Peptide Research Protocols
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Peptide Research Protocols
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Author : Janet J. Maguire
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Peptide Research Protocols written by Janet J. Maguire and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Medical categories.
A panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of the endothelin system from its synthesis and metabolism, to its function in health and disease. Theses methods use state-of-the-art molecular techniques to quantify the expression of mRNA for both endothelin receptors and the endothelin converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function.
Peptide Protocols
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Author : MD William A. Seeds
language : en
Publisher:
Release Date : 2020-08-24
Peptide Protocols written by MD William A. Seeds and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-08-24 with Medical categories.
Peptide Characterization And Application Protocols
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Author : Gregg B. Fields
language : en
Publisher: Humana Press
Release Date : 2010-11-19
Peptide Characterization And Application Protocols written by Gregg B. Fields and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-19 with Science categories.
This book is dedicated to the characterization of peptides and their applications for the study of biochemical systems. The contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques. Part II, Application, presents a variety of specific applications for synthetic peptides. This book is an indispensable aid in the pursuit of new directions in peptide research.
Computational Peptide Science
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Author : Thomas Simonson
language : en
Publisher: Humana
Release Date : 2022-03-18
Computational Peptide Science written by Thomas Simonson and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-03-18 with Science categories.
This volume details current and new computational methodologies to study peptides. Chapters guide readers through antimicrobial peptides, foldability, amyloid sheet formation, membrane-active peptides, organized peptide assemblies, protein-peptide interfaces, prediction of peptide-MHC complexes, advanced free energy simulations for peptide binding, and methods for high throughput peptide or miniprotein design. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials, software, and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Computational Peptides Science: Methods and Protocols aims to provide concepts, methods, and guidelines to help both novices and experienced workers benefit from today's new opportunities and challenges.
Cell Penetrating Peptides
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Author : Ülo Langel
language : en
Publisher: Humana Press
Release Date : 2011-08-24
Cell Penetrating Peptides written by Ülo Langel and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-24 with Science categories.
In recent years, a new understanding of cell-penetrating peptides has emerged, helping researchers to expand beyond a number of long-held dogmas. In Cell-Penetrating Peptides: Methods and Protocols, expert researchers explore the latest information on cell-penetrating peptides (CPPs), providing insight into the most important and contemporary areas of CPP research. Chapters address the historical background of CPP studies, provide an overview of the growing field of research, investigate methods for testing CPP mechanisms, present a summary of methods that attempt to use properties of CPPs to study biochemical intracellular mechanisms of interaction and signal transduction, and include new ideas for turning CPP-based strategies into drugs. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and current, Cell-Penetrating Peptides: Methods and Protocols is an essential guide that allows researchers to study intracellular mechanisms in new ways, and to promote the future development of novel drugs.
Peptide Nucleic Acids
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Author : Peter E. Nielsen
language : en
Publisher: Garland Science
Release Date : 2004
Peptide Nucleic Acids written by Peter E. Nielsen and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with DNA categories.
Peptide Nucleic Acids, Second Edition has been extensively revised, updated, and enlarged to contain many new chapters covering the most recent topics and applications in this fast-moving field. The book contains state-of-the-art protocols and applications on all aspects of peptide nucleic acids. Concepts are clearly explained with each chapter containing concise background information. Written by leading experts in the field, the book is an invaluable and complete reference work on this novel and exciting area.
Peptide Analysis Protocols
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Author : Ben M. Dunn
language : en
Publisher: Humana
Release Date : 2013-12-06
Peptide Analysis Protocols written by Ben M. Dunn and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-06 with Science categories.
As the technology base for the preparation of increasingly c- plex peptides has improved, the methods for their purification and ana- sis have also been improved and supplemented. Peptide science routinely utilizes tools and techniques that are common to organic chemistry, p- tein chemistry, biophysical chemistry, enzymology, pharmacology, and molecular biology. A fundamental understanding of each of these areas is essential for interpreting all of the data that a peptide scientist may see. The purpose of Peptide Analysis Protocols is to provide the novice with sufficient practical information necessary to begin developing useful analysis and separation skills. Understanding and developing these skills will ultimately yield a scientist with broadened knowledge and good problem-solving abilities. Although numerous books that address d- ferent specialties, such as HPLC, FAB-MS, CE, and NMR, have been written, until now no single volume has reviewed all of these techniques with a focus on "getting started" in separation and analysis of peptides. This volume will also provide those who already possess practical knowledge of the more advanced aspects of peptide science with detailed applications for each of these protocols. Because the chapters have been written by researchers active in each of the fields that they discuss, a great deal of information on and insight into solution of real problems that they have encountered is presented. Examplary results are clearly demonstrated and discussed. For more advanced investi- tions, supplementary experiments are often suggested.
Peptide Microarrays
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Author : Marina Cretich
language : en
Publisher: Humana Press
Release Date : 2009-10-20
Peptide Microarrays written by Marina Cretich and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-10-20 with Science categories.
Due to their versatility, along with the diminishing costs of library synthesis and the growth of commercial support, peptide microarrays will likely expand beyond being just a research tool into an adaptable and powerful platform to be harnessed for wider drug discovery and point-of-care applications. In Peptide Microarrays: Methods and Protocols, experts in the field provide a cutting-edge view of peptide array technology, its applications, and technical issues. After examining how peptides can characterize proteins and clarify, at the amino acid level, the molecular recognition events in which they are involved, the volume goes on to cover topics such as the production and use of peptide arrays for enzyme and binding motifs characterization, epitope mapping, and diagnostics, the newest technological advancements, as well as software and web tools for the design of peptide arrays and for the analysis of output data. As a volume in the highly successful Methods in Molecular BiologyTM series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Peptide Microarrays: Methods and Protocols promises to serve scientists with its unique insights and novel solutions in peptide array technology in order to advance this vital field.
Peptide Synthesis
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Author : Waleed M. Hussein
language : en
Publisher: Humana
Release Date : 2019-12-27
Peptide Synthesis written by Waleed M. Hussein and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-12-27 with Science categories.
This book provides a variety of procedures for synthetically producing peptides and their derivatives, ensuring the kind of precision that is of paramount importance for successful synthesis. Numerous techniques relevant to drugs and vaccines are explored, such as conjugation and condensation methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Peptide Synthesis: Methods and Protocols serves as an essential guide to the many crucial processes that will allow researchers to efficiently prepare, purify, characterize, and use peptides for chemical, biochemical, and biological studies.
Peptide Based Drug Design
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Author : Laszlo Otvos
language : en
Publisher: Humana Press
Release Date : 2008-11-01
Peptide Based Drug Design written by Laszlo Otvos and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-01 with Medical categories.
Due to their high specificity and low toxicity profile, peptides have once again become central to the development of new drugs. In Peptide-Based Drug Design: Methods and Protocols, expert researchers provide a handbook which offers a selection of research and production tools suitable for transforming a promising protein fragment or stand-alone native peptide into a pharmaceutically acceptable composition. The volume delves into contemporary, cutting-edge subjects such as hit isolation and target validation, computer-aided design, sequence modifications to satisfy pharmacologists, in vivo stability and imaging, and the actual production of difficult sequences. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include readily reproducible, step-by-step laboratory protocols, lists of materials, and the Notes section, which highlights tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Peptide-Based Drug Design: Methods and Protocols shows its subject to be an independent science on the rise, and provides scientists with a clear, concise guide for continuing this vital research.