Pcr Guru
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Pcr Guru
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Author : Ayaz Najafov
language : en
Publisher: Academic Press
Release Date : 2016-11-28
Pcr Guru written by Ayaz Najafov and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-11-28 with Medical categories.
PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic science underlying it. It then discusses PCR at the bench level, starting with detailed description and tips on primer design, and continuing with the standard protocols used to perform PCR. Provides troubleshooting tips for various types of modifications of standard protocols Contains unique “Good Practices and Tips that are indispensable for the beginner and expert alike Features “Special Cases with applications of PCR, optimization, and troubleshooting Includes detailed appendices with tables, figures, and key protocols Organized as a systematic, concentrated resource to save time when addressing a PCR problem
Pcr Topics
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Author : Arndt Rolfs
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-12-01
Pcr Topics written by Arndt Rolfs and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-01 with Medical categories.
PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.
Pcr
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Author : Lucília Domingues
language : en
Publisher: Springer Nature
Release Date : 2023-09-23
Pcr written by Lucília Domingues and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-09-23 with Science categories.
This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Principles And Technical Aspects Of Pcr Amplification
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Author : Elizabeth van Pelt-Verkuil
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-03-14
Principles And Technical Aspects Of Pcr Amplification written by Elizabeth van Pelt-Verkuil and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-14 with Science categories.
Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).
Pcr Protocols
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Author : John M. S. Bartlett
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Pcr Protocols written by John M. S. Bartlett and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Pcr
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Author : MCPHERSON.
language : en
Publisher: Garland Science
Release Date : 2000
Pcr written by MCPHERSON. and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Juvenile Nonfiction categories.
A thoroughly updated version of the successful first edition, with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections.
Real Time Pcr
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Author : M Tevfik Dorak
language : en
Publisher: Garland Science
Release Date : 2007-02-08
Real Time Pcr written by M Tevfik Dorak and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-02-08 with Science categories.
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
Pcr
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Author : Mike McPherson
language : en
Publisher: Garland Science
Release Date : 2007-01-25
Pcr written by Mike McPherson and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-01-25 with Science categories.
A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.
The Pcr Revolution
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Author : Stephen A. Bustin
language : en
Publisher: Cambridge University Press
Release Date : 2010
The Pcr Revolution written by Stephen A. Bustin and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Science categories.
Examines the latest innovations and the overall impact of PCR on areas of molecular research.
Pcr
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Author : Clive R. Newton
language : en
Publisher: Springer
Release Date : 1997-01-01
Pcr written by Clive R. Newton and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-01-01 with Cytodiagnosis categories.