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Numerical Computer Methods Part E


Numerical Computer Methods Part E
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Numerical Computer Methods Part E


Numerical Computer Methods Part E
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Author :
language : en
Publisher: Elsevier
Release Date : 2004-06-02

Numerical Computer Methods Part E written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-06-02 with Science categories.


The contributions in this volume emphasize analysis of experimental data and analytical biochemistry, with examples taken from biochemistry. They serve to inform biomedical researchers of the modern data analysis methods that have developed concomitantly with computer hardware.Selected Contents: A practical approach to interpretation of SVD results; modeling of oscillations in endocrine networks with feedback; quantifying asynchronous breathing; sample entropy; wavelet modeling and processing of nasal airflow traces



Numerical Computer Methods Part D


Numerical Computer Methods Part D
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Author :
language : en
Publisher: Elsevier
Release Date : 2004-05-25

Numerical Computer Methods Part D written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-05-25 with Science categories.


The aim of Numerical Computer Methods, Part D is to brief researchers of the importance of data analysis in enzymology, and of the modern methods that have developed concomitantly with computer hardware. It is also to validate researchers' computer programs with real and synthetic data to ascertain that the results produced are what they expected. - Selected Contents: - Prediction of protein structure - Modeling and studying proteins with molecular dynamics - Statistical error in isothermal titration calorimetry - Analysis of circular dichroism data - Model comparison methods



Embryonic Stem Cells


Embryonic Stem Cells
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Author :
language : en
Publisher: Elsevier
Release Date : 2006-12-08

Embryonic Stem Cells written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-12-08 with Medical categories.


This is the first of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.* Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering * Presents the latest most innovative technologies * Addresses therapeutic relevance including FDA compliance and tissue engineering



Functional Glycomics


Functional Glycomics
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Author : Minoru Fukuda
language : en
Publisher: Elsevier
Release Date : 2006-12-08

Functional Glycomics written by Minoru Fukuda and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-12-08 with Science categories.


In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the second of three in the Methods in Enzymology series, including Glycobiology (vol. 415) and Glycomics (vol. 416), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are designed to provide an introduction of new methods to a large variety of readers who would like to participate in and contribute to the advancement of glycobiology. The methods covered include structural analysis of carbohydrates, biological and chemical synthesis of carbohydrates, expression and determination of ligands for carbohydrate-binding proteins, gene expression profiling including micro array, and generation of gene knockout mice and their phenotype analyses.



Stem Cell Tools And Other Experimental Protocols


Stem Cell Tools And Other Experimental Protocols
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Author :
language : en
Publisher: Elsevier
Release Date : 2006-12-12

Stem Cell Tools And Other Experimental Protocols written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-12-12 with Technology & Engineering categories.


This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. - Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering - Presents the latest most innovative technologies - Addresses therapeutic relevance including FDA compliance and tissue engineering



Measuring Biological Responses With Automated Microscopy


Measuring Biological Responses With Automated Microscopy
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Author :
language : en
Publisher: Elsevier
Release Date : 2006-11-02

Measuring Biological Responses With Automated Microscopy written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-11-02 with Science categories.


The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.



Osmosensing And Osmosignaling


Osmosensing And Osmosignaling
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Author :
language : en
Publisher: Elsevier
Release Date : 2007-10-01

Osmosensing And Osmosignaling written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-01 with Science categories.


For over fifty years the Methods in Enzymology series has been the critically acclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume features articles on the topic of osmosensing and osmosignaling written by experts in the field.



Rna Modification


Rna Modification
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Author :
language : en
Publisher: Elsevier
Release Date : 2007-09-13

Rna Modification written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-13 with Science categories.


The presence of modified nucleotides in cellular RNAs has been known for decades and over 100 distinct RNA modifications have been characterized to date. While the exact role of many of these modifications is still unclear, many are highly conserved across evolution and most contribute to the overall fitness of the organism. In recent years, new methods and bioinformatics approaches have been developed for the dissection of modification pathways and functions. These methods intersect a number of related fields, ranging from RNA processing to comparative genomics and systems biology. In addition, many of the techniques described in this volume have broad applicability, particularly in regards to the isolation, characterization, and reconstitution of ribonucleoprotein complexes, expanding the experimental repertoire available to all RNA researchers.



Lipidomics And Bioactive Lipids Mass Spectrometry Based Lipid Analysis


Lipidomics And Bioactive Lipids Mass Spectrometry Based Lipid Analysis
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Author :
language : en
Publisher: Elsevier
Release Date : 2007-11-26

Lipidomics And Bioactive Lipids Mass Spectrometry Based Lipid Analysis written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-26 with Science categories.


This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Qualitative Analysis and Quantitative Assessment of Changes in Neutral Glycerol Lipid Molecular Species within Cells; Glycerophospholipid identification and quantitation by electrospray ionization mass spectrometry; Detection and Quantitation of Eicosanoids via High Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry; Structure-specific, quantitative methods for "lipidomic" analysis of sphingolipids by tandem mass spectrometry; Analysis of Ubiquinones, Dolichols and Dolichol Diphosphate-Oligosaccharides by Liquid Chromatography Electrospray Ionization Mass Spectrometry; Extraction and Analysis of Sterols in Biological Matrices by High-Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry; The Lipid Maps Initiative in Lipidomics; Basic analytical systems for lipidomics by mass spectrometry in Japan; The European Lipidomics Initiative Enabling technologies; Lipidomic analysis of Signaling Pathways; Bioinformatics for Lipidomics; Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins and Protectins; A guide to biochemical systems modeling of sphingolipids for the biochemist; and Quantitation and Standardization of Lipid Internal Standards for Mass Spectroscopy.



Lipidomics And Bioactive Lipids Lipids And Cell Signaling


Lipidomics And Bioactive Lipids Lipids And Cell Signaling
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Author :
language : en
Publisher: Elsevier
Release Date : 2007-11-12

Lipidomics And Bioactive Lipids Lipids And Cell Signaling written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-12 with Science categories.


This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Phospholipase A1 assays using a radio-labeled substrate and mass spectrometry; Real-time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids; Analysis and Pharmacological Targeting of Phospholipase C â interactions with G proteins; Biochemical Analysis of Phospholipase D.; Measurement of Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor; Quantitative measurement of PtdIns(3,4,5)P3; Measuring Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated Phosphoproteins In Primary Lymphocytes; Regulation of Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner proteins; Biochemical Analysis of Inositol Phosphate Kinases; Analysis of the phosphoinositides and their aqueous metabolites; Combination of C17-sphingoid base homologues and mass spectrometry analysis as a new approach to study sphingolipid metabolism; Measurement of mammalian sphingosine-1-phosphate phosphohydrolase activity in vitro and in vivo; A rapid and sensitive method to measure secretion of sphingosine-1-phosphate; Ceramide Kinase and Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases from Saccharomyces cerevisiae.