Transgenesis
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Transgenesis
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Author : Thomas L. Saunders
language : en
Publisher: Springer Nature
Release Date : 2023-03-30
Transgenesis written by Thomas L. Saunders and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-03-30 with Science categories.
This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research. Future advances in biomedical research will benefit greatly from the use of precise gene targeting of transgenes in the genome as CRISPR technology supersedes earlier methods that relied on random transgene integration, which this collection reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Transgenesis: Methods and Protocols serves as an ideal guide for researchers working with or working on genetically modified models.
Transgenic Animals
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Author : Louis-Marie Houdebine
language : en
Publisher: CRC Press
Release Date : 2022-04-19
Transgenic Animals written by Louis-Marie Houdebine and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-04-19 with Nature categories.
During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues. Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics. Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.
Advanced Protocols For Animal Transgenesis
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Author : Shirley Pease
language : en
Publisher: Springer Nature
Release Date : 2011-08-30
Advanced Protocols For Animal Transgenesis written by Shirley Pease and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-30 with Science categories.
This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.
Transgenic Mouse Methods And Protocols
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Author : Marten H. Hofker
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Transgenic Mouse Methods And Protocols written by Marten H. Hofker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularlyfor generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented.
Transgenesis And Targeted Mutagenesis In Immunology
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Author : Horst Bluethmann
language : en
Publisher: Academic Press
Release Date : 1994-06-16
Transgenesis And Targeted Mutagenesis In Immunology written by Horst Bluethmann and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-06-16 with Medical categories.
A laboratory guide to the study of transgenesis and targetted mutagenesis of laboratory mice. The book also addresses the use of mouse models in studying the immune system, focuses on gene distribution in embryonic stem cells and analyzes the in vivo functional loss of embryonic cells.
Animal Transgenesis And Cloning
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Author : Louis-Marie Houdebine
language : en
Publisher: Wiley
Release Date : 2003-04-02
Animal Transgenesis And Cloning written by Louis-Marie Houdebine and has been published by Wiley this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-04-02 with Science categories.
Animal Transgenesis and Cloning is a concise, balanced introduction to this dynamic subject, covering key issues and current techniques currently used in animal transgenesis and cloning. Whilst providing the reader with the essentials of the subject, from the molecular basis of gene structure and function to therapeutic gene cloning in humans, social and ethical implications of this important area of research are also considered. Written in a clear, accessible style, the book starts with an introduction to key molecular biology techniques and in particular, considers techniques used specifically for cloning animals and generating transgenic animals. Later chapters examine the diverse theoretical, technical and ethical issues raised by cloning and transgenesis in both animals and humans. Animal Transgenesis and Cloning: * Is an accessible, concise introduction to this dynamic field * Discusses biological, technical and ethical issues related to cloning and transgenesis * Provides an up-to-date account of the latest research methods and applications. Reviews 'This is a proposal for a book translation in a field that is clearly of importance and very topical. The idea of the book, from gene to transgenic animal, to its uses and abuses is sound and logical ...... It is probable that I would suggest (using) a book similar to the one proposed' Dr Janet Smith, University of Birmingham. 'Chapter one was a tour de force! Very concise and admirably clear ...... I was particularly impressed with the author's 2-page summary of eukaryotic gene regulation, which is no mean achievment!' Dr David de Pomerai, University of Nottingham. '...... I felt that this is a well written, topical book. It covers a lot of ground ...... in my opinion the author manages to provide a satisfying amount of detail and a well focussed treatment of each topic. I like the logic of the book, the fact that it pulls together all of the issues into one readable and manageable text' Dr Brendan Curran, Queen Mary, University of London.
Animal Biotechnology
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Author : Manjula Shenoy
language : en
Publisher: Firewall Media
Release Date : 2007
Animal Biotechnology written by Manjula Shenoy and has been published by Firewall Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Animal biotechnology categories.
Microinjection And Transgenesis
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Author : Angel Cid-Arregui
language : en
Publisher: Springer
Release Date : 1998
Microinjection And Transgenesis written by Angel Cid-Arregui and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Medical categories.
The technique of microinjection is used to tranfer biological materials - RNA, proteins, oligonucleotides and especially cloned genes - into cultured somatic cells or embryos. Transgenic cells or animals - mice, rabbits or insects - allow the study of a wide range of cellular processes, such as gene expression, signal transduction or the cytoskeleton. More than 30 protocols on microinjection experiments and transgenesis studies developed in renowned laboratories are described in this manual. Special topics are the generation of transgenic mice using YAC, ligand-dependent site-specific recombination in embryonic stem cells, morula aggregation to generate germline chimeras as well as the analysis of injected cells.
Animal Transgenesis And Cloning
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Author : Louis-Marie Houdebine
language : en
Publisher: Wiley-Blackwell
Release Date : 2003-04-02
Animal Transgenesis And Cloning written by Louis-Marie Houdebine and has been published by Wiley-Blackwell this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-04-02 with Medical categories.
Designed to be a concise, balanced introduction to this dynamic subject, covering key issues and current techniques currently used in animal transgenesis and cloning. Provides the essentials of the subject, from the molecular basis of gene structure and function to therapeutic gene cloning in humans. Social and ethical implications of this important area of research are also considered.
Transgenic Ascidians
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Author : Yasunori Sasakura
language : en
Publisher: Springer
Release Date : 2018-03-14
Transgenic Ascidians written by Yasunori Sasakura and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-03-14 with Science categories.
This book comprehensively describes the transgenesis techniques and applied experimental methods in ascidians including enthusiastically developed original devices in addition to concrete examples of developmental biology studies. Ascidians have been one of the most important model animals in developmental biology for studying molecular and cellular processes underlying formation of the chordate body plan. Transgenic techniques such as microinjection, electropolation, cis-element analysis and application, and TALENs and CRISPR/Cas9 have been developed in ascidians for more than 20 years, and now many applied methods, some of which are unique in ascidians, have been accumulated. Those extensive technological innovations, such as cell isolation, cell labeling, germ-line transformation, marker transgenic lines, and the experimental systems for studying notochord formation and nervous system, are exceptional particularly in marine invertebrates. This book is useful for ascidian researchers to quickly access the techniques in which they are interested as well as to compare each technology to become familiar with specialized tips, and for biologists of other organisms to learn the unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily understandable descriptions of techniques, the book will inspire ascidian specialists to improve their techniques, encourage anyone wanting to begin studying ascidians, and enable readers to immediately apply the techniques to the organisms they research.