Measles Virus Microform Immunoprecipitation And Monoclonal Antibody Production
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Measles Virus Microform Immunoprecipitation And Monoclonal Antibody Production
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Author : David John Paul Rafter
language : en
Publisher: National Library of Canada
Release Date : 1981
Measles Virus Microform Immunoprecipitation And Monoclonal Antibody Production written by David John Paul Rafter and has been published by National Library of Canada this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981 with Immune serums categories.
Antisera to the membrane (M) protein and to the nucleoprotein (NP) of measles virus, were prepared in rabbits. Each of the antisera was characterized by the immunoprecipitation technique and found to be monospecific. These monospecific antisera were used to determine, by indirect immunofluorescence, the cellular location of M-protein and NP during infection. The antiserum to M-protein stained the cytoplasm of Vero cells. The NP antiserum stained both the cytoplasm and nucleus. The NP was found to undergo breakdown in infected cells. This breakdown could be inhibited by aprotinin, a protease inhibitor specific for trypsin and chymotrypsin. As no protease function has been identified for the proteins of measles virus, it is believed that the proteolysis is a cellular function. The significance of proteolytic breakdown in the assembly of measles virus is discussed. Persons who receive inactivated measles vaccine and are subsequently exposed to wild type virus may experience atypical measles. It has been postulated that these individuals are susceptible to atypical measles because the inactivated vaccine fails to induce antibodies to the F-protein as detected by hemolysis-inhibition. Matched (acute and convalescent) serum samples were analyzed by the immunoprecipitation technique. Sera taken during the acute stage of the disease did not precipitate radiolabelled F-protein. Convalescent sera, which had been collected three weeks later, did precipitate the F-protein. The absence of antibodies to F confirms the earlier work obtained by hemolysis inhibition tests. These findings support the concept that atypical measles is the result of an absence of antibodies to F-protein. Once it was recognized that atypical measles appeared following the use of inactivated vaccines, an attenuated virus vaccine was introduced for widespread immunization programs. This vaccine was also used in "catch up" programs to reimmunize individuals who had previously received "killed" vaccine. The value of this "catch up" program was analyzed using matched serum samples obtained from children previously immunized with inactivated virus vaccine, who volunteered for reimmunization with the attenuated vaccine. Since in no case was reimmunization found to result in conversion to the production of antibody to F-protein, the attempt to irradicate atypical measles by this procedure should be re-evaluated. The monoclonal antibody technique was employed to obtain a series of monospecific antibodies to the measles virus proteins. In initial experiments 17 clones secreting antibody to the measles Lee strain were obtained. Of these, two clones have been established as stable cell lines. Both of these clones secrete antibody specific for the NP of the virus.
Canadiana
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Author :
language : en
Publisher:
Release Date : 1985
Canadiana written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with Canada categories.
Animal Cell Culture Techniques
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Author : Martin Clynes
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Animal Cell Culture Techniques written by Martin Clynes and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.
Tissue Culture
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Author : Paul F. Jr. Kruse
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Tissue Culture written by Paul F. Jr. Kruse and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.
Gaba
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Author : Chikako Tanaka
language : en
Publisher: Springer Science & Business Media
Release Date : 1996
Gaba written by Chikako Tanaka and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Medical categories.
Highlighting the current developments and future directions in GABA research, the ambitious aim of this topical volume is to cover GABA from the molecular mechanisms of its transmission to new targets for pharmaceutical research. Distinguished scientists at the forefront of GABA research were invited to contribute reviews on glutamate decarboxylase and autoimmunity, molecular structure and functional regulation of GABA transporters, transmitter release and GABA receptor regulation, GABA receptor subtypes and functional regulation, and GABA receptor ligands and their therapeutic application. Describing the latest advances in what has become a rapidly-evolving field, this volume will prove immensely valuable to neuroscientists, pharmacologists, biochemists, and physiologists working in the field of GABA research.
Principles Of Immunopharmacology
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Author : Frans P. Nijkamp
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-09-01
Principles Of Immunopharmacology written by Frans P. Nijkamp and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-01 with Medical categories.
This textbook provides a unique support in gaining essential knowledge on the immune response, its diagnosis and its modification by drugs and chemicals. The first section of the book, covering a basic introduction to immunology and its relevance for human disease, has been updated to accommodate new immunological concepts. The second section on immunodiagnostics has been further expanded to describe widely used molecular techniques and is followed by a systematic coverage of drugs affecting the immune system, revised to cover recent developments. The book concludes with a chapter on immunotoxicology. This third edition continues the unique format dealing with four related topics in a single volume, obviating the need to refer to several different textbooks. New aids to the reader include a two-column format, glossaries of technical terms and appendix reference tables. The emphasis on illustrations is maintained from the first edition.
Advances In Diagnosis And Management Of Cutaneous Adverse Drug Reactions
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Author : Neil H. Shear
language : en
Publisher: Springer
Release Date : 2018-10-19
Advances In Diagnosis And Management Of Cutaneous Adverse Drug Reactions written by Neil H. Shear and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-19 with Medical categories.
This resource guides prescribers, pharmacists, and regulators with an update on the recent expansion of basic and clinical knowledge that forms a framework for understanding cutaneous reactions. This understanding will lead, in turn, to better outcomes and decisions in treatment and management, both in the clinic and in the life cycle of drug development. The skin is a common target for adverse drug events and even mild rashes can be part of life-threatening syndromes. Patients and practitioners often face important decisions about therapy after a drug eruption, including treatment, cross-reactivity with future pharmaceuticals, genetic considerations and dealing with long-term sequelae after a reaction. An international team of experts and leaders in the field share their story and insights into the scientific details and relevant clinical context.
Challenges And Opportunities For Respiratory Syncytial Virus Vaccines
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Author : Larry J. Anderson
language : en
Publisher: Springer
Release Date : 2014-01-07
Challenges And Opportunities For Respiratory Syncytial Virus Vaccines written by Larry J. Anderson and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-07 with Medical categories.
Although respiratory syncytial virus (RSV) has been a high priority for vaccine development for over 50 years now, still no vaccine is available and none has yet demonstrated sufficient promise to move to licensure. The success of RSV immune prophylaxis and the availability of ever more powerful tools to study the immune response and pathogenesis of disease, combined with the ability to construct a wide variety of vaccines using different vaccine platforms, give us grounds to believe that an RSV vaccine is within reach. This book brings together in one source what is currently known about the virus: its clinical and epidemiologic features; the host response and pathogenesis of the disease; vaccines, vaccine platforms, and treatment; and animal and tissue culture models of RSV infection. It is designed to organize the critical information relevant to RSV vaccine development, facilitate the assimilation of data, and speed progress toward producing a safe and effective vaccine.
Biotechnology Prospects And Applications
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Author : R.K. Salar
language : en
Publisher: Springer Science & Business Media
Release Date : 2014-02-06
Biotechnology Prospects And Applications written by R.K. Salar and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-06 with Science categories.
Biotechnology: Prospects and Applications covers the review of recent developments in biotechnology and international authorship presents global issues that help in our understanding of the role of biotechnology in solving important scientific and societal problems for the benefit of mankind and environment. A balanced coverage of basic molecular biology and practical applications, relevant examples, colored illustrations, and contemporary applications of biotechnology provide students and researchers with the tools and basic knowledge of biotechnology. In our effort to introduce students and researchers to cutting edge techniques and applications of biotechnology, we dedicated specific chapters to such emerging areas of biotechnology as Emerging Dynamics of Brassinosteroids Research, Third generation green energy, Bioremediation, Metal Organic Frameworks: New smart materials for biological application, Bioherbicides, Biosensors, Fetal Mesenchymal Stem Cells and Animal forensics. Biotechnology: Prospects and Applications will be highly useful for students, teachers and researchers in all disciplines of life sciences, agricultural sciences, medicine, and biotechnology in universities, research stations and biotechnology companies. The book features broader aspects of the role of biotechnology in human endeavor. It also presents an overview of prospects and applications while emphasizing modern, cutting-edge, and emerging areas of biotechnology. Further, it provides the readers with a comprehensive knowledge of topics in food and agricultural biotechnology, microbial biotechnology, environmental biotechnology and animal biotechnology. The chapters have been written with special reference to the latest developments in above broader areas of biotechnology that impact the biotechnology industry. A list of references at the end of each chapter is provided for the readers to learn more about a particular topic. Typically, these references include basic research, research papers, review articles and articles from the popular literature.
Immune Infertility
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Author : Walter K.H. Krause
language : en
Publisher: Springer
Release Date : 2016-11-02
Immune Infertility written by Walter K.H. Krause and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-11-02 with Health & Fitness categories.
This book offers comprehensive coverage of both basic and clinical aspects of immune reactions responsible for infertility. It has four sections focusing on Sperm antigens, Antisperm antibodies (ASAs), Clinical impact of ASAs, and Immune contraception, and include contributions from leading experts in these fields. This new edition of the book offers a comprehensive update that reflects the very significant advances in reproductive immunology that have been achieved over the past five years, especially related to the sperm proteome, sperm-egg binding/fusion proteins, gene knockout studies, and immunocontraception. Reproductive immunology continues to be a fast-growing discipline in which new knowledge is emerging almost every day. Immune Infertility is a model source of vital and reliable information on the latest scientific developments in the field. It will be of value for clinicians, scientists, students, residents, and fellows working in reproductive biology, obstetrics and gynecology, and urology.