Non Radioactive Labelling

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Non Radioactive Labelling
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Author : A. J. Garman
language : en
Publisher: Elsevier
Release Date : 1997-03-20
Non Radioactive Labelling written by A. J. Garman and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-03-20 with Science categories.
Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels.This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules.Aimed at researchers without specific expertise in chemistry, the book includes: - A review of the main signal systems and labels available, indicating their strengths and weaknesses - Discussion of the most useful strategies for labelling the various biomolecules - 32 protocols covering common labelling needs - Descriptions of the factors governing protocol design, enabling protocols to be modified for different applications - Sources of information including references, data and suppliers
Nonradioactive Labeling And Detection Of Biomolecules
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Author : Christoph Kessler
language : en
Publisher: Springer
Release Date : 2012-12-06
Nonradioactive Labeling And Detection Of Biomolecules written by Christoph Kessler and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
Principles And Techniques Of Practical Biochemistry
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Author : Keith Wilson
language : en
Publisher: Cambridge University Press
Release Date : 2000-03-16
Principles And Techniques Of Practical Biochemistry written by Keith Wilson and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-03-16 with Medical categories.
New edition of biochemistry textbook which introduces principles and techniques used in undergraduate practical classes.
Non Radioactive Labelling
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Author :
language : en
Publisher:
Release Date : 1997
Non Radioactive Labelling written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
"Biological Techniques" is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels. This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.; This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules. It is aimed at researchers without specific expertise in chemistry, the book includes: a review of the main signal systems and labels available, indicating their strengths and weaknesses. It includes a discussion of the most useful strategies for labelling the various biomolecules. There are 32 protocols covering common labelling needs. There are descriptions of the factors governing protocol design, enabling protocols to be modified for different applications. It contains sources of information including references, data and suppliers.
Molecular Biology Labfax
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Author : T. A. Brown
language : en
Publisher: Elsevier
Release Date : 1998-07-28
Molecular Biology Labfax written by T. A. Brown and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-07-28 with Medical categories.
Volume 2.
Nonradioactive Analysis Of Biomolecules
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Author : Christoph Kessler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Nonradioactive Analysis Of Biomolecules written by Christoph Kessler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids - are presented in the second edition of this successful laboratory manual. Highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation, as well as PCR amplification are described in detail. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques. Thus the manual will enable scientists to plan, design and conduct the appropriate procedures which fulfill their particular requirements.
Molecular Biology And Biotechnology
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Author : John M. Walker
language : en
Publisher: Royal Society of Chemistry
Release Date : 2009
Molecular Biology And Biotechnology written by John M. Walker and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Education categories.
This popular textbook has been completely revised and updated to provide a comprehensive overview and to reflect all the latest developments in this rapidly expanding area.
From Genes To Genomes
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Author : Jeremy W. Dale
language : en
Publisher: John Wiley & Sons
Release Date : 2007-12-14
From Genes To Genomes written by Jeremy W. Dale and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-14 with Science categories.
“… an excellent book… achieves all of its goals with style, clarity and completeness… You can see the power and possibilities of molecular genetics as you read…” –Human Genetics "This volume hits an outstanding balance among readability, coverage, and detail." –Biochemistry and Molecular Biology Education Rapid advances in a collection of techniques referred to as gene technology, genetic engineering, recombinant DNA technology and gene cloning have pushed molecular biology to the forefront of the biological sciences. This new edition of a concise, well-written textbook introduces key techniques and concepts involved in cloning genes and in studying their expression and variation. The book opens with a brief review of the basic concepts of molecular biology, before moving on to describe the key molecular methods and how they fit together. This ranges from the cloning and study of individual genes to the sequencing of whole genomes, and the analysis of genome-wide information. Finally, the book moves on to consider some of the applications of these techniques, in biotechnology, medicine and agriculture, as well as in research that is causing the current explosion of knowledge across the biological sciences. From Genes to Genomes: Concepts and Applications of DNA Technology, Second Edition includes full two-colour design throughout and an accompanying website. Specific changes for the new edition include: Strengthening of gene to genome theme Updating and reinforcing of material on proteomics, gene therapy and stem cells More eukaryotic/mammalian examples and less focus on bacteria This textbook is must-have for all undergraduates studying intermediate molecular genetics within the biological and biomedical sciences. It is also of interest for researchers and all those needing to update their knowledge of this rapidly moving field.
Analytical Molecular Biology
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Author : Ginny C. Saunders
language : en
Publisher: Royal Society of Chemistry
Release Date : 1999
Analytical Molecular Biology written by Ginny C. Saunders and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Science categories.
DNA technology has revolutionized molecular biology, with far-reaching implications in health care, pharmaceuticals, agriculture, and forensics. This book, produced under the VAM (Valid Analytical Measurement) initiative, addresses the applicability, reliability and validation of the many methods employed. Since analytical molecular biology has typically developed in the academic and medical research environments, guidelines are also provided to ease the transition of cutting-edge techniques from the research environment to the laboratory. 27 illus.
Gene Cloning And Dna Analysis
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Author : T. A. Brown
language : en
Publisher: John Wiley & Sons
Release Date : 2016-01-19
Gene Cloning And Dna Analysis written by T. A. Brown and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-01-19 with Mathematics categories.
GENE CLONING & DNA ANALYSIS.