Advanced Protocols For Animal Transgenesis
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Advanced Protocols For Animal Transgenesis
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Author : Shirley Pease
language : en
Publisher: Springer Nature
Release Date : 2011-08-30
Advanced Protocols For Animal Transgenesis written by Shirley Pease and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-30 with Science categories.
This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.
Advanced Protocols For Animal Transgenesis
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Author : Shirley Pease
language : en
Publisher: Springer
Release Date : 2016-08-23
Advanced Protocols For Animal Transgenesis written by Shirley Pease and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.
Transgenic Animal Technology
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Author : Carl A. Pinkert
language : en
Publisher: Newnes
Release Date : 2014-06-17
Transgenic Animal Technology written by Carl A. Pinkert and has been published by Newnes this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-17 with Science categories.
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. - Contains a comprehensive collection of transgenic animal and gene transfer methods - Discusses background and introduction to techniques and animal systems - Teaches practical step-by-step protocols - Fully revised with updates to reflect state-of-the-art technology and associated changes to date
Transgenesis
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Author : Thomas L. Saunders
language : en
Publisher: Springer Nature
Release Date : 2023-03-30
Transgenesis written by Thomas L. Saunders and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-03-30 with Science categories.
This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research. Future advances in biomedical research will benefit greatly from the use of precise gene targeting of transgenes in the genome as CRISPR technology supersedes earlier methods that relied on random transgene integration, which this collection reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Transgenesis: Methods and Protocols serves as an ideal guide for researchers working with or working on genetically modified models.
Biotechnology For Sustainable Agriculture
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Author : Ram Lakhan Singh
language : en
Publisher: Woodhead Publishing
Release Date : 2017-09-12
Biotechnology For Sustainable Agriculture written by Ram Lakhan Singh and has been published by Woodhead Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-09-12 with Technology & Engineering categories.
Biotechnology for Sustainable Agriculture: Emerging Approaches and Strategies is an outstanding collection of current research that integrates basic and advanced concepts of agricultural biotechnology with future development prospects. Using biotechnology with sustainable agriculture effectively contributes to gains in agricultural productivity, enhanced food security, reduced poverty and malnutrition, and more ecologically sustainable means of food production. Written by a panel of experts, this book is unique in its coverage of the broad area of biotechnology for sustainable agriculture. It includes intriguing topics and discussions of areas such as recombinant DNA technology and genetic engineering. - Identifies and explores biotechnological tools to enhance sustainability - Encompasses plant and microbial biotechnology, nanotechnology and genetic engineering - Focuses on plant biotechnology and crop improvement to increase yield and resilience - Summarizes the impact of climate change on agriculture, fisheries and livestock
Emerging Issues In Climate Smart Livestock Production
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Author : Sukanta Mondal
language : en
Publisher: Academic Press
Release Date : 2021-12-03
Emerging Issues In Climate Smart Livestock Production written by Sukanta Mondal and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-12-03 with Technology & Engineering categories.
Emerging Issues in Climate Smart Livestock Production: Biological Tools and Techniques furnishes a detailed reference on livestock sustainability and the role of biotechnology for creating more sustainable livestock production systems. The book is a collection of scientific techniques, including genetic engineering used to modify and improve animals, fishes, and microorganisms for human benefit. The book is particularly attractive for scientists, researchers, students, educators, and professionals in agriculture, veterinary, and biotechnology science. This book promotes several biotechnological approaches that can easily be evaluated in the field for quality assurance programs beneficial to producing livestock products and overall public health. Biotechnology has the potential to improve the productivity of animals via increased growth, carcass quality and reproduction, improved nutrition and feed utilization, improved food quality and safety, improved animal health and welfare, and reduced waste through more efficient utilization of resources. - Identifies and explores biotechnological approaches for sustainable livestock and fish production - Focuses on strategies for enhancing livestock and fishery productivity and sustainability - Presents the latest research on modern methods and technologies
Protocols In Advanced Genomics And Allied Techniques
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Author : Aruna Pal
language : en
Publisher: Springer Nature
Release Date : 2021-11-14
Protocols In Advanced Genomics And Allied Techniques written by Aruna Pal and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-11-14 with Science categories.
This laboratory manual includes the latest tools and techniques involved in genomic research. It starts with an introductory chapter on genomics and the various tools and applications involved. The initial chapters present protocols for basic techniques such as DNA isolation, electrophoresis, PCR, cDNA synthesis etc. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc. Each topic includes a brief description, information on the principles involved, materials & methods, protocol, and expected results, with diagrams and graphs. All protocols are presented in a very lucid and precise way, to make it easy for readers to follow and replicate them.
Rodent Quality Control Genes And Bugs
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Author : Fernando Benavides
language : en
Publisher: Springer Nature
Release Date : 2024-08-12
Rodent Quality Control Genes And Bugs written by Fernando Benavides and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-08-12 with Medical categories.
This book shows how and why quality assurance programmes should be implemented in laboratory rodent facilities to monitor animal health and genetic status. It covers common practice to assure the desired level of microbiological and genetic surveillance of both genetically standardized and genetically altered laboratory mice and rats. In particular, the book introduces readers to the standardized genetic nomenclature and provides step-by-step protocols for genetic monitoring of the animals. International standards of health monitoring programs are discussed and statistical aspects thereof are explored. Furthermore, the book reviews common infectious diseases in laboratory rodents and guides the reader in monitoring the animals’ health status through application of current diagnostic methods. This book adresses researchers, technicians and zookeepers working in laboratory rodent facilities.
The Guide To Investigation Of Mouse Pregnancy
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Author : B. Anne Croy
language : en
Publisher: Academic Press
Release Date : 2013-12-09
The Guide To Investigation Of Mouse Pregnancy written by B. Anne Croy and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-09 with Science categories.
The Guide to Investigation of Mouse Pregnancy is the first publication to cover the mouse placenta or the angiogenic tree the mother develops to support the placenta. This much-needed resource covers monitoring of the cardiovascular system, gestational programming of chronic adult disease, epigenetic regulation, gene imprinting, and stem cells. Offering detailed and integrated information on how drugs, biologics, stress, and manipulations impact pregnancy in the mouse model, this reference highlights techniques used to analyze mouse pregnancy. Joining the ranks of much referenced mouse resources, The Guide to Investigation of Mouse Pregnancy is the only manual providing needed content on pregnancy in animal models for translational medicine and research. - Provides instruction on how to collect pre-clinical data on pregnancy in mouse models for eventual use in human applications - Describes the angiogenic tree the mother's uterus develops to support pregnancy and the monitoring of pregnancy-induced cardiovascular changes - Educates readers on placental cell lineages, decidual development including immune cells, epigenetic regulation, gene imprinting, stem cells, birth and lactation - Discusses how stress, environmental toxicants and other manipulations impact upon placental function and pregnancy success
Basic And Applied Bone Biology
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Author : David B. Burr
language : en
Publisher: Academic Press
Release Date : 2019-02-20
Basic And Applied Bone Biology written by David B. Burr and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-20 with Reference categories.
Basic and Applied Bone Biology, Second Edition, provides an overview of skeletal biology, from the molecular level, to the organ level, including cellular control, interaction and response, adaptive responses to various external stimuli, and the interaction of the skeletal system with other metabolic processes in the body. The book includes chapters that address how the skeleton can be evaluated through the use of various imaging technologies, biomechanical testing, histomorphometric analysis, and the use of genetically-modified animal models. Each chapter delves deep into the important details of topics covered to provide a solid understanding of the basics of bone biology. Bone biology researchers who also train undergraduate and graduate students in the lab will use this book constantly to orient new students on the basics of the field and as a background reference for many of the technical aspects of qualification in bone biology (e.g., mechanics, histomorphometry, genetic modification, biochemistry, etc.). - Presents an in-depth overview of skeletal biology, from molecular to organ level - Offers refresher level content for clinicians or researchers outside their areas of expertise - Includes updated and complete references - Incorporates expanded study questions at the end of each chapter for further exploration - Covers topics relevant to a modern course in skeletal biology