Nonradioactive Labeling And Detection Of Biomolecules
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Nonradioactive Labeling And Detection Of Biomolecules
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Author : Christoph Kessler
language : en
Publisher: Springer
Release Date : 2012-12-06
Nonradioactive Labeling And Detection Of Biomolecules written by Christoph Kessler and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
Nonradioactive Analysis Of Biomolecules
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Author : Christoph Kessler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Nonradioactive Analysis Of Biomolecules written by Christoph Kessler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
New techniques and updated protocols for the detection and analysis of biomolecules - proteins, glycoproteins and nucleic acids - are presented in the second edition of this successful laboratory manual. Highly sensitive systems which are widely used in molecular biological and biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody/antigen binding or hybridisation, as well as PCR amplification are described in detail. The clearly structured step-by-step protocols with practical hints and a troubleshooting guide are complemented by chapters on the theoretical background and the application of the techniques. Thus the manual will enable scientists to plan, design and conduct the appropriate procedures which fulfill their particular requirements.
Nonisotopic Probing Blotting And Sequencing
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Author : Larry J. Kricka
language : en
Publisher: Elsevier
Release Date : 1995-06-01
Nonisotopic Probing Blotting And Sequencing written by Larry J. Kricka and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-06-01 with Science categories.
Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols. - Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules - Gives protocols for nonisotopic DNA sequencing - new in this edition - Gives extensive, practical information - Presents background information for each method - Provides expert accounts from the inventor or developer of each method - Contains seven entirely new chapters - Covers all major types of nonisotopic procedures for labeling and detection
Nonradioactive Analysis Of Biomolecules
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Author : Christoph Kessler
language : en
Publisher: Springer Science & Business Media
Release Date : 2000-03-11
Nonradioactive Analysis Of Biomolecules written by Christoph Kessler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-03-11 with Science categories.
Highly sensitive systems which are widely used in molecular biological & biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody-antigen binding or hybridisation, as well as PCR amplification are described in detail.
Molecular Biology
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Author : Susan J. Karcher
language : en
Publisher: Academic Press
Release Date : 1995-11-28
Molecular Biology written by Susan J. Karcher and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-11-28 with Science categories.
This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. - Includes a glossary of over 200 terms important for understanding molecular biology - Uses an inexpensive source of eukaryotic cells - great for schools on a budget - Includes Methods Locator that provides instant access to the latest methods - Contain clearly written, easy-to-follow, student-tested instructions: - Sterile techniques - Phage titration - Gel electrophoresis of DNA - Restriction enzyme digestion - Plasmid isolation - Transformation of E. Coli - Recombinant DNA cloning - Nick translation labeling - Nonradioactive primer labelling - Nonradioactive DNA detection - Southern blotting - Colony hybridization - Purification of plant DNA - RNA purification - Northern blotting - Purification of poly A+ RNA - Polymerase chain reaction (PCR)
Nonradioactive Labeling And Detection Of Biomolecules
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Author : Christoph Kessler
language : en
Publisher: Springer Verlag
Release Date : 1992-01-01
Nonradioactive Labeling And Detection Of Biomolecules written by Christoph Kessler and has been published by Springer Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992-01-01 with Science categories.
Non Radioactive Labelling
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Author : A. J. Garman
language : en
Publisher: Elsevier
Release Date : 1997-03-20
Non Radioactive Labelling written by A. J. Garman and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-03-20 with Science categories.
Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels.This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules.Aimed at researchers without specific expertise in chemistry, the book includes: - A review of the main signal systems and labels available, indicating their strengths and weaknesses - Discussion of the most useful strategies for labelling the various biomolecules - 32 protocols covering common labelling needs - Descriptions of the factors governing protocol design, enabling protocols to be modified for different applications - Sources of information including references, data and suppliers
Basic Techniques In Molecular Biology
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Author : Stefan Surzycki
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Basic Techniques In Molecular Biology written by Stefan Surzycki and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Gene Transfer To Plants
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Author : Ingo Potrykus
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
Gene Transfer To Plants written by Ingo Potrykus and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.
Fluorescence Microscopy And Fluorescent Probes
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Author : J. Slavík
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Fluorescence Microscopy And Fluorescent Probes written by J. Slavík and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
Fluorescence microscopy images can be easily integrated into current video and computer image processing systems. People like visual observation; they like to watch a television or computer screen, and fluorescence techniques are thus becoming more and more popular. Since true in vivo experiments are simple to perform, samples can be directly seen and there is always the possibility of manipulating the samples during the experiments; it is an ideal technique for biology and medicine. Images are obtained by a classical (now called wide-field) fluorescence microscope, a confocal scanning microscope, upright or inverted, with epifluorescence or transmission. Computerized image processing may improve definition, and remove glare and scattered light signal. It also makes it possible to compute ratio images (ratio imaging both in excitation and in emission) or lifetime imaging. Image analysis programs may supply a great deal of additional data of various types, starting with calculations of the number of fluorescent objects, their shapes, brightness, etc. Fluorescence microscopy data may be complemented by classical measurement in the cuvette yr by flow cytometry.