Protein Expression Technologies
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Protein Expression Technologies
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Author : François Baneyx
language : en
Publisher: Garland Science
Release Date : 2004
Protein Expression Technologies written by François Baneyx and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Medical categories.
Advances in protein expression technologies have mushroomed in recent years. In this book current and emerging expression technologies are reviewed. Reviews of the molecular genetics of expression systems in various organisms are presented. Topics covered include: Expression of extremophilic proteins; expression in E. coli, Bacillus spp., Saccharomyces cerevisiae, and methylotrophic yeasts; insect cell expression and the baculovirus system; and Chinese Hamster Ovary (CHO) cell lines for large-scale protein production. Also covered are two emerging expression systems, Methylobacterium extorquens AM1 and Caulobacter crescentus.
Advanced Technologies For Protein Complex Production And Characterization
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Author : M. Cristina Vega
language : en
Publisher: Springer
Release Date : 2016-05-10
Advanced Technologies For Protein Complex Production And Characterization written by M. Cristina Vega and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-10 with Science categories.
This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.
Production Technology Of Recombinant Therapeutic Proteins
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Author : Chiranjib Chakraborty
language : en
Publisher: Daya Books
Release Date : 2004
Production Technology Of Recombinant Therapeutic Proteins written by Chiranjib Chakraborty and has been published by Daya Books this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Biotechnology categories.
An Increasing Number Of Recombinant Therapeutic Proteins Are Currently Being Developed, Tested In Clinical Trials And Marketed For Used. Most Of The Recombinant Therapeutic Proteins Are Being Successfully Produced Into Escherichia Coli And Pichia Pastoris Expression System. These Two Expression Systems Are Very Much Efficient And Cost Effective. This Book Takes A Close Look Of These Two Expression Systems And Fermentation Conditions, Purification Strategies Of Different Recombinant Proteins. This Book Also Discusses The Market Size And Cost Analysis For The Production Of Different Therapeutic Proteins And Some General Experimental Protocols For Production. Contents Part I: Recombinant Protein Expression Into Escherichia Coli And Fermentation Conditions; Chapter 1: Introduction; Chapter 2: Construction Of Efficient Expression Vector (Plasmid); Chapter 3: Factors Affecting Transcription, Promoters, Upstream Elements, Transcriptional Terminators, Transcriptional Antitermin, Tightly Regulated Expression Systems; Chapter 4: Mrna Stability; Chapter 5: Factors Affecting Translation, Mrna Translational Initiator, Translational Enhancers, Translational Termination; Chapter 6: Expression Of Target Protein And The Compartments Of Expression, Cytoplasmic Expression, Periplasmic Expression, Extracellular Secretion; Chapter 7: Fusion Proteins; Chapter 8: Post-Translational Protein Folding; Chapter 8: Codon Usage; Chapter 10: Protein Degradation; Chapter 11: Fermentation Conditions For High-Density Cell Cultivation (Hdcc), Growth Medium, Efficient Production Of Recombinant Protein In Hdcc, Nutrient Feeding Strategy In Hdcc; Chapter 12: One Examples Of Protein Production Using E. Coli Expression System; Chapter 13: Conclusion. Part Ii: Recombinant Protein Expression Into Yeast, Pichia Pastoris And Fermentation Conditions; Chapter 1: Introduction; Chapter 2: Why P. Pastoris? Chapter 3: Construction Of Expression Strains, Expression Vectors, Alternative Promoters, Host Strains, Methanol Utilisation Phenotype, Protease-Reduced Host Strains, Integration Of Expression Vectors Into The P. Pastoris Genome, Generating Multicopy Strains; Chapter 4: Post-Translational Modifications Of Secreted Proteins, Secretion Signal Selection, N-Linked Glycosylation; Chapter 5: Production Of Recombinant Proteins In Fermenter Cultures Of The Yeast, Pichia Pastoris, Conceptual Basis For The P. Pastoris Expression System, High-Level Expression In Fermenter Cultures, Protein-Specific Adjustments To Improve Yield, Glycosylation Of Recombinant Proteins, Secretion Signals; Chapter 6: One Examples Of Protein Producing Using P. Pastoris Expression System, Chapter 7: Conclusion. Part Iii: Purification Strategies For Recombinant Proteins; Chapter 1: Purification Of Proteins; Chapter 2: Conventional Chromatography, Ion Exchange Chromatography, Reversed Phase Chromatography, Gel Permeation Chromatography, Affinity Chromatography, Affinity Tags, Cleavage, Conclusion. Part Iv: Market Size And Cost Analysis For The Production Of Therapeutic Proteins; Chapter 1: Market Size Of Therapeutic Proteins; Chapter 2: Outline Structure Of A Productin Unit And Cost Analysis For The Production Of Three Therapeutic Proteins. Part V: General Experimental Protocols; Chapter 1: Different Experimental Protocols, Preparation Of Genome Dna For E. Coli, A Differnt Method For Preparation Of Genomic Dna From Bacteria, Preparation Of Proteins From Periplasm (Osmotic Shock Method), Preparation Of Proteins From Outer Membrane, Transformation Of Plasmid Dna Into E. Coli (Calcium Chloride/Heat Shock Method), Transformation Of Plasmid Dna Into E. Coli (Electroporation), Sds-Page For Large Proteins, Sds-Page For Small Peptide, Pcr Amplification Of Dna, Protein Quantification: Brandford Method, Trans-Bloting For Protein, Restriction Enzyme Digestion Of Dna, Phenol/Chloroform Extraction Of Dna, Ethanol Precipitation Of Dna, Agarose Gel Electrophoresis, Transformation Of E. Coli By Electroporation (Alternative Method), Wizard Tm Pcr Preps Dna Purification System For Rapid, Purification Of Dna Fragments, Alternate Method For Purifying Dna From Agarose Gels, Southern Blotting, Rt Pcr Protocol, Using Superscript Reverse Transcriptase, Preparation Of Sequencing Gels, Isolation Of Rna From Mammalian Cells Using Rnazoltm (Teltest), Preparation For Yeast Transformation, Yeast Transformation, Digesting Prsq-Ura3 With Bamhi, Genomic Dna Preparation Of Yeast, Ligation (Circularisation) Of Genomic Dna Fragments, E. Coli Transformation (Alternate Method), Dna Miniprep From E. Coli (Alternate Method), Basic Plasmid Dna Isolation Protocol, Identification And Determination Of Amount Rec-Hum Proteins Via An Immunoenzymatic Test (Elisa), Determination Of Host Dna Contaminant Into R Hu Protein Through Dot Blot Method, Protocols For Down-Stream Processing.
Recombinant Protein Expression In Mammalian Cells
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Author : David L. Hacker
language : en
Publisher: Humana
Release Date : 2024-08-17
Recombinant Protein Expression In Mammalian Cells written by David L. Hacker and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-08-17 with Science categories.
This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell surface protein ectodomain expression and for identifying host genes involved in protein production. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols, Second Edition serves as an ideal guide for researchers investigatingprotein structure and function and accelerating the discovery of new therapeutic proteins.
Gene Cloning And Expression Technologies
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Author : Michael P. Weiner
language : en
Publisher: Biotechniques Press
Release Date : 2002
Gene Cloning And Expression Technologies written by Michael P. Weiner and has been published by Biotechniques Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Medical categories.
Gene Expression Technology
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Author : David V. Goeddel
language : en
Publisher:
Release Date : 1990
Gene Expression Technology written by David V. Goeddel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Gene Expression categories.
The methods presented in this volume will enable the reader to design effective strategies for the expression of cloned genes and cDNAs and will prove useful in solving the majority of expression problems one is likely to encounter.
Gene Expression Systems
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Author : Joseph M. Fernandez
language : en
Publisher: Elsevier
Release Date : 1998-12-21
Gene Expression Systems written by Joseph M. Fernandez and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-12-21 with Science categories.
Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. Provides detailed information on expression systems Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs Includes timelines and examples Compares pros and cons of each method
Cell Free Protein Expression
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Author : W. Antoni Kudlicki
language : en
Publisher: CRC Press
Release Date : 2007-11-27
Cell Free Protein Expression written by W. Antoni Kudlicki and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-27 with Science categories.
Following its inception in the 1950s, cell-free protein synthesis made a tremendous impact on the basic life sciences. The use of cell-free systems was key to understanding molecular mechanisms underlying one of the most complicated processes found in nature: protein translation. Since this time, aggressive cutting-edge research and stiff commerical competition have driven the development of a variety of systems with increased productivity, improved protein quality and relatively low production costs. As a result, technology has generated myriad applications that have enabled advances in fields as diverse as systems biology, structural biology, and drug discovery. Cell-Free Protein Expression describes and expands upon many of these applications. The volume has been divided into six main sections. In the first section, many of the most popular sources of cell-free lysates are introduced. The second section focuses on extraordinary advances made in the Escherichia coli-based systems that have enabled reconstitution of the entire translational process, incorporation of post-translational modifications, yield increase, and production of functional membrane proteins. This progress extends the usefulness of cell-free systems into structural biology applications described in the third section and high-content platforms like protein microarrays discussed in the fourth section. The final two sections cover the use of cell-free protein expression technologies in the rational design and directed evolution of proteins within the scientific community.
Cell Free Protein Expression
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Author : James R. Swartz
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Cell Free Protein Expression written by James R. Swartz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Cell-free protein synthesis is coming of age! Motivated by an escalating need for efficient protein synthesis and empowered by readily accessible cell-free protein synthesis kits, the technology is expanding both in the range of feasible proteins and in the ways that proteins can be labeled and modified. This volume follows "Cell-Free Translation Systems", edited by Professor Alexander S. Spirin in 2002. Since then, an impressive collection of new work has emerged that demonstrates a substantial expansion of capability. In this volume, we show that proteins now can be efficiently produced using PCR products as DNA templates and that even membrane proteins and proteins with multiple disulfide proteins are obtained at high yields. Many additional advances are also presented. It is an exciting time for protein synthesis technology.
Protein Expression In Mammalian Cells
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Author : James L Hartley
language : en
Publisher: Humana Press
Release Date : 2011-10-12
Protein Expression In Mammalian Cells written by James L Hartley and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-12 with Science categories.
Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.