New Concepts For Sted Microscopy
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New Concepts For Sted Microscopy
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Author : Julia Kratz
language : en
Publisher:
Release Date : 2022
New Concepts For Sted Microscopy written by Julia Kratz and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with categories.
Fluorescence nanoscopy allows to non-invasively resolve three-dimensional cellular structures beyond the diffraction limit. One of these high resolution imaging techniques is stimulated emission depletion (STED) microscopy. However, the practically achievable resolution of a STED microscope is often limited by photobleaching. One method to overcome this limitation is tomographic STED (tomoSTED) microscopy. In tomoSTED microscopy, excited fluorophores in the sample plane are depleted by 1D STED patterns, which lead to an effective narrowing of the fluorescence-allowed area in a single direct...
Super Resolution Microscopy For Material Science
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Author : Lorenzo Albertazzi
language : en
Publisher: CRC Press
Release Date : 2024-03-26
Super Resolution Microscopy For Material Science written by Lorenzo Albertazzi and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-03-26 with Science categories.
Optical microscopy is one of the most frequently used tools in chemistry and the life sciences. However, its limited resolution hampers the use of optical imaging to many other relevant problems in different disciplines. Super-Resolution Microscopy (SRM) is a new technique that allows the resolution of objects down to a few billionth of meters (nanometers), ten times better than classical microscopes, opening up opportunities to use this tool in new fields. This book describes the theory, principles, and practice of super-resolution microscopy in the field of materials science and nanotechnology. There is a growing interest in the applications of SRM beyond biology as new synthetic materials, such as nanoscale sensors and catalysts, nanostructured materials, functional polymers, and nanoparticles, have nanoscopic features that are challenging to visualize with traditional imaging methods. SRM has the potential to be used to image and understand these cutting-edge man-made objects and guide the design of materials for novel applications. This book is an ideal guide for researchers in the fields of microscopy and materials science and chemistry as well as graduate students studying physics, materials science, biomedical engineering, and chemistry. Key Features: Contains practical guidance on Super-Resolution Microscopy (SRM), an exciting and growing tool that was awarded the Nobel Prize for chemistry in 2014 Provides a new perspective targeting materials science, unlike existing books which target readers in chemistry, life science, and biology Targets students in its core chapters, while offering more advanced material for professionals and researchers in later chapters
High Resolution Imaging In Microscopy And Ophthalmology
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Author : Josef F. Bille
language : en
Publisher: Springer
Release Date : 2019-08-13
High Resolution Imaging In Microscopy And Ophthalmology written by Josef F. Bille and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-13 with Medical categories.
This open access book provides a comprehensive overview of the application of the newest laser and microscope/ophthalmoscope technology in the field of high resolution imaging in microscopy and ophthalmology. Starting by describing High-Resolution 3D Light Microscopy with STED and RESOLFT, the book goes on to cover retinal and anterior segment imaging and image-guided treatment and also discusses the development of adaptive optics in vision science and ophthalmology. Using an interdisciplinary approach, the reader will learn about the latest developments and most up to date technology in the field and how these translate to a medical setting. High Resolution Imaging in Microscopy and Ophthalmology – New Frontiers in Biomedical Optics has been written by leading experts in the field and offers insights on engineering, biology, and medicine, thus being a valuable addition for scientists, engineers, and clinicians with technical and medical interest who would like to understand the equipment, the applications and the medical/biological background. Lastly, this book is dedicated to the memory of Dr. Gerhard Zinser, co-founder of Heidelberg Engineering GmbH, a scientist, a husband, a brother, a colleague, and a friend.
Two Approaches For A Simpler Sted Microscope Using A Dual Color Laser Or A Single Wavelength
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Author : Ancuţa Teodora Şcheul
language : en
Publisher:
Release Date : 2013
Two Approaches For A Simpler Sted Microscope Using A Dual Color Laser Or A Single Wavelength written by Ancuţa Teodora Şcheul and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
Stimulated emission depletion (STED) is a well-known super-resolution method. In a STED microscope, a doughnut-shaped beam is superimposed with the excitation beam and keeps the fluorophores in the periphery of the excitation spot in a dark state by stimulated emission, thus effectively improving the spatial resolution in a scanning configuration. This technique requires a complex setup since two laser beams, generally from different sources need to be perfectly aligned. In this work we propose two STED configurations that will simplify the setup and reduce the total cost of such a system. The basic idea in both cases is to use the same laser source for both excitation and stimulated emission depletion. In the first setup we have developed an original two-color source based on a microchip Nd-YAG laser. This microchip laser simultaneously delivers sub-ns pulses at two wavelengths, 355 nm (excitation) and 532 nm (depletion), which are generated by harmonic conversion from an Nd-YAG laser emission and offer the advantage of being intrinsically aligned and synchronized. Further work consisted in determining suitable dyes for this particular source. We have built a microscope setup based on this laser source and obtained images with an improved resolution. The confirmation of the reduction of the excitation volume is showed by Fluorescence Correlation Spectroscopy (FCS) measurements. However, the performance of this system is limited by chromatic aberrations. The combination of Selective Plane Illumination Microscopy (SPIM) with STED is considered. In the second setup the chromatic aberrations are no longer a problem since the same wavelength is used for two photon excitation and one photon depletion. By playing on the duration of the pulse (thus the instantaneous intensity), one of these two processes can be favored. Fluorescence was excited by two photon absorption with a femtosecond pulse, then depleted by one photon stimulated emission with a stretched pulse. We used the Time Correlated Single Photon Counting (TCSPC) method to study the depletion efficiency of DCM dye in solution and numerical simulations show that this method can be applied to super-resolved microscopy. In the end we present the preliminary images obtained with a home-built Two-photon Single wavelength STED microscope and the resolution improvement obtained. Further improvements are to be made to the custom microscope. In this work we have experimentally implemented, for the first time, two concepts meant to simplify the STED setups by using original sources.
Fundamentals Of Fluorescence Microscopy
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Author : Partha Pratim Mondal
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-12-12
Fundamentals Of Fluorescence Microscopy written by Partha Pratim Mondal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-12 with Science categories.
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Fluorescence Microscopy
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Author : Ulrich Kubitscheck
language : en
Publisher: John Wiley & Sons
Release Date : 2017-06-19
Fluorescence Microscopy written by Ulrich Kubitscheck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-19 with Medical categories.
While there are many publications on the topic written by experts for experts, this text is specifically designed to allow advanced students and researchers with no background in physics to comprehend novel fluorescence microscopy techniques. This second edition features new chapters and a subsequent focus on super-resolution and single-molecule microscopy as well as an expanded introduction. Each chapter is written by a renowned expert in the field, and has been thoroughly revised to reflect the developments in recent years.
Sted Microscopy Based On Axially Symmetric Polarized Vortex Beams Project Supported By The National Natural Science Foundation Of China Grant Nos 61108047 And 61475021 The Natural Science Foundation Of Beijing China Grant No 4152015 The Program For New Century Excellent Talents In Universities Of China Grant No Ncet 13 0667 And The Top Young Talents Support Program Of Beijing China Grant No Cit Tcd201404113
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Author :
language : en
Publisher:
Release Date : 2016
Sted Microscopy Based On Axially Symmetric Polarized Vortex Beams Project Supported By The National Natural Science Foundation Of China Grant Nos 61108047 And 61475021 The Natural Science Foundation Of Beijing China Grant No 4152015 The Program For New Century Excellent Talents In Universities Of China Grant No Ncet 13 0667 And The Top Young Talents Support Program Of Beijing China Grant No Cit Tcd201404113 written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.
Abstract: A stimulated emission depletion (STED) microscopy scheme using axially symmetric polarized vortex beams is proposed based on unique focusing properties of such kinds of beams. The concept of axially symmetric polarized vortex beams is first introduced, and the basic principle about the scheme is described. Simulation results for several typical beams are then shown, including radially polarized vortex beams, azimuthally polarized vortex beams, and high-order axially symmetric polarized vortex beams. The results indicate that sharper doughnut spots and thus higher resolutions can be achieved, showing more flexibility than previous schemes based on flexible modulation of both phase and polarization for incident beams.
Fundamentals Of Light Microscopy And Electronic Imaging
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Author : Douglas B. Murphy
language : en
Publisher: John Wiley & Sons
Release Date : 2012-08-22
Fundamentals Of Light Microscopy And Electronic Imaging written by Douglas B. Murphy and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-08-22 with Medical categories.
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy
Springer Handbook Of Microscopy
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Author : Peter W. Hawkes
language : en
Publisher: Springer Nature
Release Date : 2019-11-02
Springer Handbook Of Microscopy written by Peter W. Hawkes and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-11-02 with Technology & Engineering categories.
This book features reviews by leading experts on the methods and applications of modern forms of microscopy. The recent awards of Nobel Prizes awarded for super-resolution optical microscopy and cryo-electron microscopy have demonstrated the rich scientific opportunities for research in novel microscopies. Earlier Nobel Prizes for electron microscopy (the instrument itself and applications to biology), scanning probe microscopy and holography are a reminder of the central role of microscopy in modern science, from the study of nanostructures in materials science, physics and chemistry to structural biology. Separate chapters are devoted to confocal, fluorescent and related novel optical microscopies, coherent diffractive imaging, scanning probe microscopy, transmission electron microscopy in all its modes from aberration corrected and analytical to in-situ and time-resolved, low energy electron microscopy, photoelectron microscopy, cryo-electron microscopy in biology, and also ion microscopy. In addition to serving as an essential reference for researchers and teachers in the fields such as materials science, condensed matter physics, solid-state chemistry, structural biology and the molecular sciences generally, the Springer Handbook of Microscopy is a unified, coherent and pedagogically attractive text for advanced students who need an authoritative yet accessible guide to the science and practice of microscopy.
Sted Microscopy In The Visible Range
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Author : Katrin I. Willig
language : en
Publisher:
Release Date : 2006
Sted Microscopy In The Visible Range written by Katrin I. Willig and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.