Protocols And Applications In Enzymology
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Protocols And Applications In Enzymology
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Author : Seema Anil Belorkar
language : en
Publisher: Academic Press
Release Date : 2021-11-19
Protocols And Applications In Enzymology written by Seema Anil Belorkar and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-11-19 with Science categories.
Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses. - Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards - Presents easy to read, reproducible protocols for researchers and students across academia and industry - Includes color diagrams that illustrate key concepts
Protocols And Applications In Enzymology
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Author : Seema Anil Belorkar
language : en
Publisher: Elsevier
Release Date : 2021-11-24
Protocols And Applications In Enzymology written by Seema Anil Belorkar and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-11-24 with Science categories.
Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses. Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards Presents easy to read, reproducible protocols for researchers and students across academia and industry Includes color diagrams that illustrate key concepts
Methods In Non Aqueous Enzymology
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Author : Munishwar N. Gupta
language : en
Publisher: Birkhäuser
Release Date : 2013-12-01
Methods In Non Aqueous Enzymology written by Munishwar N. Gupta and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-01 with Science categories.
Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar feature which distinguishes it from the conventional enzymology (carried out in aqueous buffers) is that the awareness of different parameters that control and influence the behaviour of enzymes in such environments has emerged rather slowly. Science is about being able to repeat what somebody else has done. Absence of knowledge about such well-defined parameters/fac tors has sometimes made some workers rather cautious and diffident about using this approach in their laboratories. But for this, non-aqueous enzymol ogy would be more widely practised. It is these thoughts that made me feel that the availability of some well-defined protocols for various applications invol ving enzymes in non-aqueous environments would further catalyze the growth of this area. Hence this book, in which each chapter has some protocols in a specific area. The protocols are preceded by brief background material. The early chapters, which are of general importance, concern control of water ac tivity and stabilization via immobilization. Some subsequent chapters provide the protocols for transformations involving lipids and carbohydrates, peptide synthesis, and preparation of chiral compounds. The disproportionate focus on lipases is not a coincidence; this class of enzymes has been used more often than others in non-aqueous enzymology.
Immobilization Of Enzymes And Cells
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Author : Jose M. Guisan
language : en
Publisher: Humana
Release Date : 2021-01-29
Immobilization Of Enzymes And Cells written by Jose M. Guisan and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-29 with Science categories.
This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.
Industrial Enzyme Applications
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Author : Andreas Vogel
language : en
Publisher: John Wiley & Sons
Release Date : 2019-07-10
Industrial Enzyme Applications written by Andreas Vogel and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-10 with Science categories.
This reference is a "must-read": It explains how an effective and economically viable enzymatic process in industry is developed and presents numerous successful examples which underline the efficiency of biocatalysis.
Practical Enzymology
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Author : Hans Bisswanger
language : en
Publisher: John Wiley & Sons
Release Date : 2019-10-21
Practical Enzymology written by Hans Bisswanger and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-21 with Science categories.
A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.
Enzyme Engineering
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Author : James Samuelson
language : en
Publisher: Humana Press
Release Date : 2016-05-01
Enzyme Engineering written by James Samuelson and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with Science categories.
This book provides guidance to those wishing to create enzyme variants. It covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes and the engineering of two difference types of rare-cutting endonucleases.
Electrophoresis Of Enzymes
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Author : Gunter M. Rothe
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Electrophoresis Of Enzymes written by Gunter M. Rothe and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The electrophoresis of enzymes and isoenzymes is a well established technique in biochemical, clinical, environmental, microbiological, botanical and forensic laboratories and classical electrophoresis is presently undergoing a remarkable revival. This book compiles facts and methods on enzyme electrophoresis widely dispersed in hundreds of publications. The author summarizes them in clearly readable tables, in many carefully worked out electrophoresis and more than 140 staining protocols. The exhaustive practical experience of the author and the wealth of material summarized and reviewed makes this book a "must" for every enzyme laboratory. It will supply the practitioner with profound information on state-of-the-art enzyme electrophoresis.
Enzyme Kinetics In Drug Metabolism
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Author : Swati Nagar
language : en
Publisher: Humana
Release Date : 2014-02-13
Enzyme Kinetics In Drug Metabolism written by Swati Nagar and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-13 with Medical categories.
Drug metabolism and transport are very important facets within the discipline of pharmaceutical sciences, with enzyme kinetic concepts utilized regularly in characterizing and modeling the disposition and elimination of drugs. Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications focuses on very practical aspects of applying kinetic principles to drug metabolizing enzymes and transporters. Divided into five convenient sections, topics include the fundamental principles of enzyme kinetics, the kinetics of oxidative and conjugative drug metabolizing enzymes and drug transporters, modeling approaches for both drug metabolizing enzymes and transporters including novel systems biology approaches, understanding of variability both experimental and interindividual (pharmacogenomic), and case studies that provide real life examples of applying these principles. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics especially suitable for the novice, in some cases step-by-step, readily reproducible protocols, and insights to help with troubleshooting and avoiding known pitfalls with extensive cross referencing to assist in learning. Authoritative and easily accessible, Enzyme Kinetics in Drug Metabolism: Fundamentals and Applications serves as a very practical teaching tool for novice, non-mathematically trained scientists interested in these fundamental concepts and as an aid for their supervisors in teaching these principles.
Methods To Determine Enzymatic Activity
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Author : Alane Beatriz Vermelho
language : en
Publisher: Bentham Science Publishers
Release Date : 2013-11-22
Methods To Determine Enzymatic Activity written by Alane Beatriz Vermelho and has been published by Bentham Science Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-22 with Science categories.
Methods to Determine Enzymatic Activity is a textbook about industrial enzymes. The book features definitions, classifications and applications of selected enzymes important in industry and in biotechnological processes. Analytical methods for these enzymes are also included in the text. The main objective of this textbook is to provide readers information focused on the current analysis methods of enzymatic activity at qualitative and quantitative levels. Each chapter is about one specific enzyme and contains information about its substrate and some biochemical properties. The methodologies are presented as an experimental protocol allowing interested readers to reproduce the experimental methods detailed within the textbook. These protocols contain the principle of the technique, materials, methods, and all steps necessary for the determination of enzyme activity and interpretation of results. Each methodology is illustrated with photos and schemes for a better and clear understanding. This book, therefore, uniquely brings modern analysis techniques of industrial enzymes in a single easy to understand volume. This textbook is suitable for undergraduate enzymology courses and advanced industrial biotechnology and microbiology courses.