Fundamentals Of Fluorescence Microscopy

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Fundamentals Of Fluorescence Microscopy

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Author : Partha Pratim Mondal
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-12-12

Fundamentals Of Fluorescence Microscopy written by Partha Pratim Mondal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-12 with Science categories.

This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.

Fundamentals Of Fluorescence Imaging

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Author : Guy Cox
language : en
Publisher: CRC Press
Release Date : 2019-04-23

Fundamentals Of Fluorescence Imaging written by Guy Cox and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-23 with Science categories.

Fluorescence imaging, at macro, micro, and submicro scales, has revolutionized biological science in the past 30 years. Immunolabelling has provided precise targeting of molecules in fixed tissue, while fluorescent proteins have enabled localization in living tissues. Fluorescent indicators enable imaging of dynamic changes in cell metabolism. This book covers, for the first time, imaging at all scales from macro to submicro (superresolution). Its authors include Robert Clegg, legendary teacher and researcher (who, sadly, passed away during the editing); Jim Pawley, editor of several editions of the Handbook of Biological Confocal Microscopy; the famous and now dispersed New Zealand team of Mark Cannell, Christian Soeller, and David Baddeley; Robert Hoffman, pioneer of whole-animal imaging in cancer research; Andreas Schoenle and Christian Eggeling on STED nanoscopy, and many more famous participants in this field. All the contributors are at the cutting edge of their field.

Introduction To Fluorescence

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Author : David M. Jameson
language : en
Publisher: CRC Press
Release Date : 2025-03-26

Introduction To Fluorescence written by David M. Jameson and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-03-26 with Science categories.

The phenomenon known as ‘fluorescence’ is now widely used in the chemical, physical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. This Second Edition of Introduction to Fluorescence helps readers acquire a thorough understanding of basic fluorescence theory and practice. It describes the general principles in a direct way and uses examples from a variety of disciplines to demonstrate them. Since the First Edition, significant advances in the field have appeared. For example, phasors, both lifetime and spectral phasors, have become very popular, and so a new chapter dedicated to this topic has been added in this edition. Furthermore, significant advances have been made in fluorescence microscopy methods, including super-resolution and single-molecule techniques. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects. Numerous references are given in each chapter, along with a list of particularly important references at the end of each chapter. The text incorporates more than 340 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, Chapter 13, the Appendix, provides a "Rogue’s Gallery" of the most common errors and pitfalls to avoid.

Optical Fluorescence Microscopy

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Author : Alberto Diaspro
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-11-25

Optical Fluorescence Microscopy written by Alberto Diaspro and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-25 with Science categories.

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

Fundamentals Of Light Microscopy

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Author : Michael Spencer
language : en
Publisher: CUP Archive
Release Date : 1982-07

Fundamentals Of Light Microscopy written by Michael Spencer and has been published by CUP Archive this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982-07 with Science categories.

Dr Spencer's account gives a concise but rigorous explanation of the principles underlying the various forms of light microscopy.

Fluorescence Microscopy And Fluorescent Probes

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Author : J. Slavík
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11

Fluorescence Microscopy And Fluorescent Probes written by J. Slavík and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.

Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.

Principles Of Fluorescence Spectroscopy

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Author : Joseph R. Lakowicz
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-05

Principles Of Fluorescence Spectroscopy written by Joseph R. Lakowicz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-05 with Science categories.

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

Fluorescence Microscopy In Life Sciences

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Author : Juan Carlos Stockert
language : en
Publisher:
Release Date : 2017-12-15

Fluorescence Microscopy In Life Sciences written by Juan Carlos Stockert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-15 with categories.

Fluorescence Microscopy is a precise and widely employed technique in many research and clinical areas nowadays. Fluorescence Microscopy In Life Sciences introduces readers to both the fundamentals and the applications of fluorescence microscopy in the biomedical field as well as biological research. Readers will learn about physical and chemical mechanisms giving rise to the phenomenon of luminescence and fluorescence in a comprehensive way. Also, the different processes that modulate fluorescence efficiency and fluorescence features are explored and explained. Key learning points covered in the book include: Operation of fluorescence microscopy instruments as well as the different options available today for the scientist, from the classical to the most recent approaches The wide range of biological detection possibilities that fluorescence microscopy offers in molecular biology, cell biology, histology and histopathology Fluorescent chemical compounds Breakthroughs in this field, such as non-linear microscopies and super-resolution techniques Fluorescence Microscopy In Life Sciences is intended as a detailed guide for professionals, researchers and students (including graduates Ph.D. candidates) in life sciences, with special emphasis in the biomedical field. Researchers working in allied disciplines such as pharmacology, veterinary sciences and microbiology will also benefit from the information presented in this handbook.

Flourescence Microscopy

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Author : Brian Herman
language : en
Publisher: Springer
Release Date : 1998

Flourescence Microscopy written by Brian Herman and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Medical categories.

The fluorescence microscope is an important biological laboratory tool, used to detect dells or molecules that emit fluorescence. This book deals with the fundamental principles and techniques, covering topics such as immunofluorescence, in-situ hybridization, and fluorescence photomicrography. In addition, a complete troubleshooting guide with practical hints is provided. 60 illus.

Introduction To Optical Microscopy

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Author : Jerome Mertz
language : en
Publisher: Cambridge University Press
Release Date : 2019-08

Introduction To Optical Microscopy written by Jerome Mertz and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08 with Medical categories.

Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.