Methods Of Protein Analysis
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Methods For Protein Analysis
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Author : Robert A. Copeland
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Methods For Protein Analysis written by Robert A. Copeland and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
As protein science continues to become an increasingly important aspect of academic and commercial sciences and technology, the need has arisen for a ready source of laboratory protocols for the analysis and evaluation of these biological polymers. Methods for Protein Analysis presents the methods most relevant to the generalist bench scientist working with proteins. A concise yet thorough summary, it covers laboratory methods that can be reasonably performed in a standard protein laboratory, without specialized equipment or expertise. Taking a how to approach, this book examines the techniques used to answer common protein analytical questions and describes methods useful in daily laboratory work. Methods for Protein Analysis is the ideal reference for protein laboratories in academic, government and industrial settings. It is an essential benchtop manual for first-year graduate students beginning their laboratory experience as well as for chemists, biochemists, and molecular biologists in the pharmaceutical, biotechnological, food and specialty chemical industries, and for analysts concerned with the purity and structural integrity of protein. Featuring illustrations and a convenient spiral binding, this guide offers a glossary of common abbreviations and a list of suppliers for protein science.
Methods For Protein Analysis
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Author : John P. Cherry
language : en
Publisher: The American Oil Chemists Society
Release Date : 1988
Methods For Protein Analysis written by John P. Cherry and has been published by The American Oil Chemists Society this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Science categories.
Methods Of Protein Analysis
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Author : István Kerese
language : en
Publisher: Ellis Horwood
Release Date : 1984
Methods Of Protein Analysis written by István Kerese and has been published by Ellis Horwood this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Science categories.
Abstract: A reference text for food scientists, analysts, and biochemists presents a systematic, comprehensive survey of working procedures and methods for the analysis of proteins, peptides, and amino acids of varying origin and composition that can be used with equipment that generally is available in the average laboratory. Topics include: general laboratory methods for the preparation and characterization of proteinaceous materials; electrophoreticmethods (especially using polyacrylamide gels); thin-layer chromatographic methods; gel chromatography and gel filtration methods; and experimental procedures for nitrogendetermination, amino acid composition, electrophoresis, isoelectric focusing, ion-exchange chromatography, and gel filtration. Step-by-step descriptions are provided for sample preparation for analysis and problems requiring resolution are discussed.
Methods For Protein Analysis
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Author : John P. Cherry
language : en
Publisher:
Release Date : 1998
Methods For Protein Analysis written by John P. Cherry and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with categories.
Advanced Methods In Protein Microsequence Analysis
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Author : Brigitte Wittmann-Liebold
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Advanced Methods In Protein Microsequence Analysis written by Brigitte Wittmann-Liebold and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Much of the recent spectacular progress in the biological sciences can be at tributed ot the ability to isolate, analyze, and structurally characterize proteins and peptides which are present in cells and cellular organelles in only very small amounts. Recent advances in protein chemistry and in particular the application of new micromethods have led to fruitful advances in the understanding of basic cellular processes. Areas where protein-chemical studies have resulted in interest ing discoveries include the peptide hormones and their release factors, growth factors and oncogenes, bioenergetics, proton pumps and ion pumps and chan nels, topogenesis and protein secretion, molecular virology and immunology, membrane protein analysis, and receptor research. In fact, the key methods are now on hand to unravel many of the major outstanding problems of molecular biology and in particular questions of fundamental interest which relate to devel opmental biology and specificity in cell-cell interaction. In this volume we have assembled descriptions of procedures which have re cently been shown to be efficaceous for the isolation, purification, and chemical characterization of proteins and peptides that are only available in minute amounts. Emphasis is placed on well-established micromethods which have been tested and found useful in many laboratories by experienced investigators. The chapters are written by specialists, and describe a range of sensitive techniques which can be used by researchers working in laboratories with only modest resources and equipment.
Methods In Proteome And Protein Analysis
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Author : Roza Maria Kamp
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09
Methods In Proteome And Protein Analysis written by Roza Maria Kamp and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Science categories.
Following the succesful publication of "Proteome and Protein Analysis" in 2000, which was based on a former MPSA (Methods in Protein Structure Analysis) conference, Methods in Proteome and Protein Analysis presents the most interesting papers from the 14th MPSA meeting. Major topics include: protein and peptide sample preparation and separation; new reagent for protein sequence analysis; mass spectrometry in protein research; analysis of posttranslational modification; protein-protein interaction using MALDI-MS; manipulation of genome or functional compositon trap; structure-function correlation study using optical biosensors of microcolorimetrical techniques; structural proteomics as NMR or fluorescence polarization study; the classification and prediction of structure or functional sites; in silico analysis of proteins and proteomes; increasing throughput and data quality for proteomics.
Protein Analysis And Purification
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Author : Ian M. Rosenberg
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-12-01
Protein Analysis And Purification written by Ian M. Rosenberg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-01 with Science categories.
How one goes about analyzing proteins is a constantly evolving field that is no longer solely the domain of the protein biochemist. Investi gators from diverse disciplines find themselves with the unanticipated task of identifying and analyzing a protein and studying its physical properties and biochemical interactions. In most cases, the ultimate goal remains understanding the role(s) that the target protein is playing in cellular physiology. It was my intention that this manual would make the initial steps in the discovery process less time consuming and less intimidating. This book is not meant to be read from cover to cover. The expanded Table of Contents and the index should help locate what you are seeking. My aim was to provide practically oriented information that will assist the experimentalist in benchtop problem solving. The appendices are filled with diverse information gleaned from catalogs, handbooks, and manuals that are presented in a distilled fashion designed to save trips to the library and calls to technical service representatives. The user is encouraged to expand on the tables and charts to fit individual experimental situations. This second edition pays homage to the computer explosion and the various genome projects that have revolutionized how benchtop scientific research is performed. Bioinformatics and In silica science are here to stay. However, the second edition still includes recipes for preparing buffers and methods for lysing cells.
Methods In Protein Structure And Stability Analysis Conformational Stability Size Shape And Surface Of Protein Molecules
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Author : Vladimir N. Uversky
language : en
Publisher: Nova Publishers
Release Date : 2007
Methods In Protein Structure And Stability Analysis Conformational Stability Size Shape And Surface Of Protein Molecules written by Vladimir N. Uversky and has been published by Nova Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Science categories.
Protein research is a frontier field in science. Proteins are widely distributed in plants and animals and are the principal constituents of the protoplasm of all cells, and consist essentially of combinations of a-amino acids in peptide linkages. Twenty different amino acids are commonly found in proteins, and serve as enzymes, structural elements, hormones, immunoglobulins, etc., and are involved throughout the body, and in photosynthesis. This book gathers new leading-edge research from throughout the world in this exciting and exploding field of research.
Methods In Protein Sequence Analysis
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Author : Jörnvall
language : en
Publisher: Birkhäuser
Release Date : 2013-11-21
Methods In Protein Sequence Analysis written by Jörnvall and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-21 with Science categories.
Methods in protein sequence analysis constitute important fields in rapid progress. We have experienced a continuous increase in analytical sensitivity coupled with decreases in time necessary for purification and analysis. Several generations of sequencers, liquid/solid/gas-phase, have passed by and returned in other shapes during just over two decades. Similarly, the introduction of HPLC permitted an enormous leap forward in this as in other fields of biochemistry, and we now start to see new major advances in purification/analysis through capillary electrophoresis. Furthermore, progress in the field of mass spectrometry has matched that in chemical analysis and we witness continuous development, now emphasizing ion spray and other mass spectrometric approaches. In short, protein analysis has progressed in line with other developments in modern science and constitutes an indispensable, integral part of present-day molecular biology. Even the available molecular tools, in the form of proteases with different specificities, have increased in number, although we still have far to go to reach an array of "restriction proteases" like the sets of nucleases available to the molecular geneticist. Of course, conferences have been devoted to protein sequence analysis, in particular the MPSA (Methods in Protein Sequence Analysis) series, of which the 8th conference took place in Kiruna, Sweden, July 1-6 1990. Again, we witnessed much progress, saw new instruments, and experienced further interpretational insights into protein mechanisms and functions.
Amino Acid Handbook
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Author : Richard Joseph Block
language : en
Publisher:
Release Date : 1956
Amino Acid Handbook written by Richard Joseph Block and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1956 with Amino acids categories.