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Microinjection And Transgenesis

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Microinjection And Transgenesis

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Author : Angel Cid-Arregui
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Microinjection And Transgenesis written by Angel Cid-Arregui and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics.

Transgenic Animal Technology

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Author : Carl A. Pinkert
language : en
Publisher: Newnes
Release Date : 2014-06-17

Transgenic Animal Technology written by Carl A. Pinkert and has been published by Newnes this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-17 with Science categories.

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. - Contains a comprehensive collection of transgenic animal and gene transfer methods - Discusses background and introduction to techniques and animal systems - Teaches practical step-by-step protocols - Fully revised with updates to reflect state-of-the-art technology and associated changes to date

Safety Of Genetically Engineered Foods

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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 2004-08-08

Safety Of Genetically Engineered Foods written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-08-08 with Science categories.

Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Advanced Protocols For Animal Transgenesis

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Author : Shirley Pease
language : en
Publisher: Springer Nature
Release Date : 2011-08-30

Advanced Protocols For Animal Transgenesis written by Shirley Pease and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-30 with Science categories.

This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Transgenic Animals

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Author : Louis-Marie Houdebine
language : en
Publisher: CRC Press
Release Date : 2022-04-19

Transgenic Animals written by Louis-Marie Houdebine and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-04-19 with Nature categories.

During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues. Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics. Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.

Mammary Gland Transgenesis Therapeutic Protein Production

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Author : Fidel O. Castro
language : en
Publisher: Springer Science & Business Media
Release Date : 1998-02-12

Mammary Gland Transgenesis Therapeutic Protein Production written by Fidel O. Castro and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-02-12 with Science categories.

This book presents the latest in mammary gland transgenesis, the exploitation of transgenic technology for the production of therapeutic proteins by routine or conventional methods. Following a section with an overview of all relevant methodologies, readers will find relevant information on the regulation of milk gene expression and bioreactor species such as cattle, rabbits and pigs.

Animal Transgenesis And Cloning

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Author : Keywon Frazier
language : en
Publisher: Scientific e-Resources
Release Date : 2018-09-27

Animal Transgenesis And Cloning written by Keywon Frazier and has been published by Scientific e-Resources this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-09-27 with categories.

Transgenic methodologies continue to evolve and have dramatically influenced a cross section of disciplines. They are recognized as instrumental in expanding our understanding of gene expression, regulation and function. This book covers the aspects of gene transfer in animals-from molecular methods to whole animal considerations across a host of species. The book starts with an introduction of what are transgenic animals. Chapter 1 methods and applications related to transgenic application. Chapter 2 describes the Use of Transgenic Animals in Biotechnology as Prospects and Problems. Chapter 3 study about Transgenic Animals in Agriculture. Chapter 4 depicts about the Gene Replacement and Transgenic Animals. This chapter give insight on Specific Sites in Cloned Genes Can Be Altered in Vitro and DNA that can be transferred into Eukaryotic Cells in Various Ways. Chapter 5 discuss about basics of cloning. Chapter 6 tells about the Reproductive Cloning. Chapter 7 tells about the Cloning of Domestic Animals. Chapter 8 depicts about the Surface Epigenetic Reprogramming. Chapters 9 devoted to Animal Health Risks. This chapter focus on the critical biological systems approach to the analysis of clone animal. Chapter 10 describes the development of the Risk Assessment Methodology required for cloning.

The Mouse In Biomedical Research

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Author :
language : en
Publisher: Elsevier
Release Date : 2006-12-04

The Mouse In Biomedical Research written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-12-04 with Science categories.

History, Wild Mice, and Genetics, the first volume in the four volume set, The Mouse in Biomedical Research, provides information about the history, biology and genomics of the laboratory mouse (Mus musculus), as well as basic information on maintenance and use of mouse stocks. Mouse origins and relationships are covered in chapters on history, evolutionary taxonomy and wild mice. Genetics and genomics of the mouse are covered in chapters on genetic nomenclature, gene mapping, cytogenetics and the molecular organization of the mouse genome. Maintenance of laboratory mice is described in chapters on breeding systems for various types of strains and stocks and genetic monitoring. Use of the mouse as a model system for basic biomedical research is described in chapters on chemical mutagenesis, gene trapping, pharmacogenetics and embryo manipulation. The information in Volume 1 serves as a primer for scientists new to the field of mouse research.

Introduction To Pharmaceutical Biotechnology

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Author : Saurabh Bhatia
language : en
Publisher: IOP Publishing Limited
Release Date : 2024-07-09

Introduction To Pharmaceutical Biotechnology written by Saurabh Bhatia and has been published by IOP Publishing Limited this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-07-09 with Medical categories.

The recent advancements in biotechnology, particularly in post-COVID era is accelerating the pace of research and development in all areas of biological sciences. Thus, the aim & scope of this book is to clearly illustrate ideas on diverse ongoing cutting-edge advancements in the field biotechnology and current scenario across a wide subject spectrum.

Transgenic Animals

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Author : Louis-Marie Houdebine
language : en
Publisher: CRC Press
Release Date : 1997-05-22

Microinjection And Transgenesis

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