Plant Microtechnique And Microscopy
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Plant Microtechnique And Microscopy
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Author : Steven E. Ruzin
language : en
Publisher: Oxford University Press, USA
Release Date : 1999
Plant Microtechnique And Microscopy written by Steven E. Ruzin and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Science categories.
Plant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the context of the whole plant. Plant Microtechnique and Microscopy offers uniquely in-depth coverage of this reinvigorated field. Thoroughly covering classical aspects of microscope slide preparation, it goes a step beyond all other available manuals by also documenting the theory and practice of modern applications. The text opens with single-page "Quick Start" protocols that provide students with fundamental instructions to complete eight of the most common microtechnique protocols used today. The following sections cover the theory and practice of microtechnique. The traditional paraffin method is demonstrated by explicit step-by-step protocols, and theoretical background is incorporated to give students the tools required to design their own experiments and to interpret existing results. In addition, modern applications such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in situ hybridization are discussed in detail. The manual also contains a definitive chapter on microscopy and describes, in both text and diagrams, the optical principles of techniques such as phase contrast and DIC as well as confocal and deconvolution wide-field microscopy. Appendices on laboratory practice (chemical toxicities, common calculations, and buffer tables), an extensive appendix on optics and its application to the microscope, and an extensive bibliography of over 550 references are also included. Ideal for courses in plant biology, Plant Microtechnique and Microscopy also serves as an indispensable reference for all students of microscopy, histology, and histological technique. It is a valuable addition to every biological laboratory.
Plant Microtechnique
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Author : Donald Alexander Johansen
language : en
Publisher:
Release Date : 1940
Plant Microtechnique written by Donald Alexander Johansen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1940 with Botany categories.
Methods In Plant Microtechnique
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Author : N. Prakash
language : en
Publisher:
Release Date : 1986
Methods In Plant Microtechnique written by N. Prakash and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with Botanical microscopy categories.
Plant Microtechniques And Protocols
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Author : Edward Chee Tak Yeung
language : en
Publisher: Springer
Release Date : 2015-09-19
Plant Microtechniques And Protocols written by Edward Chee Tak Yeung and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-09-19 with Science categories.
A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.
Methods In Plant Microtechnique
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Author : N. Prakash
language : en
Publisher:
Release Date : 1980
Methods In Plant Microtechnique written by N. Prakash and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1980 with Microscopy categories.
Botanical Microtechnique And Cytochemistry
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Author : Graeme P. Berlyn
language : en
Publisher: Iowa State Press
Release Date : 1976
Botanical Microtechnique And Cytochemistry written by Graeme P. Berlyn and has been published by Iowa State Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1976 with Science categories.
Plant Microtechniques And Protocols
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Author : Edward Chee Tak Yeung
language : en
Publisher:
Release Date : 2015
Plant Microtechniques And Protocols written by Edward Chee Tak Yeung and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach. The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various structural methods for the first time. This book analyzes a range of topics in order to generate cross-talks among scientists in different research disciplines. The first section of this volume covers the more commonly used embedding methods, with emphasis on the preparative methods for light and electron microscopy. A number of cell biology related protocols are compiled in the second section to showcase the usefulness of various techniques based on different processing and staining methods. The third section highlights some common and recent procedures in wood preparation. The last section includes botanical methods related to archaeological uses of plant materials. A special chapter on field and herbarium procedures is also included to serve as a guide to students interested in plant collection and taxonomic studies.
Botanical Microtechnique And Cytochemistry
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Author : Graeme P. Berlyn
language : en
Publisher:
Release Date : 1997-01-01
Botanical Microtechnique And Cytochemistry written by Graeme P. Berlyn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-01-01 with categories.
1. Microscope construction, Use, and care; 2. Collecting and Subdividing plants materials 3. Fixation and storage 4. Processing and embedding 5. Microtomy 6. Staining Paraffin and plastic sections 7. Preparation of whole mounts and macerations 8. Principles of optics and microscopy 9 Types of microscopy 10. Photomicrography; 11. Cytochemistry
Manual Of Advanced Plant Microtechnique
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Author : Frank Denmire Venning
language : en
Publisher:
Release Date : 1954
Manual Of Advanced Plant Microtechnique written by Frank Denmire Venning and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1954 with Botanical microscopy categories.
General laboratory procedures and techniques. Special Techniques for special structures. Cytological methods.
Botanical Microtechnique
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Author : Albrecht Zimmermann
language : en
Publisher:
Release Date : 1893
Botanical Microtechnique written by Albrecht Zimmermann and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1893 with Botanical chemistry categories.