Proteoglycan Protocols
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Proteoglycan Protocols
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Author : Renato V. Iozzo
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Proteoglycan Protocols written by Renato V. Iozzo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Proteoglycans are some of the most elaborate macromolecules of mammalian and lower organisms. The covalent attachment of at least five types of glycosami- glycan side chains to more than forty individual protein cores makes these molecules quite complex and endows them with a multitude of biological functions. Proteoglycan Protocols offers a comprehensive and up-to-date collection of prepa- tive and analytical methods for the in-depth analysis of proteoglycans. Featuring st- by-step detailed protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, to generate strategies for prokaryotic and eukaryotic expression, to utilize methods for the suppression of specific proteoglycan gene expression and for the detection of mutant cells and degradation products, and to study specific interactions between proteoglycans and extracellular matrix proteins as well as growth factors and their receptors. The readers will find concise, yet comprehensive techniques carefully drafted by leading experts in the field. Each chapter commences with a general Introduction, followed by a detailed Materials section, and an easy-to-follow Methods section. An asset of each chapter is the extensive notation that includes troubleshooting tips and practical considerations that are often lacking in formal methodology papers. The reader will find this section most valuable because it is clearly provided by experienced scientists who have first-hand knowledge of the techniques they outline. In addition, most of the chapters are well illustrated with examples of typical data generated with each method.
The Protein Protocols Handbook
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Author : John M. Walker
language : en
Publisher: Springer
Release Date : 2007-10-02
The Protein Protocols Handbook written by John M. Walker and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-02 with Science categories.
The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Methods In Cell Matrix Adhesion
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Author : Josephine Adams
language : en
Publisher: Academic Press
Release Date : 2002-06-12
Methods In Cell Matrix Adhesion written by Josephine Adams and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-06-12 with Science categories.
Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This volume, Methods of Cell-Matrix Adhesion, contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This coverage includes experimental protocols and their conceptual background for all aspects of cell-matrix adhesion research: the extracellular matrix, adhesion receptors, and the growing number of functional applications of matrix-adhesion in molecular cell biology. Also covered is the purification of the extracellular matrix to functional analyses of cellular responses.
Proteoglycans
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Author : Nikos K. Karamanos
language : en
Publisher: Springer Nature
Release Date : 2023-01-20
Proteoglycans written by Nikos K. Karamanos and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-20 with Science categories.
This volume is a comprehensive and up-to-date collection of strategies, reproducible methods, and protocols for the in-depth analysis of Proteoglycans (PGs) and their glycan part, the GAGs. Chapters are divided into three parts detailing GAGs in biological specimens, protocols for the evaluation of the in vitro and in vivo effects of PGs/GAGs, and protocols for compounds related with the metabolic enzymes, epigenetic regulation, and PGs/GAGs-based inhibitors. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Cutting-edge and thorough, Proteoglycans: Methods and Protocols aims to provide information on the elucidated the structural and functional aspects of the complex matrix macromolecules such as the proteoglycans and glycosaminoglycans.
Glycoanalysis Protocols
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Author : Elizabeth F. Hounsell
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Glycoanalysis Protocols written by Elizabeth F. Hounsell and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Glycoanalysis Protocols, Second Edition, makes available to all protein scientists, and particularly those working in today's pharmaceuticals industry, the most advanced and reproducible glycoanalysis techniques. These detailed, up-to-date, and proven analytical methods cover the areas of glycoprotein macromolecular structural analysis, oligosaccharide profiling, lipid conjugate characterization, microorganism structure determination, and proteoglycan function. They also include advanced analytical techniques in biotechnology during the production of recombinant glycoproteins and other therapeutics. These protocols will well serve anyone starting work on the analysis of glycoproteins, as well as experienced investigators seeking to carry their expertise to higher levels of accomplishment.
Tumor Suppressor Genes
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Author : Wafik S. El-Deiry
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Tumor Suppressor Genes written by Wafik S. El-Deiry and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Medical categories.
It has become clear that tumors result from excessive cell proliferation and a corresponding reduction in cell death caused by the successive accumulation of mutations in key regulatory target genes over time. During the 1980s, a number of oncogenes were characterized, whereas from the 1990s to the present, the emp- sis has shifted to tumor suppressor genes (TSGs). It has become clear that oncogenes and TSGs function in the same pathways, providing positive and negative growth regulatory activities. The signaling pathways controlled by these genes involve virtually every process in cell biology, including nuclear events, cell cycle, cell death, cytoskeletal, cell membrane, angiogenesis, and cell adhesion effects. Mu- tions in tumor suppressor genes have been identified in familial cancer syndromes, and the same genes in many cases have been found to be mutationally inactivated in sporadically occurring cancers. In their normal state, TSGs control cancer development and progression, as well as contribute to the sensitivity of cancers to a variety of therapeutics. Understanding the classes of TSGs, the biochemical pa- ways they function in, and how they are regulated provides an essential lesson in cancer biology. We cannot hope to advance our current knowledge and to develop new and more effective therapies without understanding the relevant pathways and how they influence the present approaches to therapy. Moreover, it is important to be able to access not only the powerful tools now available to discover these genes, but also their links to cell biology and growth control.
Biology Of Proteoglycans
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Author : Thomas N. Wight
language : en
Publisher: Elsevier
Release Date : 2013-09-11
Biology Of Proteoglycans written by Thomas N. Wight and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-09-11 with Science categories.
Biology of Proteoglycans provides a representative, but by no means inclusive, sample of current research on the role of proteoglycans in the cell biology of the extracellular matrix. The book is organized into four areas: methodological developments; proteoglycan metabolism; proteoglycans in cartilage; and proteoglycans in "soft" tissues. It begins with discussions of current methodological developments which have had enormous impact on understanding the complexity of proteoglycan structure. These include the use of monoclonal antibodies to probe the structure of the protein and carbohydrate portions of proteoglycans; and studies on the genes that code for the proteoglycan protein cores and associated proteins. This is followed by separate chapters on various aspects of proteoglycan metabolism, including the synthetic pathway used by cells to synthesize proteoglycans containing heparin and heparan sulfate; the molecular organization of different proteoglycans in cartilage; the role of proteoglycans and associated proteins in the calcification process in growth plate; and the emerging field of proteoglycans in "soft" or noncartilagenous tissue.
Marine Carbohydrates Fundamentals And Applications Part A
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Author :
language : en
Publisher: Academic Press
Release Date : 2014-07-29
Marine Carbohydrates Fundamentals And Applications Part A written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-29 with Technology & Engineering categories.
Marine Carbohydrates: Fundamentals and Applications brings together the diverse range of research in this important area which leads to clinical and industrialized products. The volume, number 72, focuses on marine carbohydrates in isolation, biological, and biomedical applications and provides the latest trends and developments on marine carbohydrates. Advances in Food and Nutrition Research recognizes the integral relationship between the food and nutritional sciences and brings together outstanding and comprehensive reviews that highlight this relationship. Volumes provide those in academia and industry with the latest information on emerging research in these constantly evolving sciences. - Includes the isolation techniques for the exploration of the marine habitat for novel polysaccharides - Discusses biological applications such as antioxidant, antiallergic, antidiabetic, antiobesity and antiviral activity of marine carbohydrates - Provides an insight into present trends and approaches for marine carbohydrates
Chemistry And Biology Of Heparin And Heparan Sulfate
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Author : Hari G. Garg
language : en
Publisher: Elsevier
Release Date : 2011-10-10
Chemistry And Biology Of Heparin And Heparan Sulfate written by Hari G. Garg and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-10 with Science categories.
The chemistry, biochemistry and pharmacology of heparin and heparan sulfate have been and continue to be a major scientific undertaking - heparin and its derivative remain important drugs in clinical practice. Chemistry and Biology of Heparin and Heparan Sulfate provides readers with an insight into the chemistry, biology and clinical applications of heparin and heparan sulfate and examines their function in various physiological and pathological conditions. Providing a wealth of useful information, no other tome covers the diversity of topics in the field. Students, doctors, chemists, biochemists, and research scientists will find this book an invaluable source for updating their current knowledge of developments in this area. - Comprehensively reviews all aspects of heparin and heparan sulfate research - Uniquely describes the chemistry, biology and clinical application of heparins and heparan sulfates in one work - Provides an invaluable source of knowledge of current developments for chemists, biochemists, medical doctors, researchers, students and practitioners
Carbohydrate Chemistry State Of The Art And Challenges For Drug Development An Overview On Structure Biological Roles Synthetic Methods And Application As Therapeutics
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Author : Laura Cipolla
language : en
Publisher: World Scientific
Release Date : 2015-07-23
Carbohydrate Chemistry State Of The Art And Challenges For Drug Development An Overview On Structure Biological Roles Synthetic Methods And Application As Therapeutics written by Laura Cipolla and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-07-23 with Science categories.
The structural complexity and the synthetic challenges facing glycans have historically hampered efforts to study their multifaceted roles and the application of carbohydrates in drug development. However, in very recent years, new synthetic techniques flanked by the growing knowledge about carbohydrate involvement in physiological and pathological states has spurred renewed interest in the chemistry, biology and therapeutic potentialities of carbohydrates. This book offers an overview of key aspects of carbohydrate biology and chemistry that are fundamental for the design of novel therapeutics. The four-part structure of this book introduces these essential components to life, starting from their structure and biological roles and covering analytical methods and synthesis which pave the way for the development of a wide range of therapeutic applications.Leading experts from around the world are brought together to offer their recent research with the ultimate aim of enlightening the reader on the complex yet exciting field of carbohydrate chemistry. Academic and industrial researchers in structural biology, drug discovery and carbohydrate chemistry will find this book an essential guide to the latest research and future potential of medicinal chemistry.