Pyrosequencing Protocols
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Pyrosequencing Protocols
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Author : Sharon Marsh
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Pyrosequencing Protocols written by Sharon Marsh and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing®.
Pharmacogenomics
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Author : Federico Innocenti
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Pharmacogenomics written by Federico Innocenti and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Medical categories.
Expert researchers and physician/clinicians describe in detail the newest and most commonly used technologies today in this rapidly advancing field. The authors provide readily reproducible methods for assessing the functional consequences of a certain polymorphism, evaluate the variety of genotyping platforms currently available, and discuss the management of pharmacogenomic information. Highlights include techniques for making a snapshot of the allele-specific variation in human gene expression, genome wide analysis of allele-specific expression using oligo microarrays, in vivo assays with HaploChIP, SNP genotyping in DNA pools, and PharmGKB, the pharmacogenetics and pharmacogenomics knowledge base. The methodologies for genotyping include denaturing high-performance liquid chromarography, pyrosequencing, kinetic-fluorescence detection, mass spectrometry, and TaqMan assay for insertion/deletions.
Basic Methods And Protocols On Sourdough
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Author : Marco Gobbetti
language : en
Publisher: Springer Nature
Release Date : 2024-02-19
Basic Methods And Protocols On Sourdough written by Marco Gobbetti and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-02-19 with Technology & Engineering categories.
This volume details traditional and modern approaches to characterize sourdough and to monitor its features during production, propagation and use, through state-of- the art analytical tools. Chapters guide reader through procedures to prepare and propagate the mature sourdough, to analyse and monitor microbiological, biochemical and rheology features of sourdough, sensory, and nutritional attributes of baked goods. Written in the format of the Methods and Protocols in Food Science series, chapters list necessary materials and methods for readily reproducible protocols. Authoritative and cutting-edge, Basic Methods and Protocols on Sourdough aims to ensure successful results in the further study of this vital field.
Epigenomics From Chromatin Biology To Therapeutics
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Author : Krishnarao Appasani
language : en
Publisher: Cambridge University Press
Release Date : 2012
Epigenomics From Chromatin Biology To Therapeutics written by Krishnarao Appasani and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Epigenetics categories.
Experts from academia, the biotechnology and pharmaceutical industries introduce biological, medical and methodological aspects of the emerging field of epigenomics.
Epigenomics
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Author : Izuho Hatada
language : en
Publisher: Springer Nature
Release Date : 2022-09-29
Epigenomics written by Izuho Hatada and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-09-29 with Science categories.
This detailed volume provides a comprehensive collection of protocols for epigenomic research, powering our ability to analyze epigenetic modifications across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for epigenome editing, and epigenetically modified animals. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenomics: Methods and Protocols serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research.
Epigenetics Methods
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Author : Trygve O. Tollefsbol
language : en
Publisher: Academic Press
Release Date : 2020-07-08
Epigenetics Methods written by Trygve O. Tollefsbol and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-08 with Medical categories.
In recent years, the field of epigenetics has grown significantly, driving new understanding of human developmental processes and disease expression, as well as advances in diagnostics and therapeutics. As the field of epigenetics continues to grow, methods and technologies have multiplied, resulting in a wide range of approaches and tools researchers might employ. Epigenetics Methods offers comprehensive instruction in methods, protocols, and experimental approaches applied in field of epigenetics. Here, across thirty-five chapters, specialists offer step-by-step overviews of methods used to study various epigenetic mechanisms, as employed in basic and translational research. Leading the reader from fundamental to more advanced methods, the book begins with thorough instruction in DNA methylation techniques and gene or locus-specific methylation analyses, followed by histone modification methods, chromatin evaluation, enzyme analyses of histone methylation, and studies of non-coding RNAs as epigenetic modulators. Recently developed techniques and technologies discussed include single-cell epigenomics, epigenetic editing, computational epigenetics, systems biology epigenetic methods, and forensic epigenetic approaches. Epigenetics methods currently in-development, and their implication for future research, are also considered in-depth. In addition, as with the wider life sciences, reproducibility across experiments, labs, and subdisciplines is a growing issue for epigenetics researchers. This volume provides consensus-driven methods instruction and overviews. Tollefsbol and contributing authors survey the range of existing methods; identify best practices, common themes, and challenges; and bring unity of approach to a diverse and ever-evolving field. - Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application - Integrates technology and translation with fundamental chapters on epigenetics methods, as well as chapters on more novel and advanced epigenetics methods - Written at verbal and technical levels that can be understood by scientists and students alike - Includes chapters on state-of-the-art techniques such as single-cell epigenomics, use of CRISPR/Cas9 for epigenetic editing, and epigenetics methods applied to forensics
Genetics Genomics And Breeding Of Tomato
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Author : Barbara E. Liedl
language : en
Publisher: CRC Press
Release Date : 2013-01-17
Genetics Genomics And Breeding Of Tomato written by Barbara E. Liedl and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-01-17 with Science categories.
This volume covers the advances in the study of tomato diversity and taxonomy. It examines the mapping of simple and complex traits, classical genetics and breeding, association studies, molecular breeding, positional cloning, and structural and comparative genomics. The contributors also discuss transcriptomics, proteomics, metabolomics, and bioin
Genomics
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Author : Supratim Choudhuri
language : en
Publisher: CRC Press
Release Date : 2008-10-09
Genomics written by Supratim Choudhuri and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-10-09 with Medical categories.
This unique new text delivers a solid foundation for understanding the role of genomics in human health and in advances that promise to help improve the quality of human life. Unlike other works that focus mainly on toxicogenomic techniques, Genomics presents a thorough overview of the field in four major sections: 1) fundamentals of genes and geno
Microarrays
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Author : Jang B. Rampal
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Microarrays written by Jang B. Rampal and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
This second part in the two-volume work Microarrays details applications and data analysis. It includes insight into non-mammalian vertebrate systems, processes and protocols for high quality glass-based microarrays. Coverage includes applications in DNA, peptide, antibody and carbohydrate microarraying, oligonucleotide microarrays generated from hydrolysis PCR probe sequences, microarray platforms in clinical practice, and screening of cDNA libraries on glass slide microarrays. Authors in this volume also discuss protocols for predicting DNA duplex stability on oligonucleotide arrays and integrated analysis of microarray results.
Emerging Infectious Diseases
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Author :
language : en
Publisher:
Release Date : 2012
Emerging Infectious Diseases written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Communicable diseases categories.