Fundamentals Of Fluorescence Microscopy
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Fundamentals Of Fluorescence Microscopy
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Author : Partha Pratim Mondal
language : en
Publisher:
Release Date : 2013-12-31
Fundamentals Of Fluorescence Microscopy written by Partha Pratim Mondal and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-31 with categories.
Fundamentals Of Fluorescence Imaging
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Author : Guy Cox
language : en
Publisher: CRC Press
Release Date : 2019-04-23
Fundamentals Of Fluorescence Imaging written by Guy Cox and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-23 with Science categories.
Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism. This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.
Fundamentals Of Fluorescence Microscopy
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Author : Partha Pratim Mondal
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-12-12
Fundamentals Of Fluorescence Microscopy written by Partha Pratim Mondal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-12 with Science categories.
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Fundamentals Of Light Microscopy And Electronic Imaging
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Author : Douglas B. Murphy
language : en
Publisher: John Wiley & Sons
Release Date : 2012-11-05
Fundamentals Of Light Microscopy And Electronic Imaging written by Douglas B. Murphy and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-11-05 with Medical categories.
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy
Digital Microscopy
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Author : Greenfield Sluder
language : en
Publisher: Academic Press
Release Date : 2013-08-07
Digital Microscopy written by Greenfield Sluder and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-08-07 with Science categories.
The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. Expands coverage to include discussion of confocal microscopy not found in the previous edition Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods
Fluorescence Microscopy
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Author : B. Herman
language : en
Publisher: Taylor & Francis
Release Date : 2020-08-26
Fluorescence Microscopy written by B. Herman and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-08-26 with Science categories.
This book covers the fundamental principles of fluorescence and their application to fluorescence microscopy, and presents applications to immunofluorescence, in situ hybridization, and photomicrography. It provides troubleshooting guidance to guide the user through commonly encountered problems.
Fundamentals Of Light Microscopy
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Author : Michael Spencer
language : en
Publisher: CUP Archive
Release Date : 1982-07
Fundamentals Of Light Microscopy written by Michael Spencer and has been published by CUP Archive this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982-07 with Science categories.
Dr Spencer's account gives a concise but rigorous explanation of the principles underlying the various forms of light microscopy.
Fluorescence Microscopy In Life Sciences
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Author : Juan Carlos Stockert
language : en
Publisher: Bentham Science Publishers
Release Date : 2017-12-15
Fluorescence Microscopy In Life Sciences written by Juan Carlos Stockert and has been published by Bentham Science Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-15 with Science categories.
Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained.
Fluorescence Microscopy
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Author : Brian Herman
language : en
Publisher:
Release Date : 1998
Fluorescence Microscopy written by Brian Herman and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Fluorescence microscopy categories.
Fluoresence Microscopy is the revised, updated and expanded second edition of a best-selling introductory text on the use of fluorescence microscopy in cell biology. The book covers the fundamental principles of fluorescence and their application to fluorescence microscopy. A detailed description of the fluorescence microscope and other required equipment is given, too. Applications to immunofluorescence, in situ hybridization, and photomicrography are presented in full, practical detail. Troubleshooting guidance is provided, along with many practical hints, to guide the user through commonly encountered problems.
Introduction To Fluorescence Microscopy
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Author : J. S. Ploem
language : en
Publisher: Garland Science
Release Date : 1987
Introduction To Fluorescence Microscopy written by J. S. Ploem and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Language Arts & Disciplines categories.
Covers three main areas.The phenomenon of fluorescence, the main applications, and the most common problems.