Plant Nitric Oxide Methods And Protocols
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Plant Nitric Oxide Methods And Protocols
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Author : Kapuganti Jagadis Gupta
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-05-05
Plant Nitric Oxide Methods And Protocols written by Kapuganti Jagadis Gupta and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-05 with Science categories.
With numerous methods available for the quantification of nitric oxide (NO), this detailed book explores their advantages and disadvantages in order to provide a foundation for further research in plant nitric oxide. After providing a useful practical guide to choosing a technique for measuring NO from plant materials, the book continues with chapters on chemiluminescence, diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well as many other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory methods, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Nitric Oxide: Methods and Protocols serves as a valuable guide to all researchers working and intending to work in the field of plant nitric oxide research.
Nitric Oxide
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Author : Alexander Mengel
language : en
Publisher: Humana
Release Date : 2019-04-13
Nitric Oxide written by Alexander Mengel and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-13 with Science categories.
This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part one looks at NO treatments using gaseous nitric oxide and detection using a NO-sensitive electrode, electron spin resonance, and fluorescence-based NO-sensor proteins. Part two talks about various techniques used to detect and identify NO-dependent modifications, such as biotin-switch assay and quantification of s-nitrosated proteins. Part three focuses on the study of s-nitrosothiol homeostasis and denitrosation activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and authoritative, Nitric Oxide: Methods and Protocols is a valuable resource for anyone interested in learning more about this evolving field.
Nitrogen Metabolism In Plants
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Author : Jagadis Gupta Kapuganti
language : en
Publisher: Humana
Release Date : 2019-10-15
Nitrogen Metabolism In Plants written by Jagadis Gupta Kapuganti and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-15 with Science categories.
This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field.
Nitric Oxide Protocols
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Author : M. A Titheradge
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Nitric Oxide Protocols written by M. A Titheradge and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
Michael Titheradge provides both the experienced researcher and the newcomer with time-tested techniques to cover all aspects of NO synthase and metabolism. Topics range from the cloning and expression of the different isoforms of NO synthase to the role of NO in DNA damage and apoptosis and include indirect and direct measurements of NO production, measurement on NO synthase mRNA both in situ and in vitro, and Western blotting and immunohistochemical localization of different isoforms of NO synthase. A variety of alternative techniques are described to enable researchers to set up an assay using either the simplest equipment or the most expensive EPR machines.
Plant Stress Tolerance
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Author : Ramanjulu Sunkar
language : en
Publisher: Springer Nature
Release Date :
Plant Stress Tolerance written by Ramanjulu Sunkar and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Plant Mitochondria
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Author : James Whelan
language : en
Publisher: Humana
Release Date : 2015-04-25
Plant Mitochondria written by James Whelan and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-04-25 with Science categories.
The chapters compiled in this detailed collection outline a number of methods used to study plant mitochondria today, starting from the isolation of mitochondria to detailed analyses of RNA, protein and enzymatic activities. Given that the ability to uncover mitochondria’s unique features is underpinned by current methodology, this book explores the subject from morphology to detailed molecular mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Mitochondria: Methods and Protocols serves as a vital resource to beginners in the field as well as to expert researchers who find themselves being pulled into the field of mitochondrial research as it links to so many important aspects of plant cell biology.
Nitric Oxide In Plants
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Author : Juan B. Barroso
language : en
Publisher: Frontiers Media SA
Release Date : 2021-08-26
Nitric Oxide In Plants written by Juan B. Barroso and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-08-26 with Science categories.
Nitric Oxide Signaling In Plants
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Author : John T. Hancock
language : en
Publisher: MDPI
Release Date : 2021-01-28
Nitric Oxide Signaling In Plants written by John T. Hancock and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-28 with Science categories.
This Special Issue is a collection of research articles focused on the production and role of nitric oxide in plants. Nitric oxide is a crucial molecule used in the orchestration of cellular events in animals and plants. With a mixture of primary research papers and review articles written by some of the top researchers in the field, this work encompasses many aspects of this important and growing area of biochemistry.
Plant Salt Tolerance
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Author : Sergey Shabala
language : en
Publisher: Humana Press
Release Date : 2012-08-16
Plant Salt Tolerance written by Sergey Shabala and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-08-16 with Science categories.
Soil salinity is destroying several hectares of arable land every minute. Because remedial land management cannot completely solve the problem, salt tolerant crops or plant species able to remove excessive salt from the soil could contribute significantly to managing the salinity problem. The key to engineering crops for salt tolerance lies in a thorough understanding of the physiological mechanisms underlying the adaptive responses of plants to salinity. Plant Salt Tolerance: Methods and Protocols describes recent advances and techniques employed by researchers to understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms of salt stress perception and signalling in plants. With chapters written by leading international scientists, this book covers nearly 30 different methods, such as microelectrode and molecular methods, imaging techniques, as well as various biochemical assays. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Salt Tolerance: Methods and Protocols serves as an essential read for every student or researcher tackling various aspects of the salinity problem.
Nuclease Methods And Protocols
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Author : Catherine H. Schein
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Nuclease Methods And Protocols written by Catherine H. Schein and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Nucleases, enzymes that restructure or degrade nucleic acid polymers, are vital to the control of every area of metabolism. They range from “housekeeping” enzymes with broad substrate ranges to extremely specific tools (1). Many types of nucleases are used in lab protocols, and their commercial and clinical uses are expanding. The purpose of Nuclease Methods and Protocols is to introduce the reader to some we- characterized protein nucleases, and the methods used to determine their activity, structure, interaction with other molecules, and physiological role. Each chapter begins with a mini-review on a specific nuclease or a nuclease-related theme. Although many chapters cover several topics, they were arbitrarily divided into five parts: Part I, “Characterizing Nuclease Activity,” includes protocols and assays to determine general (processive, distributive) or specific mechanisms. Methods to assay nuclease products, identify cloned nucleases, and determine their physiological role are also included here. Part II, “Inhibitors and Activators of Nucleases,” summarizes assays for measuring the effects of other proteins and small molecules. Many of these inhibitors have clinical relevance. Part III, “Relating Nuclease Structure and Function,” provides an overview of methods to determine or model the 3-D structure of nucleases and their complexes with substrates and inhibitors. A 3-D structure can greatly aid the rational design of nucleases and inhibitors for specific purposes. Part IV, “Nucleases in the Clinic,” summarizes assays and protocols suitable for use with t- sues and for nuclease based therapeutics.