Practical Flow Cytometry In Haematology

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Practical Flow Cytometry In Haematology

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Author : Mike Leach
language : en
Publisher: John Wiley & Sons
Release Date : 2015-06-01

Practical Flow Cytometry In Haematology written by Mike Leach and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-06-01 with Medical categories.

The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May−Grünwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: Provides a practical, example-based resource for flow cytometry Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care Includes both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.

Practical Flow Cytometry In Haematology Diagnosis

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Author : Mike Leach
language : en
Publisher: John Wiley & Sons
Release Date : 2013-04-22

Practical Flow Cytometry In Haematology Diagnosis written by Mike Leach and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-22 with Medical categories.

The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately. Flow cytometry is key in this diagnostic pathway. This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams. The authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry. Practical Flow Cytometry in Haematology Diagnosis provides: A clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditionsCarefully chosen real-life cases in each chapter, complemented by high quality morphological images Help in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfalls All those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide.

Practical Flow Cytometry

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Author : Howard M. Shapiro
language : en
Publisher: John Wiley & Sons
Release Date : 2005-02-25

Practical Flow Cytometry written by Howard M. Shapiro and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-02-25 with Science categories.

From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.

Flow Cytometry Of Hematological Malignancies

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Author : Claudio Ortolani
language : en
Publisher: John Wiley & Sons
Release Date : 2021-04-19

Flow Cytometry Of Hematological Malignancies written by Claudio Ortolani and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-04-19 with Medical categories.

Flow Cytometry of Hematological Malignancies Flow cytometric analysis is often integral to the swift and accurate diagnosis of leukemias and lymphomas of the blood, bone marrow, and lymph nodes. However, in the fast-moving and expanding field of clinical hematology, in can be challenging to remain up to speed with the latest biological research and technological innovations. Flow Cytometry of Hematological Malignancies has been designed to provide all those working in hematological oncology with a practical, cutting-edge handbook, featuring clear and fully illustrated guidance on all aspects of cytometry’s role in diagnosis and analysis. This essential second edition includes: Explorations of more than 70 antigens Full-color illustrations throughout New descriptions of recently discovered markers WHO classifications of hematological neoplastic diseases Helpful tips for result interpretation and analysis Featuring all this and more, Flow Cytometry of Hematological Malignancies, Second Edition, is an invaluable resource for both trainee and experienced hematologists, hematopathologists, oncologists, and pathologists, as well as medical students and diagnostic lab technicians.

Flow Cytometry In Neoplastic Hematology

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Author : Wojciech Gorczyca
language : en
Publisher: CRC Press
Release Date : 2017-07-06

Flow Cytometry In Neoplastic Hematology written by Wojciech Gorczyca and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-06 with Medical categories.

This third edition is the product of the author’s 25 years of experience with flow cytometry; although it covers the wide spectrum of hematopoietic tumors, the focus remains on most important clinical diagnoses, such as acute promyelocytic leukemia, identification of blasts, identification of clonal B-cell population, differentiating mature versus immature T-cell proliferations, deferential diagnosis between hematogones and B-ALL or distinction between chronic and acute monocytic proliferations. All hematopathologists and neoplastic hematologists will find this an important resource for keeping up to date with developments in clinical practice.

Flow Cytometry

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Author : Marion G. Macey
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-11-03

Flow Cytometry written by Marion G. Macey and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-03 with Medical categories.

Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.

Multiparameter Flow Cytometry In The Diagnosis Of Hematologic Malignancies

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Author : Anna Porwit
language : en
Publisher: Cambridge University Press
Release Date : 2018-01-25

Multiparameter Flow Cytometry In The Diagnosis Of Hematologic Malignancies written by Anna Porwit and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-25 with Medical categories.

This practical manual offers an active understanding of how to implement flow-cytometry when facing complex, haematological diseases.

Flow Cytometry In Neoplastic Hematology

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Author : Wojciech Gorczyca
language : en
Publisher: CRC Press
Release Date : 2010-02-25

Flow Cytometry In Neoplastic Hematology written by Wojciech Gorczyca and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-02-25 with Medical categories.

This highly illustrated, practical guide contains comprehensive coverage of all the important factors for clinical diagnosis with flow cytometry. It explains the general parameters and correlation with color histomorphological findings throughout, taking a systematic approach from basic cases to complicated problem areas. Hematopathologists and neoplastic hematologists will find this book an important resource for keeping up to date with developments in clinical practice. This second edition includes a chapter on antigen expression during myeloid and lymphoid differentiation.

Laboratory Hematology Practice

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Author : Kandice Kottke-Marchant
language : en
Publisher: John Wiley & Sons
Release Date : 2012-06-06

Laboratory Hematology Practice written by Kandice Kottke-Marchant and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-06 with Medical categories.

Expertly edited and endorsed by the International Society for Laboratory Hematology, this is the newest international textbook on all aspects of laboratory hematology. Covering both traditional and cutting-edge hematology laboratory technology this book emphasizes international recommendations for testing practices. Illustrative case studies on how technology can be used in patient diagnosis are included. Laboratory Hematology Practice is an invaluable resource for all those working in the field.

Flow Cytometry In Hematopathology

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Author : Doyen T. Nguyen
language : en
Publisher: Springer Science & Business Media
Release Date : 2002-11-26

Flow Cytometry In Hematopathology written by Doyen T. Nguyen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-11-26 with Medical categories.

Flow cytometry immunophenotyping of hematopoietic disorders is a complex and demanding exercise that requires a good understanding of cell lineages, developmental pathways, and physiological changes, as well as broad experience in hematopathology. The process includes several interrelated stages, from the initial medical decision regarding which hematologic c- dition is appropriate for FCM assay, to the final step of diagnosis whereby the FCM data is correlated with other relevant clinical and laboratory information. The actual FCM testing involves three major steps: pre-analytical (specimen processing, antibody staining), analytical (acquiring data on the flow cytometer) and post-analytical (data analysis and interpretation). The literature, including the latest FCM textbooks, provides ample information on the te- nical principles of FCM such as instrumentation, reagents and laboratory methods, as well as quality control and quality assurance. Similarly, correlations of morphologic findings and p- notypic profiles have been well covered in many publications. In contrast, much less attention has been given to the other equally important aspects of FCM immunophenotyping, especially data analysis. The latter is a crucial step by which a phenotypic profile is established. To bridge this gap in the literature, the focus of this book is more on FCM data analysis than laboratory methods and technical details. For the reader to become familiar with our data analysis strategy, an overview of our approach to the pre-analytical and analytical steps is also presented, with an emphasis on the pre-analytical aspects, which have been rarely touched upon in the literature.