Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 248 Xenopus Eleutheroembryonic Thyroid Assay Xeta

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Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 248 Xenopus Eleutheroembryonic Thyroid Assay Xeta

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Author : OECD
language : en
Publisher: OECD Publishing
Release Date : 2019-06-18

Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 248 Xenopus Eleutheroembryonic Thyroid Assay Xeta written by OECD and has been published by OECD Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-18 with categories.

The purpose of the test is to measure the capacity of a chemical to activate or inhibit the transcription of a genetic construct (THb/ZIP-GFP X. laevis eleutheroembryos) either by binding to the thyroid hormone receptor or by modifying the amount of thyroid hormone available for transcription. The test is based on the use of a transgenic frog (possessing the genetic construction THb/ZIP-GFP) of the species X. laevis. GFP is a fluorescent protein that can be used to affirm the presence of the substance and to follow its action. This test is based on the quantification of fluorescence, and thus of the GFP protein, throughout the eleutheroembryo exposed to a test chemical. Indeed, if the fluorescence is > 12% by including the highest concentration of the test, the test is considered positive. Organisms are exposed in chemically inert cell culture plates (6 wells). Each well contains ten organisms in solution. In a run, 20 organisms are exposed to each test concentration. Tadpoles are exposed to the test chemical at NF stage 45 for a duration of 72 hours. A positive result of the XETA test indicates in vivo that a substance induces damage to the receptor and metabolization of the thyroid hormone. A negative result indicates that, under test conditions, the test substance does not induce detectable damage to the thyroid and its functioning.

Lignes Directrices De L Ocde Pour Les Essais De Produits Chimiques Section 2 Essai No 248 Essai Thyro Dien Sur Leuth Ro Embryons De X Nope Xeta

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Author : OECD
language : fr
Publisher: OECD Publishing
Release Date : 2019-06-18

Lignes Directrices De L Ocde Pour Les Essais De Produits Chimiques Section 2 Essai No 248 Essai Thyro Dien Sur Leuth Ro Embryons De X Nope Xeta written by OECD and has been published by OECD Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-18 with categories.

Le but de l’essai est de mesurer la capacité d’un produit chimique pour activer ou inhiber la transcription d’une construction génétique (THb/ZIP-GFP X. laevis eleutheroembryos) soit par liaison au récepteur de l’hormone thyroïdienne soit en modifiant la quantité de l’hormone thyroïdienne disponible pour la transcription. L’essai est basé sur l’utilisation d’une grenouille transgénique (possédant la construction génétique THb/ZIP-GFP) de l’espèce X. laevis. La GFP est une protéine fluorescente qui permet d’affirmer la présence de la substance et de suivre son action. Ce test repose sur la quantification de la fluorescence, et donc de la protéine GFP, dans l’ensemble de l’eleutheroembryon exposé au produit chimique testé. En effet, si la flurorescence est > 12% en incluant la plus forte concentration du test, le test est considéré comme positif. Les organismes sont exposés dans des plaques de culture cellulaire chimiquement inertes (6 puits). Chaque puits doit contenir dix organismes en solution. Dans une épreuve, 20 organismes sont exposés à chaque concentration d’essai. Les grenouilles sont exposées aux produits chimiques au stade NF45. La durée d’exposition est de 72H. Un résultat positif de l'essai XETA indique in vivo qu'une substance induit des dommages sur le récepteur et la métabolisation de l’hormone thyroïdienne. Un résultat négatif indique que, dans les conditions d'essai, le produit chimiques testé n'induit pas des dommages discernables sur la thyroïde et son fonctionnement.

Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 231 Amphibian Metamorphosis Assay

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Author : OECD
language : en
Publisher: OECD Publishing
Release Date : 2009-09-08

Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 231 Amphibian Metamorphosis Assay written by OECD and has been published by OECD Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-09-08 with categories.

This Test Guideline describes an amphibian metamorphosis assay intended to screen substances which may interfere with the normal functioning of the hypothalamo-pituitary-thyroid axis. The assay was validated with the species Xenopus laevis, which is ...

Clinical Safety And Efficacy Testing Of Cosmetics

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Author : William Waggoner
language : en
Publisher: CRC Press
Release Date : 1989-11-27

Clinical Safety And Efficacy Testing Of Cosmetics written by William Waggoner and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-11-27 with Medical categories.

Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 241 The Larval Amphibian Growth And Development Assay Lagda

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Author : OECD
language : en
Publisher: OECD Publishing
Release Date : 2015-07-28

Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 241 The Larval Amphibian Growth And Development Assay Lagda written by OECD and has been published by OECD Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-07-28 with categories.

This Test Guideline describes the Medaka Extended One Generation Test (MEOGRT), which exposes fish over multiple generations to give data relevant to ecological hazard and risk assessment of chemicals, including suspected endocrine disrupting chemicals (EDCs). Exposure in the MEOGRT starts with ...

Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 251 Rapid Androgen Disruption Activity Reporter Radar Assay

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Author : OECD
language : en
Publisher: OECD Publishing
Release Date : 2022-06-30

Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 251 Rapid Androgen Disruption Activity Reporter Radar Assay written by OECD and has been published by OECD Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-06-30 with categories.

The Rapid Androgen Disruption Activity Reporter (RADAR) Test Guideline describes an aquatic assay that utilizes transgenic Oryzias latipes (O. latipes, Japanese medaka) eleutheroembryos at day post hatch zero, in a multi-well format to detect chemicals active on the androgen axis.

Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 234 Fish Sexual Development Test

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Author : OECD
language : en
Publisher: OECD Publishing
Release Date : 2011-07-28

Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 234 Fish Sexual Development Test written by OECD and has been published by OECD Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-07-28 with categories.

This Test Guideline describes an assay that assesses early life-stage effects and potential adverse consequences of putative endocrine disrupting chemicals (e.g. oestrogens, androgens and steroidogenesis inhibitors) on fish sexual development. In ...

Oecd Guidelines For The Testing Of Chemicals Oecd Series On Testing And Assessment Detailed Review Paper On Amphibian Metamorphosis Assay For The Detection Of Thyroid Active Substances

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Author : OECD
language : en
Publisher: OECD Publishing
Release Date : 2004-11-01

Oecd Guidelines For The Testing Of Chemicals Oecd Series On Testing And Assessment Detailed Review Paper On Amphibian Metamorphosis Assay For The Detection Of Thyroid Active Substances written by OECD and has been published by OECD Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-11-01 with categories.

This Detailed Review Paper (DRP) provides the current state-of-the-knowledge in the area of amphibian metamorphosis with the view to use amphibian metamorphosis as a model for the detection of chemicals affecting the thyroid axis in vertebrates.

Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 210 Fish Early Life Stage Toxicity Test

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Author : OECD
language : en
Publisher: OECD Publishing
Release Date : 2025-06-25

Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 210 Fish Early Life Stage Toxicity Test written by OECD and has been published by OECD Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-06-25 with categories.

The test method described in this Test Guideline, is intended to define the lethal and sub-lethal effects of chemicals on the early life stages of the species tested. The early-life stages of fish are exposed to five concentrations of the test ...