Mouse Cell Culture
DOWNLOAD
Download Mouse Cell Culture PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Mouse Cell Culture book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Mouse Cell Culture
DOWNLOAD
Author : Andrew Ward
language : en
Publisher: Humana
Release Date : 2016-08-23
Mouse Cell Culture written by Andrew Ward and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.
Comparing Cell Culture And Mouse Assays For Measuring Infectivity Of Cryptosporidium
DOWNLOAD
Author : Paul A. Rochelle
language : en
Publisher: IWA Publishing
Release Date : 2005-06-30
Comparing Cell Culture And Mouse Assays For Measuring Infectivity Of Cryptosporidium written by Paul A. Rochelle and has been published by IWA Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-06-30 with Science categories.
Cell culture techniques are routinely used for measuring the infectivity of a wide range of human pathogens. A variety of different cell culture systems and detection methodologies have been applied to Cryptosporidium parvum. However, the correlation between cell culture methods and animal infectivity assays has not been thoroughly investigated. Although many cell culture methods have been developed for C. parvum, it has not been proven that infectivity in cell culture is a good indicator of the ability of oocysts to cause infections in animals. The objective of this research was to compare in-vitro cell culture methods with a mouse assay for measuring infectivity of C. parvum oocysts. The specific objectives were to (1) compare the dose response and sensitivity of cell culture and mouse assays with multiple isolates; (2) compare infectivity methods with oocysts exposed to environmental water samples; (3) determine the reproducibility and variability of the methods; and (4) compare cell culture and animal assays for assessing ozone and UV disinfection.For untreated oocysts, challenge doses were enumerated by flow cytometry. Dose response curves were constructed by regression analysis of oocyst dose against a logistic transformation of the proportional infectivity and the 50% infectious doses for each isolate were calculated by solving the regression for a logit value of zero. Infections in CD-1 mice were detected by microscopy following staining with hematoxylin and eosin. Infection in HCT-8 and Caco-2 cells was detected by C. parvum-specific RT-PCR. In MDCK cells, infection was detected using immunofluorescence. For disinfection studies, oocysts were exposed to UV using a medium-pressure, collimated beam apparatus and inactivation was measured as the difference in ID50 of unexposed and UV-exposed oocysts. Oocysts were exposed to ozone using batch, semi-batch, and single continuously stirred tank reactors at 1, 5, and 15°C.This investigation demonstrated that in-vitro cell culture was equivalent with a mouse assay for measuring infectivity of untreated C. parvum oocysts and should therefore be considered a practical alternative for assessing the potential of oocysts to cause infection. However, the high levels of variability displayed by mouse and cell culture methods indicated that infectivity and disinfection experiments should be limited to discerning relatively large differences. Of the three cell culture assays, the HCT-8/RT-PCR method displayed the closest agreement with the CD-1 mouse assay. C. hominis was infectious in HCT-8 cells but did not infect mice. Similar results were obtained with CD-1 mice and HCT-8 cells for measuring infectivity of oocysts that had been exposed to environmental water for 35 days. There was also very good agreement between HCT-8 cell culture and CD-1 mouse assays for measuring UV inactivation of C. parvum. A medium-pressure UV dosage of 5.6 mJ/cm2 resulted in 2-log10 inactivation. The shapes of ozone inactivation curves were generally the same for mouse and cell culture derived data although the CD-1 mouse assay typically generated 0.5 to 1-log10 higher levels of inactivation than HCT-8 cells. In addition, there was a stimulatory response in oocysts exposed to ozone below 20 mg.min/L when assayed by HCT-8 cell culture. Consequently, further research is necessary to understand the response of oocysts to ozone when inactivation is assessed by cell culture methods. The water industry should adopt in-vitro cell culture as a routine method for measuring the infectivity of waterborne C. parvum and C. hominis oocysts. This project has demonstrated that cell culture has equivalency with the standard CD-1 mouse assay and cell culture assays can be applied to oocysts recovered from water using approved methods. However, there needs to be a thorough, robust, and well-controlled study to compare the various cell culture-based assays for measuring C. parvum and C. hominis infectivity. This evaluation should include inter-laboratory comparisons and round-robin testing. Cell culture-based assays should also be used to assess disinfection of C. hominis isolates. Originally published by AwwaRF for its subscribers in 2004. This publication can also be purchased and downloaded via Pay Per View on Water Intelligence Online - click on the Pay Per View icon below
Comparing Cell Culture And Mouse Assays For Measuring Infectivity Of Cryptosporidium
DOWNLOAD
Author :
language : en
Publisher:
Release Date : 2004
Comparing Cell Culture And Mouse Assays For Measuring Infectivity Of Cryptosporidium written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cell culture categories.
Mouse Development
DOWNLOAD
Author : Jacek Z. Kubiak
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-08-23
Mouse Development written by Jacek Z. Kubiak and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-08-23 with Science categories.
The mouse is a perfect model organism to study mammalian, and thus indirectly also human, embryology. Most scientific achievements that have had an important impact on the understanding of basic mechanisms governing embryo development in humans, originated from mouse embryology. Stem cell research, which now offers the promise of regenerative medicine, began with the isolation and culture of mouse embryonic stem cells by Martin Evans (who received the Nobel Prize in medicine in 2007 for this achievement) and Matthew Kaufman. This book provides an overview of mouse development, spanning from oocytes before fertilization to the state-of-the-art description of embryonic and adult stem cells. The chapters, written by the leading specialists in the field, deal with the most recent discoveries in this extremely fast-developing area of research.
Animal Cells Culture And Media
DOWNLOAD
Author : D. C. Darling
language : en
Publisher: Wiley-Blackwell
Release Date : 1994
Animal Cells Culture And Media written by D. C. Darling and has been published by Wiley-Blackwell this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
This reference guide covers the fundamentals of animal cell cultures. It includes details of culture media, supplements, culture vessels, standard cell lines, passaging, cell separation techniques, cryopreservation, transfection, cell cloning and the creation of cell lines.
Mouse As A Model Organism
DOWNLOAD
Author : Cord Brakebusch
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-03-29
Mouse As A Model Organism written by Cord Brakebusch and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-03-29 with Medical categories.
Cell culture based research is important for our understanding of biological processes at the cellular and molecular level. Using this approach, the previous decades have produced a wealth of mechanistic information in all areas of biomedical research. Such in vitro research, however, lacks the complexity of in vivo investigations, where many different cell types interact with each other in a normal, three-dimensional environment, with normal levels of cytokines and growth factors. Furthermore, complex human diseases, such as cancer, diabetes or chronic inflammation, can only be modeled in vivo. Due to its small size, its short reproduction time, and the possibility to introduce specific gene mutations, the mouse has become the favourite mammalian model organism to study in vivo function of genes during development and in disease. This book combines review articles on selected subjects presented at the symposium “Mouse as a Model Organism – From Animals to Cells”, held in Rovaniemi, Finland, 2009. Among other topics, high-throughput phenotyping of mouse mutants, mouse phenotypes dependent on nature and nuture, and a spectrum of in vivo, ex vivo and in vitro methods to study cancer in mice are described. This book will give an excellent introduction to scientists interested in the use of mice as a model to understand complex biological questions in the post-genomic era. It will highlight the possibilities, but also discuss the current problems and shortcomings, to give a realistic view of the current state-of-art in this fascinating field of biomedical research.
Readings In Mammalian Cell Culture
DOWNLOAD
Author : Robert Pollack
language : en
Publisher: Cold Spring Harbor Laboratory Press
Release Date : 1981
Readings In Mammalian Cell Culture written by Robert Pollack and has been published by Cold Spring Harbor Laboratory Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981 with Nature categories.
Haematopoietic And Lymphoid Cell Culture
DOWNLOAD
Author : Margaret J. Dallman
language : en
Publisher: Cambridge University Press
Release Date : 2000-07-13
Haematopoietic And Lymphoid Cell Culture written by Margaret J. Dallman and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-07-13 with Medical categories.
Clear, affordable lab protocols for the culture of all major haematopoietic and lymphoid cell types.
Guide To Techniques In Mouse Development Part A
DOWNLOAD
Author :
language : en
Publisher: Academic Press
Release Date : 2010-08-04
Guide To Techniques In Mouse Development Part A written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-08-04 with Science categories.
This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. This volume is an update of volume 225 of MIE, "Guide to Techniques in Mouse Development", edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. During the past 17 years many new technologies or methodologies have appeared for the study of mouse development and this volume comprehensively covers these, including: new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expresion, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, rederivation of animals and use of mouse genomics.
Spontaneous And Virus Induced Transformation In Cell Culture
DOWNLOAD
Author : Jan Ponten
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Spontaneous And Virus Induced Transformation In Cell Culture written by Jan Ponten and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
A. Definitions of Transformation in vitro When normal tissues or organs are explanted to conditions favoring the growth of cells as individual units ("cell culture"), the original cell population undergoes a large variety of modifications. Only a minority of the cells will thrive and multiply and within a rather short period of time, the complex composition of the original explant is replaced by a much simplified one of only a few recogniz ably different cell types. With most organs fibroblast-like cells survive longest and outgrow other types. This is then a stable state of affairs for many gener ations. This treatise will not discuss whether this simplification and stabilization represents selection of certain pre-existing cell types or a modification of cells into only a few recognizably different categories; for an excellent review see HARRIS. (1964). Table 1. Terminology Employed to Describe Transformations in vitro Type of transformation Essential features Irregular growth Lack of contact inhibition of cell membrane movement ("ruffled membranes") between juxtaposed cells Unrestrained growth Deficient inhibition of the cell cycle (mitosis) in a crowded culture Infinite growth Capacity of cells to undergo an infinite number of di visions (formation of established cell lines) Cells may depart from this typical behavior in numerous ways involving for instance cellular morphology, immunology, chromosomes or metabolism. Such changes have, sometimes rather vaguely, been called "transformations". This is unprecise and the term "transformation" will here be used exclusively to indicate disturbances in cell growth related to neoplasia.