Practical Flow Cytometry In Haematology Diagnosis
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Practical Flow Cytometry In Haematology
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Author : Mike Leach
language : en
Publisher: John Wiley & Sons
Release Date : 2015-06-01
Practical Flow Cytometry In Haematology written by Mike Leach and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-06-01 with Medical categories.
The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis. The reader will review May−Grünwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference. Practical Flow Cytometry in Haematology: 100 Worked Examples: Provides a practical, example-based resource for flow cytometry Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care Includes both malignant and benign conditions Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.
Practical Flow Cytometry In Haematology Diagnosis
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Author : Mike Leach
language : en
Publisher: John Wiley & Sons
Release Date : 2013-04-22
Practical Flow Cytometry In Haematology Diagnosis written by Mike Leach and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-22 with Medical categories.
The accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately. Flow cytometry is key in this diagnostic pathway. This book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams. The authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry. Practical Flow Cytometry in Haematology Diagnosis provides: A clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditionsCarefully chosen real-life cases in each chapter, complemented by high quality morphological images Help in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfalls All those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide.
Multiparameter Flow Cytometry In The Diagnosis Of Hematologic Malignancies
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Author : Anna Porwit
language : en
Publisher: Cambridge University Press
Release Date : 2018-01-25
Multiparameter Flow Cytometry In The Diagnosis Of Hematologic Malignancies written by Anna Porwit and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-25 with Medical categories.
This practical manual offers an active understanding of how to implement flow-cytometry when facing complex, haematological diseases.
Flow Cytometry Of Hematological Malignancies
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Author : Claudio Ortolani
language : en
Publisher: John Wiley & Sons
Release Date : 2021-04-19
Flow Cytometry Of Hematological Malignancies written by Claudio Ortolani and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-04-19 with Medical categories.
Flow Cytometry of Hematological Malignancies Flow cytometric analysis is often integral to the swift and accurate diagnosis of leukemias and lymphomas of the blood, bone marrow, and lymph nodes. However, in the fast-moving and expanding field of clinical hematology, in can be challenging to remain up to speed with the latest biological research and technological innovations. Flow Cytometry of Hematological Malignancies has been designed to provide all those working in hematological oncology with a practical, cutting-edge handbook, featuring clear and fully illustrated guidance on all aspects of cytometry’s role in diagnosis and analysis. This essential second edition includes: Explorations of more than 70 antigens Full-color illustrations throughout New descriptions of recently discovered markers WHO classifications of hematological neoplastic diseases Helpful tips for result interpretation and analysis Featuring all this and more, Flow Cytometry of Hematological Malignancies, Second Edition, is an invaluable resource for both trainee and experienced hematologists, hematopathologists, oncologists, and pathologists, as well as medical students and diagnostic lab technicians.
Flow Cytometry In Evaluation Of Hematopoietic Neoplasms
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Author : Sindhu Cherian
language : en
Publisher:
Release Date : 2012
Flow Cytometry In Evaluation Of Hematopoietic Neoplasms written by Sindhu Cherian and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Medical categories.
Flow Cytometry in Evaluation of Hematopoietic Neoplasms: A Case-Based Approach is a practical, case-based guide to flow cytometric analysis in the workup of hematopoietic neoplasms presenting in the peripheral blood, marrow, lymphoid tissue, and extranodal sites. Using multi-color techniques pioneered by Brent Wood, the text demonstrates a unique approach to diagnosis of hematopoietic malignancies as well as identification of small abnormal populations in the posttherapy setting (minimal residual disease testing). The publication contains an introduction to immunophenotypic changes seen in normal hematopoiesis along with an overview of the evaluation of lymphomas, leukemias, and myeloid stem- cell neoplasms. These concepts are further illustrated by a series of 36 cases, each dedicated to a specific disease entity. Each case provides detailed, full-color images of flow cytometric dot plots that clearly outline the features of the disease, accompanied by a clinical history and thorough discussion, enabling readers to develop and work through a differential diagnosis and recognize potential diagnostic pitfalls. Take-home points for each case emphasize critical concepts in flow cytometric diagnosis. Flow Cytometry in Evaluation of Hematopoietic Neoplasms provides pathologists, residents, laboratory technologists, and hematologists with both a study guide and an atlas for regular consultation in the clinical flow cytometry laboratory. The 2008 World Health Organization (WHO) classification system, French-American-British (FAB) classifications, and 2006 Bethesda Consensus recommendations are incorporated in the text.
Flow Cytometry In Neoplastic Hematology
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Author : Wojciech Gorczyca
language : en
Publisher: CRC Press
Release Date : 2010-02-25
Flow Cytometry In Neoplastic Hematology written by Wojciech Gorczyca and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-02-25 with Medical categories.
This highly illustrated, practical guide contains comprehensive coverage of all the important factors for clinical diagnosis with flow cytometry. It explains the general parameters and correlation with color histomorphological findings throughout, taking a systematic approach from basic cases to complicated problem areas. Hematopathologists and neoplastic hematologists will find this book an important resource for keeping up to date with developments in clinical practice. This second edition includes a chapter on antigen expression during myeloid and lymphoid differentiation.
Practical Flow Cytometry
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Author : Howard M. Shapiro
language : en
Publisher: John Wiley & Sons
Release Date : 2005-02-25
Practical Flow Cytometry written by Howard M. Shapiro and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-02-25 with Science categories.
From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.
Flow Cytometry
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Author : Marion G. Macey
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-11-03
Flow Cytometry written by Marion G. Macey and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-03 with Medical categories.
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.
Laboratory Hematology Practice
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Author : Kandice Kottke-Marchant
language : en
Publisher: John Wiley & Sons
Release Date : 2012-06-06
Laboratory Hematology Practice written by Kandice Kottke-Marchant and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-06 with Medical categories.
Expertly edited and endorsed by the International Society for Laboratory Hematology, this is the newest international textbook on all aspects of laboratory hematology. Covering both traditional and cutting-edge hematology laboratory technology this book emphasizes international recommendations for testing practices. Illustrative case studies on how technology can be used in patient diagnosis are included. Laboratory Hematology Practice is an invaluable resource for all those working in the field.
Cellular Diagnostics
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Author : Ulrich Sack
language : en
Publisher: Karger Medical and Scientific Publishers
Release Date : 2009-01-01
Cellular Diagnostics written by Ulrich Sack and has been published by Karger Medical and Scientific Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-01 with Medical categories.
This book is the updated English version of the 2006 German bestseller Zellulare Diagnostik, a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance.