Methods In Proteome And Protein Analysis

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Methods In Proteome And Protein Analysis

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Author : Roza Maria Kamp
language : en
Publisher: Springer Science & Business Media
Release Date : 2004-01-23

Methods In Proteome And Protein Analysis written by Roza Maria Kamp and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-01-23 with Computers categories.

Following the succesful publication of "Proteome and Protein Analysis" in 2000, which was based on a former MPSA (Methods in Protein Structure Analysis) conference, "Methods in Proteome and Protein Analysis" presents the most interesting papers from the 14th MPSA meeting. Major topics include: X-ray crystallography, mass spectrometry or cryo-electron microscopy tomography and different experimental approaches for the study of very large multi-subunit molecular nanomaschines; development of high throughput methods for large-scale protein expression and purification and automatic data acquisition for structure determination by both X-ray diffraction and NMR spectroscopy; mechanisms of protein folding and misfolding in vitro and in vivo; protein-protein interactions; analysis of post-translational modifications; the classification, prediction of structure or functional sites, and evolution of protein folds and functions. TOC: Includes 25 chapters organized in the following parts: Structural Proteomics Proteome Analysis Structure-Function Correlations Protein-Protein Interaction Advanced Technologies Protein Sequencing and Amino Acids Analysis Bioinformatics

Methods In Proteome And Protein Analysis

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Author : Roza Maria Kamp
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09

Methods In Proteome And Protein Analysis written by Roza Maria Kamp and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Science categories.

Following the succesful publication of "Proteome and Protein Analysis" in 2000, which was based on a former MPSA (Methods in Protein Structure Analysis) conference, Methods in Proteome and Protein Analysis presents the most interesting papers from the 14th MPSA meeting. Major topics include: protein and peptide sample preparation and separation; new reagent for protein sequence analysis; mass spectrometry in protein research; analysis of posttranslational modification; protein-protein interaction using MALDI-MS; manipulation of genome or functional compositon trap; structure-function correlation study using optical biosensors of microcolorimetrical techniques; structural proteomics as NMR or fluorescence polarization study; the classification and prediction of structure or functional sites; in silico analysis of proteins and proteomes; increasing throughput and data quality for proteomics.

Methods In Proteome And Protein Analysis

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Author : Roza Maria Kamp
language : en
Publisher:
Release Date : 2014-01-15

Methods In Proteome And Protein Analysis written by Roza Maria Kamp and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-15 with categories.

Proteomic Profiling

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Author : Anton Posch
language : en
Publisher: Humana
Release Date : 2021-02-04

Proteomic Profiling written by Anton Posch and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-02-04 with Science categories.

This detailed new edition presents the latest developments of the main pillars of protein analysis, namely sample preparation, separation, and characterization. Core areas in this volume are protocols for the analysis of post-translational modifications and protein interaction partners, followed by sophisticated procedures to enrich for extracellular vesicles and organelles, along with several types of protein immuno-assays complemented by various methods for the characterization of antibodies and host-cell protein analysis. Last but not least, a few standard sample preparation protocols and recent advances concerning immuno-chemical detection of proteins are included as well. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Proteomic Profiling: Methods and Protocols, Second Edition serves as an ideal reference for students of biochemistry, biomedicine, biology, and genomics and will be an invaluable source for the experienced, practicing scientist as well.

The Proteomics Protocols Handbook

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Author : John M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-09

The Proteomics Protocols Handbook written by John M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-09 with Science categories.

Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Proteome And Protein Analysis

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Author : R.M. Kamp
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Proteome And Protein Analysis written by R.M. Kamp and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

Selected papers presented at the MPSA 98 are covering new, sensitive and rapid methods for the analysis of proteins, with special emphasis on the total cell proteins, the proteome. In addition to the experimental details, the advantages and limitations of the methodological approaches are discussed. Topics included are: Protein sequencing analysis, protein and peptide sample preparation, mass spectrometry, NMR, analysis of post-translational modifications, purification of recombinant proteins, protein-protein and protein-DNA interactions, structure prediction, modeling and protein folding, functional implications of protein domains and newly emerging methods for the investigation of the proteome, allowing to analyse the expression of genes

From Genome To Proteome

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Author : Michael J. Dunn
language : en
Publisher: John Wiley & Sons
Release Date : 2008-11-21

From Genome To Proteome written by Michael J. Dunn and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-21 with Science categories.

As research on the human, animal, plant and microbial genomes matures towards descriptive fullness, the need for understanding the proteome has clearly emerged as the next major endeavor of life sciences. Proteomics - the quantitative analysis of all proteins working in a cell at a specific time and at specific conditions - provides deep insight into the highly organized network of expression, modification and degradation of proteins. Compiled in this book are reviews and research articles which describe the recent advances and perspectives of this new field of research. The articles are grouped into the following sections: - Sample Preparation and Solubilization - Developments in Electrophoresis - Detection and Quantitation - Mass Spectrometry - Proteome Data Analysis and Management - Prokayotes and Yeast - Biological Fluids - Eukaryotic Cells and Tissue - Oncology - Plants Proteomics is a new key for the functional analysis of living systems and of equal importance for basic as well as application oriented research.

Separation Methods In Proteomics

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Author : Gary B. Smejkal
language : en
Publisher: CRC Press
Release Date : 2005-12-12

Separation Methods In Proteomics written by Gary B. Smejkal and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-12 with Medical categories.

Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomic

Handbook Of Proteomic Methods

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Author : P. Michael Conn
language : en
Publisher: Springer Science & Business Media
Release Date : 2003-07-10

Handbook Of Proteomic Methods written by P. Michael Conn and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-07-10 with Science categories.

A compendium of thirty-four powerful techniques for identifying and analyzing the diversity of proteins expressed in cells. Thee readily reproducible proteomic methods range from general to specific techniques, and include methods for data analysis, posttranslational modification, and its variants and isoforms. Additional methods demonstrate the application of proteomics to the discovery of serological tumor markers, to identifying the determinants of sensitivity to antitumor drugs, and to specialized fields, such as endocrinology, plant biology, nephrology, and urology.

Proteomics In Nephrology

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Author : V. Thongboonkerd
language : en
Publisher: Karger Medical and Scientific Publishers
Release Date : 2004-07

Proteomics In Nephrology written by V. Thongboonkerd and has been published by Karger Medical and Scientific Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-07 with Medical categories.

The novel discipline of proteomics has experienced a rapid growth in the recent past and has a great potential for the future. Involving several fields, it offers various advantages: The study of proteins on a genomic scale allows to examine a large number of them simultaneously. Moreover, proteomic analysis provides information about post-translational modifications and functions of gene products or proteins that cannot be obtained by genomic analysis. The knowledge thus gained is also important for a better understanding of renal physiology and pathophysiology, to identify disease biomarkers, and to develop new target therapies. This book is the first one that applies the science of proteomics to nephrology: It sets out to give an overview of the discipline, providing detailed background information and outlining scope, advantages, and limitations of proteomics. The second section reviews principles of commonly used proteomic techniques, including sample preparation, two-dimensional gel electrophoresis, various types of mass spectrometry, expression proteomics, quantitative proteomics, practical bioinformatics, and clinical proteomics. In the last section, examples and perspectives are provided of how proteomics can be applied to various fields in nephrology, including nephrotoxicity, sodium transport, diabetic nephropathy, renal Fanconi syndrome, IgA nephropathy, physiology of podocytes and mesangial cells, hypertension, urological malignancies, and dialysis. The publication ends with a discussion of biomarker discovery in glomerular diseases and drug discovery. Written by acclaimed experts in both proteomics and nephrology, this truly remarkable book is essential reading for students, residents, fellows, scientists, and nephrologists who are interested in or devoted to research, as well as for general health professionals and clinicians.