Quantitative Imaging In Cell Biology
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Quantitative Imaging In Cell Biology
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Author :
language : en
Publisher: Academic Press
Release Date : 2014-06-25
Quantitative Imaging In Cell Biology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-25 with Science categories.
This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Fluorescence Microscopy Of Living Cells In Culture Part B
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Author :
language : en
Publisher: Academic Press
Release Date : 1989-04-01
Fluorescence Microscopy Of Living Cells In Culture Part B written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-04-01 with Science categories.
Fluorescence Microscopy of Living Cells in Culture, Part B
Multi Parametric Live Cell Microscopy Of 3d Tissue Models
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Author : Ruslan I. Dmitriev
language : en
Publisher: Springer
Release Date : 2017-10-26
Multi Parametric Live Cell Microscopy Of 3d Tissue Models written by Ruslan I. Dmitriev and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-26 with Science categories.
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
Cell Imaging Techniques
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Author : Douglas J. Taatjes
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Cell Imaging Techniques written by Douglas J. Taatjes and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Medical categories.
A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Quantitative Imaging
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Author : JENS. RITTSCHER
language : en
Publisher:
Release Date : 2019
Quantitative Imaging written by JENS. RITTSCHER and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with categories.
Quantitative Bioimaging
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Author : Raimund J. Ober
language : en
Publisher: CRC Press
Release Date : 2020-12-15
Quantitative Bioimaging written by Raimund J. Ober and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-12-15 with Medical categories.
Quantitative bioimaging is a broad interdisciplinary field that exploits tools from biology, chemistry, optics, and statistical data analysis for the design and implementation of investigations of biological processes. Instead of adopting the traditional approach of focusing on just one of the component disciplines, this textbook provides a unique introduction to quantitative bioimaging that presents all of the disciplines in an integrated manner. The wide range of topics covered include basic concepts in molecular and cellular biology, relevant aspects of antibody technology, instrumentation and experimental design in fluorescence microscopy, introductory geometrical optics and diffraction theory, and parameter estimation and information theory for the analysis of stochastic data. Key Features: Comprises four parts, the first of which provides an overview of the topics that are developed from fundamental principles to more advanced levels in the other parts. Presents in the second part an in-depth introduction to the relevant background in molecular and cellular biology and in physical chemistry, which should be particularly useful for students without a formal background in these subjects. Provides in the third part a detailed treatment of microscopy techniques and optics, again starting from basic principles. Introduces in the fourth part modern statistical approaches to the determination of parameters of interest from microscopy data, in particular data generated by single molecule microscopy experiments. Uses two topics related to protein trafficking (transferrin trafficking and FcRn-mediated antibody trafficking) throughout the text to motivate and illustrate microscopy techniques. An online appendix providing the background and derivations for various mathematical results presented or used in the text is available at http://www.routledge.com/9781138598980.
Imaging Flow Cytometry
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Author : Natasha S. Barteneva
language : en
Publisher: Humana Press
Release Date : 2016-08-23
Imaging Flow Cytometry written by Natasha S. Barteneva and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Medical categories.
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
Artificial Intelligence In Label Free Microscopy
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Author : Ata Mahjoubfar
language : en
Publisher: Springer
Release Date : 2017-04-19
Artificial Intelligence In Label Free Microscopy written by Ata Mahjoubfar and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-04-19 with Technology & Engineering categories.
This book introduces time-stretch quantitative phase imaging (TS-QPI), a high-throughput label-free imaging flow cytometer developed for big data acquisition and analysis in phenotypic screening. TS-QPI is able to capture quantitative optical phase and intensity images simultaneously, enabling high-content cell analysis, cancer diagnostics, personalized genomics, and drug development. The authors also demonstrate a complete machine learning pipeline that performs optical phase measurement, image processing, feature extraction, and classification, enabling high-throughput quantitative imaging that achieves record high accuracy in label -free cellular phenotypic screening and opens up a new path to data-driven diagnosis.
Quantitative Fluorescence Microscopy
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Author : F. W. D. Rost
language : en
Publisher: Cambridge University Press
Release Date : 1991-07-26
Quantitative Fluorescence Microscopy written by F. W. D. Rost and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-07-26 with Science categories.
Quantitative fluorescence microscopy is concerned with making measurements from fluorescent specimens in a fluorescence microscope, by measuring fluorescence emission from a defined area or areas of a specimen. This technique is most commonly used to determine the amount of some specific substance, such as DNA, in some particular area of a cell. But it has many other uses; for example, it can be used to identify certain substances in the cell by examining their fluorescence characteristics. This book is a complete guide to this technique for all biologists. It describes the principles and applications of quantitative fluorescence microscopy and also gives much practical information about the instrumentation required. There is also a discussion of the exciting developments in confocal fluorescence microscopy which allows the three dimensional distribution of particular substances to be determined. Everyone presently using this technique, or wishing to start using it will need to read this book.
Optical Imaging Techniques In Cell Biology Second Edition
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Author : Guy Cox
language : en
Publisher: CRC Press
Release Date : 2012-06-04
Optical Imaging Techniques In Cell Biology Second Edition written by Guy Cox and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-04 with Medical categories.
Optical Imaging Techniques in Cell Biology, Second Edition covers the field of biological microscopy, from the optics of the microscope to the latest advances in imaging below the traditional resolution limit. It includes the techniques—such as labeling by immunofluorescence and fluorescent proteins—which have revolutionized cell biology. Quantitative techniques such as lifetime imaging, ratiometric measurement, and photoconversion are all covered in detail. Expanded with a new chapter and 40 new figures, the second edition has been updated to cover the latest developments in optical imaging techniques. Explanations throughout are accurate, detailed, but as far as possible non-mathematical. This edition includes appendices with useful practical protocols, references, and suggestions for further reading. Color figures are integrated throughout.