detecting the in vitro clonogenic progenitors they contain and this endpoint can serve as an objective indicator of the presence of a primitive stem-like cell in the initial cells transplanted. This retrospective functional assay allows limiting dilution analysis of positive xenograft yields to derive mammary stem cell frequencies in differently manipulated populations. Using this approach I found the frequency of stem cells in normal human mammary tissue to be ~1 per 5000 cells and their phenotype to be CD49f+ EpCAM-/low CD31- CD45-
Cloning Of Mammary Stem Cells
DOWNLOAD
Author : Kay-Uwe Wagner
language : en
Publisher:
Release Date : 2001
Cloning Of Mammary Stem Cells written by Kay-Uwe Wagner and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with categories.
The goal of this proposal is the cloning of specific cells from the mouse mammary gland that have the capacity for self-renewal. There is overwhelming evidence that these types of cells play an important role in the origin of mammary cancer. Cloning and characterizing these cells would provide with new insights to find unique indicators for self-renewing cells that can be utilized for cell selection techniques or drug targeting. Major findings: We have identified a new mammary epithelial cell population that originates from differentiating cells during pregnancy. This epithelial population does not undergo cell death during involution and remodeling following a lactation period. We show that these cells can function as alveolar progenitors in subsequent pregnancies, and they can play an important role in functional adaptation. We have studied the growth properties of these cells in vitro, and we have clonally amplified these cells in primary mammary epithelial cell cultures. In transplantation studies, this parity-induced epithelial population shows the capacity for self-renewal and contributes significantly to ductal morphogenesis and lobulogenesis (i.e. these cells have important features of mammary stem cells). However, it is still unclear whether these cells can give rise to a!1 epithelial subtypes including myoepithelial cells.
Mammary Stem Cells
DOWNLOAD
Author : Robert B. Clarke
language : en
Publisher:
Release Date : 2003
Mammary Stem Cells written by Robert B. Clarke and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with categories.
Regulation Of Mammary Stem Cells And Breast Cancer By The Co Factor Of Lim Domains Clim Transcriptional Regulator
DOWNLOAD
Author : Michael Salmans
language : en
Publisher:
Release Date : 2014
Regulation Of Mammary Stem Cells And Breast Cancer By The Co Factor Of Lim Domains Clim Transcriptional Regulator written by Michael Salmans and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
Recent advancements in mammary gland biology demonstrate conflicting models in maintenance of basal and luminal cell compartments by either unipotent or bipotent mammary stem cells. However, the molecular mechanisms underlying control of the basal cell compartment, including stem cells, remain poorly understood. Here we explore the currently unknown transcriptional mechanisms of basal stem cell (BSC) maintenance, in addition to addressing the role of the basal cell compartment in preserving luminal cell fate and promoting development of human breast tumors of luminal origin. We discover a novel function for the Co-factor of LIM domains (Clim) transcriptional regulator in promoting mammary gland branching morphogenesis and breast tumorigenesis through maintenance of the basal stem cell population. The transcriptional networks coordinated by Clims in basal mammary epithelial cells also preserve the identity of luminal epithelial cells, demonstrating a crosstalk between these two cellular compartments. Furthermore, we correlate developmental gene expression data with human breast cancer to investigate the role of developmental pathways during the initiation and progression of breast cancer. The gene regulatory networks identified during development, including those specifically coordinated by Clims, correlate with breast cancer patient outcome, suggesting these genes play an important role in the progression of breast cancer. We also explore the role of Agr2 a putative protein disulfide isomerase (PDI) initially identified as an estrogen-responsive gene in breast cancer cell lines, in normal mammary gland development and breast cancer. While Agr2 expression in breast cancer is positively correlated with estrogen receptor (ER) expression, it is upregulated in both hormone dependent and independent carcinomas. Agr2 expression is developmentally regulated in the mammary gland, with maximum expression during late pregnancy and lactation. We show that [Subscript Agr2] facilitates normal lobuloalveolar development by regulating mammary epithelial cell proliferation. In conclusion, the estrogen-responsive Agr2, a candidate breast cancer oncogene, regulates epithelial cell proliferation and lobuloalveolar development in the mammary gland. The pro-proliferative effects of Agr2 may explain its actions in early tumorigenesis.
Mapping Putative Stem Progenitor Cells In The Mouse And Rat Mammary Glands
DOWNLOAD
Author : Diana A. Sallam
language : en
Publisher:
Release Date : 2008
Mapping Putative Stem Progenitor Cells In The Mouse And Rat Mammary Glands written by Diana A. Sallam and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with categories.
Quantitative In Situ Characterization Of A Putative Stem Cell Population In The Mouse Mammary Gland
DOWNLOAD
Author : Rodrigo Fernandez-Gonzalez
language : en
Publisher:
Release Date : 2006
Quantitative In Situ Characterization Of A Putative Stem Cell Population In The Mouse Mammary Gland written by Rodrigo Fernandez-Gonzalez and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.