Microinjection
DOWNLOAD
Download Microinjection PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Microinjection book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Microinjection
DOWNLOAD
Author : Juan Carlos Lacal
language : en
Publisher: Birkhäuser
Release Date : 2013-12-01
Microinjection written by Juan Carlos Lacal and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-01 with Science categories.
This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system.
Microinjection And Organelle Transplantation Techniques
DOWNLOAD
Author : Julio E. Celis
language : en
Publisher:
Release Date : 1986
Microinjection And Organelle Transplantation Techniques written by Julio E. Celis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with Medical categories.
Microinjection of tissue culture cells using glass; Transformation capacity of SV40 DNA and subgenomic DNA fragments; Modulation of cellular activities via microinjection into living cells; Studies of retroviral packagin;. Erythrocyte-mediated transfer: methods; Erythrocyte-mediated transfer: applications; Liposomes for microinjection of molecules into cells; Liposomes in drug targeting; Liposomes as carriers for gene transfer in vivo; Transformation of mammalian cells with DNA using the calcium technique; The somatic cell as a tes tube: comparison between various transfer techniques.
Microinjection And Transgenesis
DOWNLOAD
Author : Angel Cid-Arregui
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Microinjection And Transgenesis written by Angel Cid-Arregui and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics.
Microinjection
DOWNLOAD
Author : David J. Carroll
language : en
Publisher: Humana Press
Release Date : 2016-08-23
Microinjection written by David J. Carroll and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.
Biotechnology Applications Of Microinjection Microscopic Imaging And Fluorescence
DOWNLOAD
Author : Peter H. Bach
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Biotechnology Applications Of Microinjection Microscopic Imaging And Fluorescence written by Peter H. Bach and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Individual cells behave in surpnsmg ways that cannot be deduced from the averaged results of an organ as assessed by the use of conventional biochemical methods. Thus multicellular plant and animals systems are being investigated by an increasing array of histochemical and cytochemical techniques based on general chemical or specific immunological interactions to identify structural materials and to assess biological activities. In recent years there has been an increasing range of fluorescent probes, along with advanced computerised imaging and analysis techniques, which allows the behaviour of individual living cells to be followed in considerable detail. The parallel use of microinjection, microelectrodes and patch-clamping provides additional information about cells and their responses. Recombinant DNA technology has highlighted the desirability and the power of microinjecting defined materials into specific cells and so manipulating their fundamental biochemistry. New hypotheses are being tested which will form the cornerstone of future developments across the whole spectrum of biotechnology. The First European Workshop on Biotechnology Applications of Microinjection, Microscopic Imaging and Fluorescence was run at the University of East London, U.K, 21st-24th April, 1992 with the objective of bringing together a diverse group of individuals who were using these state-of-the-art applications for biotechnological exploration. A novel feature of the meeting was paiticipation by instrument manufacturers in the programme: there were hands-on workshops (where living cells could be examined), combined with the poster sessions.
Microinjection
DOWNLOAD
Author : Chengyu Liu
language : en
Publisher: Humana Press
Release Date : 2018-10-18
Microinjection written by Chengyu Liu and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-18 with Science categories.
This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations.
Electrophysiology And Microinjection
DOWNLOAD
Author : P. Michael Conn
language : en
Publisher: Elsevier
Release Date : 2016-07-02
Electrophysiology And Microinjection written by P. Michael Conn and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-07-02 with Science categories.
Electrical activity is central to the functioning and nature of neural cells. Every effort has been made in this volume to provide representative examples of useful techniques that have made this area a productive component of the neurosciences. Convenient bench-top format**Methods presented for easy adaptation to new systems**Comprehensive protocols included for**Cell recording: from conscious and freely behaving animals, measurement of calcium currents, and multiple electrodes**Voltage clamping and voltammetry: apparatus and applications**Specific ion channels and receptors: expression in oocytes, single-cell secretion, purification of calcium channel drug receptors**Special electrodes and other equipment: movable electrodes, microelectrodes, multiple electrodes, en passant recording, microincubators for optical studies**Special preparations: multicompartment cell cultures, grease-gap methods, perforated path techniques, giant proteoliposomes for reconstitution studies, microinjection**Data analysis and reduction: digital filtering on a personal computer, filtering on a personal computer, fitting of single-channel dwell time distributions, discrimination of kinetic models, voltage noise analysis, list-oriented analysis of single-channel systems
Microinjection
DOWNLOAD
Author : J. C. Lacal
language : en
Publisher:
Release Date : 1996-10-01
Microinjection written by J. C. Lacal and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-10-01 with categories.
Transgenic Xenopus
DOWNLOAD
Author : Shlomo Seidman
language : en
Publisher: Springer Science & Business Media
Release Date : 1997
Transgenic Xenopus written by Shlomo Seidman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.
Annotation Explains techniques for microinjecting foreign genes into transgenic embryos of frogs and the information such research can provide about the role and spatiotemporal regulation of nervous system proteins during vertebrate development. Explains the basic scientific background and protocols, development, in vitro fertilization and microinjection, and a variety of biochemical and histochemical techniques for analyzing the results of the experiment. Also presents extensive data gathered by the approach. Annotation c. by Book News, Inc., Portland, Or.
Electrophysiology And Microinjection
DOWNLOAD
Author : P. Michael Conn
language : en
Publisher:
Release Date : 1981
Electrophysiology And Microinjection written by P. Michael Conn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981 with categories.