Proteomics Sample Preparation
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Proteomics Sample Preparation
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Author : Jörg von Hagen
language : en
Publisher: John Wiley & Sons
Release Date : 2011-08-24
Proteomics Sample Preparation written by Jörg von Hagen and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-24 with Science categories.
This long-awaited first guide to sample preparation for proteomics studies overcomes a major bottleneck in this fast growing technique within the molecular life sciences. By addressing the topic from three different angles -- sample, method and aim of the study -- this practical reference has something for every proteomics researcher. Following an introduction to the field, the book looks at sample preparation for specific techniques and applications and finishes with a section on the preparation of sample types. For each method described, a summary of the pros and cons is given, as well as step-by-step protocols adaptable to any specific proteome analysis task.
Modern Proteomics Sample Preparation Analysis And Practical Applications
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Author : Hamid Mirzaei
language : en
Publisher: Springer
Release Date : 2016-12-14
Modern Proteomics Sample Preparation Analysis And Practical Applications written by Hamid Mirzaei and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-12-14 with Science categories.
This volume serves as a proteomics reference manual, describing experimental design and execution. The book also shows a large number of examples as to what can be achieved using proteomics techniques. As a relatively young area of scientific research, the breadth and depth of the current state of the art in proteomics might not be obvious to all potential users. There are various books and review articles that cover certain aspects of proteomics but they often lack technical details. Subject specific literature also lacks the broad overviews that are needed to design an experiment in which all steps are compatible and coherent. The objective of this book was to create a proteomics manual to provide scientists who are not experts in the field with an overview of: 1. The types of samples can be analyzed by mass spectrometry for proteomics analysis. 2. Ways to convert biological or ecological samples to analytes ready for mass spectral analysis. 3. Ways to reduce the complexity of the proteome to achieve better coverage of the constituent proteins. 4. How various mass spectrometers work and different ways they can be used for proteomics analysis 5. The various platforms that are available for proteomics data analysis 6. The various applications of proteomics technologies in biological and medical sciences This book should appeal to anyone with an interest in proteomics technologies, proteomics related bioinformatics and proteomics data generation and interpretation. With the broad setup and chapters written by experts in the field, there is information that is valuable for students as well as for researchers who are looking for a hands on introduction into the strengths, weaknesses and opportunities of proteomics.
Proteomics Mass Spectrometry Methods
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Author : Paula Meleady
language : en
Publisher: Elsevier
Release Date : 2024-02-13
Proteomics Mass Spectrometry Methods written by Paula Meleady and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-02-13 with Science categories.
Proteomics Mass Spectrometry Methods: Sample Preparation, Protein Digestion, and Research Protocols shares best practices collected across key laboratories and core facilities, taking the reader through key tactics for executing the most usual mass spectrometry experiments. Sections review research making use of MS proteomics experiments, focus on critical sample preparation, cover mammalian cell lines and samples from clinical tissue and biological fluids, discuss subcellular fractionation, provide methods for protein digestion both for in gel and in solution, and delve into key MS proteomics analysis protocols, including label-free LC-MS, TMT and iTRAQ labelled LC-MS, phosphorylation enrichment, ubiquination enrichment, and more. This book is the perfect lab manual for research teams or for use as a new staff training material. Core facility managers may also find it useful for sharing best practices with their staff and researchers. Explores the most common questions new researchers have Guides readers to properly design the workflow for successful integration of mass spectrometry into protein biochemical analyses Provides examples of sample preparation for a number of different materials, mammalian cells, and others
Sample Preparation In Biological Mass Spectrometry
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Author : Alexander R. Ivanov
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-06-15
Sample Preparation In Biological Mass Spectrometry written by Alexander R. Ivanov and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-15 with Science categories.
The aim of this book is to provide the researcher with important sample preparation strategies in a wide variety of analyte molecules, specimens, methods, and biological applications requiring mass spectrometric analysis as a detection end-point. In this volume we have compiled the contributions from several laboratories which are employing mass spectrometry for biological analysis. With the latest inventions and introduction of highly sophisticated mass spectrometry equipment sample preparation becomes an extremely important bottleneck of biomedical analysis. We have a goal of giving the reader several successful examples of sample preparation, development and optimization, leading to the success in analytical steps and proper conclusions made at the end of the day. This book is structured as a compilation of contributed chapters ranging from protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.
2d Page Sample Preparation And Fractionation
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Author : Anton Posch
language : en
Publisher: Humana Press
Release Date : 2010-10-28
2d Page Sample Preparation And Fractionation written by Anton Posch and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-10-28 with Science categories.
This book presents broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and two-dimensional electrophoresis (2-DE) in particular. With its unique capacity to resolve thousands of proteins in a single run, 2-DE is still a fundamental research tool for nearly all protein-related scientific projects.
2d Page Sample Preparation And Fractionation
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Author : Anton Posch
language : en
Publisher: Humana Press
Release Date : 2008-03-19
2d Page Sample Preparation And Fractionation written by Anton Posch and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-19 with Science categories.
This book presents broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and two-dimensional electrophoresis (2-DE) in particular. With its unique capacity to resolve thousands of proteins in a single run, 2-DE is still a fundamental research tool for nearly all protein-related scientific projects.
Tissue Proteomics
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Author : Minnie M. Sarwal
language : en
Publisher:
Release Date : 2018
Tissue Proteomics written by Minnie M. Sarwal and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Proteomics categories.
Emerging Sample Treatments In Proteomics
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Author : José-Luis Capelo-Martínez
language : en
Publisher: Springer
Release Date : 2019-06-24
Emerging Sample Treatments In Proteomics written by José-Luis Capelo-Martínez and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-24 with Science categories.
Proteomics is a well-established area of Science; yet with a strong area in constant evolution, namely sample treatment. There few books that currently cover the field of emerging sample treatments in proteomics, this new volume will be the first to cover all emerging and existing studies. This unique book presents the latest advances in the field focusing on emerging trends linked to high-resolution mass spectrometry, technology addressed to treat samples faster and to attempts to simplify the proteome for the reader.
2d Page Sample Preparation And Fractionation
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Author : Anton Posch
language : en
Publisher: Humana
Release Date : 2008-03-18
2d Page Sample Preparation And Fractionation written by Anton Posch and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-18 with Science categories.
Focusing on two-dimensional electrophoresis (2-DE), this book sets forth the principles and recent developments of sample preparation and fractionation tools in expression proteomics. Step-by-step instructions alongside detailed illustrations enable you to quickly learn how to perform the methods yourself. Tricks of the trade and notes on troubleshooting and avoiding pitfalls help you ensure successful results every time. The methods covered in this book are not limited to 2-DE; they can also be applied to a broad range of protein separation techniques.
Sample Preparation In Biological Mass Spectrometry
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Author : Alexander R. Ivanov
language : en
Publisher: Springer
Release Date : 2013-01-02
Sample Preparation In Biological Mass Spectrometry written by Alexander R. Ivanov and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-01-02 with Science categories.
The aim of this book is to provide the researcher with important sample preparation strategies in a wide variety of analyte molecules, specimens, methods, and biological applications requiring mass spectrometric analysis as a detection end-point. In this volume we have compiled the contributions from several laboratories which are employing mass spectrometry for biological analysis. With the latest inventions and introduction of highly sophisticated mass spectrometry equipment sample preparation becomes an extremely important bottleneck of biomedical analysis. We have a goal of giving the reader several successful examples of sample preparation, development and optimization, leading to the success in analytical steps and proper conclusions made at the end of the day. This book is structured as a compilation of contributed chapters ranging from protocols to research articles and reviews. The main philosophy of this volume is that sample preparation methods have to be optimized and validated for every project, for every sample type and for every downstream analytical technique.