Protein Engineering Techniques
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Protein Engineering Techniques
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Author : Krishna Mohan Poluri
language : en
Publisher: Springer
Release Date : 2016-11-12
Protein Engineering Techniques written by Krishna Mohan Poluri and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-11-12 with Technology & Engineering categories.
This brief provides a broad overview of protein-engineering research, offering a glimpse of the most common experimental methods. It also presents various computational programs with applications that are widely used in directed evolution, computational and de novo protein design. Further, it sheds light on the advantages and pitfalls of existing methodologies and future perspectives of protein engineering techniques.
A Practical Guide To Protein Engineering
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Author : Tuck Seng Wong
language : en
Publisher: Springer Nature
Release Date : 2020-10-29
A Practical Guide To Protein Engineering written by Tuck Seng Wong and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-29 with Science categories.
This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
Protein Engineering Protocols
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Author : Kristian Müller
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26
Protein Engineering Protocols written by Kristian Müller and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Science categories.
Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.
Protein Engineering And Design
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Author : Paul R. Carey
language : en
Publisher: Elsevier
Release Date : 1996-06-21
Protein Engineering And Design written by Paul R. Carey and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-06-21 with Science categories.
The design and production of novel peptides and proteins occupy pivotal positions in science and technology and will continue to do so in the 21st century. Protein Engineering and Design outlines the rapid advances in computer-based modeling, protein engineering, and methods needed for protein and peptide preparation and characterization. This indispensable reference lays the groundwork for understanding this multidisciplinary activity while providing an introduction for researchers and students to the field of protein design. - Introduces and defines the techniques involved in protein engineering and design - Provides a concise overview of key technologies involved and demonstrates their contributions to the specialized design and production of novel proteins and peptides
Protein Engineering And Design
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Author : Sheldon J. Park
language : en
Publisher: CRC Press
Release Date : 2009-09-25
Protein Engineering And Design written by Sheldon J. Park and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-09-25 with Medical categories.
Experimental protein engineering and computational protein design are broad but complementary strategies for developing proteins with altered or novel structural properties and biological functions. By describing cutting-edge advances in both of these fields, Protein Engineering and Design aims to cultivate a synergistic approach to protein science
Enzymes For Biotechnology
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Author :
language : en
Publisher:
Release Date : 1988
Enzymes For Biotechnology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with categories.
Enzyme Engineering
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Author : James Samuelson
language : en
Publisher: Humana Press
Release Date : 2016-05-01
Enzyme Engineering written by James Samuelson and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with Science categories.
This book provides guidance to those wishing to create enzyme variants. It covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes and the engineering of two difference types of rare-cutting endonucleases.
Protein Engineering
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Author : Jeffrey L. Cleland
language : en
Publisher: Wiley-Liss
Release Date : 1996-02-07
Protein Engineering written by Jeffrey L. Cleland and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-02-07 with Science categories.
This systematic approach to protein engineering provides a thorough introduction to protein biophysics via chapters dealing with the experimental and theoretical determination of protein structure. Separate chapters discuss each of the expression systems c
Protein Engineering
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Author : Uwe T. Bornscheuer
language : en
Publisher: Humana Press
Release Date : 2017-10-30
Protein Engineering written by Uwe T. Bornscheuer and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-30 with Science categories.
This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.
Medicinal Protein Engineering
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Author : Yury E. Khudyakov
language : en
Publisher: CRC Press
Release Date : 2008-12-01
Medicinal Protein Engineering written by Yury E. Khudyakov and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-12-01 with Medical categories.
An All-Inclusive Review of the Achievements and Trends in the Fast-Growing Protein Engineering Field From humble beginnings like making fire for mere survival, engineering now steadfastly penetrates all aspects of our lives and even life itself at the molecular level. Protein engineering is a molecular biological discipline focused on designing and