Microinjection And Transgenesis
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Microinjection And Transgenesis
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Author : Angel Cid-Arregui
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Microinjection And Transgenesis written by Angel Cid-Arregui and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics.
Microinjection And Transgenesis Strategi
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Author :
language : en
Publisher:
Release Date : 1997
Microinjection And Transgenesis Strategi written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
Transgenic Ascidians
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Author : Yasunori Sasakura
language : en
Publisher: Springer
Release Date : 2018-03-14
Transgenic Ascidians written by Yasunori Sasakura and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-03-14 with Science categories.
This book comprehensively describes the transgenesis techniques and applied experimental methods in ascidians including enthusiastically developed original devices in addition to concrete examples of developmental biology studies. Ascidians have been one of the most important model animals in developmental biology for studying molecular and cellular processes underlying formation of the chordate body plan. Transgenic techniques such as microinjection, electropolation, cis-element analysis and application, and TALENs and CRISPR/Cas9 have been developed in ascidians for more than 20 years, and now many applied methods, some of which are unique in ascidians, have been accumulated. Those extensive technological innovations, such as cell isolation, cell labeling, germ-line transformation, marker transgenic lines, and the experimental systems for studying notochord formation and nervous system, are exceptional particularly in marine invertebrates. This book is useful for ascidian researchers to quickly access the techniques in which they are interested as well as to compare each technology to become familiar with specialized tips, and for biologists of other organisms to learn the unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily understandable descriptions of techniques, the book will inspire ascidian specialists to improve their techniques, encourage anyone wanting to begin studying ascidians, and enable readers to immediately apply the techniques to the organisms they research.
Microinjection
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Author : David J. Carroll
language : en
Publisher: Humana Press
Release Date : 2016-08-23
Microinjection written by David J. Carroll and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.
Transgenic Animals
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Author : Louis-Marie Houdebine
language : en
Publisher: CRC Press
Release Date : 2022-04-19
Transgenic Animals written by Louis-Marie Houdebine and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-04-19 with Nature categories.
During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues. Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics. Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.
Transgenic Animal Technology
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Author : Carl A. Pinkert
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Transgenic Animal Technology written by Carl A. Pinkert and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
A transgenic animal is one that is genetically modified to carry genes from another species. Transgenic species can be raised to carry potentially useful genes from a variety of species. While the topics of genetic engineering and cloning are controversial, the reality is that these technologies offer tremendous benefits to society - from offering a framework for developing and screening medical therapies, to enhancing the safety and nutrition of the food we eat. One potential application of research into transgenic animal technology is the creation of domestic animals genetically designed to express a certain human disease and therefore serve as models for the study and treatment of human illnesses. Although many mouse models of human diseases are available today, such models in large domestic animals physiologically more similar to humans are sparse and critically needed. Further research in this field will undoubtedly uncover many more direct and indirect benefits of this technology. Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols New section on analysis
Advanced Protocols For Animal Transgenesis
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Author : Shirley Pease
language : en
Publisher: Springer Nature
Release Date : 2011-08-30
Advanced Protocols For Animal Transgenesis written by Shirley Pease and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-30 with Science categories.
This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.
Microinjection
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Author : Chengyu Liu
language : en
Publisher: Humana Press
Release Date : 2018-10-18
Microinjection written by Chengyu Liu and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-18 with Science categories.
This detailed book explores how microinjection will be used in the foreseeable future, not only for generating animal models for biomedical research but also for changing economically or ecologically important species that can broadly impact our society in general. The opening half of the book focuses on methods for generating mouse models, as they are still the most popular in genome engineering research, while the second half examines gene-editing in a variety of other species, opened up by the developments in ZFN, TALEN, and CRISPR techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microinjection: Methods and Protocols serves as an ideal guide for researchers looking to take advantage of the breakthrough technologies in gene-editing and embryo micromanipulations.
Welfare Aspects Of Transgenic Animals
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Author : L.F.M. van Zutphen
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Welfare Aspects Of Transgenic Animals written by L.F.M. van Zutphen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
v Preface The quality of animal models used in biomedical research has increased substantially since the first successful microinjection of foreign DNA frag ments into a mouse zygote in the early 1980s. New well-defined animal models, developed either by micro injection techniques or by homologous recombination in embryonic stem (ES) cells, have become widely available and several classical animal models have now been replaced by these transgenic models. Transgenic animals also fulfill increasingly important roles in other fields, like livestock production or in biopharming. The potential benefits of this technology are overwhelming. It is not surprising that many research groups have rushed to jump on this wagon of scientific progress. However, no matter how important these developments are, they can not relieve us of the moral obligations towards the animals. It is generally recognized that animal use is allowed only if the harmful effects are out weighed by the potential benefits of the experimental results. But what are the harmful effects? What is the impact of trans genesis on the well-being of animals? In order to gain more insight into this aspect of trans genesis the European Commission (DGXII) has funded a 12-month project with the aim of col lecting information on the production and use of transgenic animals in EU Member States and, more specifically, to obtain information as to what extent specific welfare problems may arise and whether or not studies are being performed on the incidence of welfare problems.
Mammary Gland Transgenesis Therapeutic Protein Production
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Author : Fidel O. Castro
language : en
Publisher: Springer Science & Business Media
Release Date : 1998-02-12
Mammary Gland Transgenesis Therapeutic Protein Production written by Fidel O. Castro and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-02-12 with Science categories.
This book presents the latest in mammary gland transgenesis, the exploitation of transgenic technology for the production of therapeutic proteins by routine or conventional methods. Following a section with an overview of all relevant methodologies, readers will find relevant information on the regulation of milk gene expression and bioreactor species such as cattle, rabbits and pigs.