Next Generation Genetically Encoded Fluorescent Sensors
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Next Generation Genetically Encoded Fluorescent Sensors
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Author : Elizabeth C. Carroll
language : en
Publisher: Frontiers Media SA
Release Date : 2021-01-19
Next Generation Genetically Encoded Fluorescent Sensors written by Elizabeth C. Carroll and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-19 with Science categories.
Awake Behaving Mesoscopic Brain Imaging
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Author : James E. Niemeyer
language : en
Publisher: Springer Nature
Release Date : 2024-10-07
Awake Behaving Mesoscopic Brain Imaging written by James E. Niemeyer and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-10-07 with Science categories.
This volume explores the latest techniques used to study how mesoscale imaging can be applied in various areas of neuroscientific research. The chapters in this book are organized into four parts: Part One highlights novel imaging modalities, such as neurotransmitter and single-cell resolution imaging and analyses of neuroimaging data in awake rodents. Part Two covers technical innovations such as head-mounted camera systems, multi-site photometry, and concurrent imaging-electrographic probes. Part Three presents methods of mesoscale imaging during normal behavior, and Part Four looks at the mesoscale imaging methods used to study neurological diseases and disorders that are critical to observe in the non-anesthetized condition. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Awake Behaving Mesoscopic Brain Imaging is a valuable resource for both novice and expert researchers who are interested in learning more about this exciting and innovative topic.
Cellular Senescence And Aging
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Author :
language : en
Publisher: Elsevier
Release Date : 2024-01-30
Cellular Senescence And Aging written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-01-30 with Science categories.
Cellular Senescence and Aging, Volume 181 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans, Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino, Methods for detection of mitochondrial reactive oxygen species in senescent cells, Assessment of cell cycle progression and mitotic slippage by videomicroscopy, The original colorimetric method to detect cellular senescence, and more.Additional sections cover Assessing microbiota composition in the context of aging, Assessing chronological aging in Saccharomyces cerevisiae, Image processing and supervised machine learning for retinal microglia characterization in senescence, Measuring telomerase activity using TRAP assays, High throughput assessment of cellular senescence, Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events, Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP), Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Updated release includes the latest information on Cellular Senescence and Aging
Imaging The Brain With Optical Methods
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Author : Anna W. Roe
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-11-11
Imaging The Brain With Optical Methods written by Anna W. Roe and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-11-11 with Medical categories.
Monitoring brain function with light in vivo has become a reality. The technology 33 of detecting and interpreting patterns of reflected light has reached a degree of 34 maturity that now permits high spatial and temporal resolution visualization at both 35 the systems and cellular levels. There now exist several optical imaging methodolo- 36 gies, based on either hemodynamic changes in nervous tissue or neurally induced 37 light scattering changes, that can be used to measure ongoing activity in the brain. 38 These include the techniques of intrinsic signal optical imaging, near-infrared optical 39 imaging, fast optical imaging based on scattered light, optical imaging with voltage 40 sensitive dyes, and two-photon imaging of hemodynamic signals. The purpose of 41 this volume is to capture some of the latest applications of these methodologies to 42 the study of cerebral cortical function. 43 This volume begins with an overview and history of optical imaging and its use 44 in the study of brain function. Several chapters are devoted to the method of intrin- 45 sic signal optical imaging, a method used to record the minute changes in optical 46 absorption due to hemodynamic changes that accompanies cortical activity. Since the 47 detected hemodynamic changes are highly localized, this method has excellent 48 spatial resolution (50–100 μm ), a resolution sufficient for visualization of fundamen- 49 tal modules of cerebral cortical function.
Natural Biomarkers For Cellular Metabolism
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Author : Vladimir V. Ghukasyan
language : en
Publisher: CRC Press
Release Date : 2014-09-26
Natural Biomarkers For Cellular Metabolism written by Vladimir V. Ghukasyan and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-09-26 with Medical categories.
From the Lab to Clinical Settings-Advances in Quantitative, Noninvasive Optical DiagnosticsNoninvasive fluorescence imaging techniques, novel fluorescent labels, and natural biomarkers are revolutionizing our knowledge of cellular processes, signaling and metabolic pathways, the underlying mechanisms for health problems, and the identification of n
Fluorescent Colors
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Author : Nora Franklin
language : en
Publisher: Publifye AS
Release Date : 2025-02-18
Fluorescent Colors written by Nora Franklin and has been published by Publifye AS this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-02-18 with Science categories.
""Fluorescent Colors"" explores the captivating realm of intensely bright colors, unraveling the science and art behind their unique glow. Delving into the phenomenon of photoluminescence, the book explains how these colors absorb light energy and re-emit it at longer wavelengths, creating their vibrant appearance. Readers will discover the crucial role of electron excitation and emission, key components of quantum mechanics, in understanding how fluorescent materials function, and explore the chemical structure of fluorescent compounds. This book bridges the gap between science and art, illustrating how these colors are utilized in diverse fields from scientific research to stunning art installations. It begins with a historical overview of fluorescence before diving into the chemistry of fluorescent dyes and pigments. By exploring the synthesis, properties, and behavior of various fluorescent molecules like organic dyes and quantum dots, the book culminates in a discussion of ethical considerations, offering a holistic perspective on this radiant subject. The book stands out by blending scientific explanations with artistic applications, making complex concepts accessible to a broad audience. Through spectroscopic data and real-world examples, ""Fluorescent Colors"" provides a comprehensive understanding, encouraging interdisciplinary thinking and highlighting the potential of fluorescent materials to solve real-world problems.
New Methods And Sensors For Membrane And Cell Volume Research
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Author :
language : en
Publisher: Academic Press
Release Date : 2021-12-01
New Methods And Sensors For Membrane And Cell Volume Research written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-12-01 with Science categories.
New Methods and Sensors for Membrane and Cell Volume Research, Volume 88 provides an overview of novel experimental approaches to study both the cell membrane and the under-membrane space – the cytosol, which have lately began drawing renewed attention. The book's overall emphasis is on fluorescent and FRET-based sensors, however, other optical (such as variants of transmission microscopy) and non-optical methods (neutron scattering and mass spectrometry) also have dedicated chapters. This volume provides a rare review of experimental approaches to study intracellular phase transitions, as well as anion channels, membrane tension and dynamics, and other topics of intense current interest. - Describes novel FRET-based membrane sensors - Reviews selected non-optical approaches to membrane structure and dynamics - Describes traditional and modern aspects of cell volume research, such as phase transitions and macromolecular crowding
Fluorescent Proteins Ii
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Author : Gregor Jung
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-01-05
Fluorescent Proteins Ii written by Gregor Jung and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-01-05 with Science categories.
Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.
The Advances And Applications Of Optogenetics
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Author : Elena G. Govorunova
language : en
Publisher: MDPI
Release Date : 2020-12-28
The Advances And Applications Of Optogenetics written by Elena G. Govorunova and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-12-28 with Science categories.
This book provides an update for the rapidly developing technology known as “optogenetics”, which is the use of genetically encoded light-sensitive molecular elements (usually derived from lower organisms) to control or report various physiological and biochemical processes within the cell. Two ongoing clinical trials use optogenetic tools for vision restoration, and optogenetic strategies have been suggested as novel therapies for several neurological, psychiatric and cardiac disorders. This Special Issue comprises two reviews and seven experimental papers on different types of light-sensitive modules widely used in optogenetic studies. These papers demonstrate the efficiency and versatility of optogenetics and are expected to be equally relevant for advanced users and beginners considering using optogenetic tools in their research.
Vectorology For Optogenetics And Chemogenetics
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Author : Mark A.G. Eldridge
language : en
Publisher: Springer Nature
Release Date : 2023-02-06
Vectorology For Optogenetics And Chemogenetics written by Mark A.G. Eldridge and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-02-06 with Medical categories.
This volume provides an overview of the viral vectors and how they are applied to chemogenetic and optogenetic tools to the study of neural circuits. The chapters in this book are organized into three parts detailing (1) viral vectors, (2) vector modifications and applications, and (3) practical advice for the delivery of viral vectors, verification of injection accuracy, and monitoring of transgene expression. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Vectorology for Optogenetics and Chemogenetics aims to be a useful practical guide to researches to help further their study in this field.